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The map label for this gene is paaJ [H]

Identifier: 154244866

GI number: 154244866

Start: 1029782

End: 1030984

Strand: Direct

Name: paaJ [H]

Synonym: Xaut_0916

Alternate gene names: 154244866

Gene position: 1029782-1030984 (Clockwise)

Preceding gene: 154244865

Following gene: 154244867

Centisome position: 19.4

GC content: 71.9

Gene sequence:

>1203_bases
ATGACCGACGCCTTCGTCTGCGATTTCATCCGCACCCCCATTGGCCGCTATGGCGGCGCGCTCGCGCCGGTGCGCGCCGA
CGACCTCGCCGCGCTGCCACTCGGCGCCCTCCTTGCCCGCAATCCCGGCCTCGATCCCGCCGCCGTGGAGGAGGTGTGGA
TGGGCTGCGCCAACCAGGCGGGCGAGGACAACCGCAACGTGGCGCGCATGGCGCTGCTGCTCGCCGGCCTGCCGCAGAGC
GTGCCCGGCGTCACGGTCAACCGCTTGTGCGGCTCCGGGCTGGAAGCAGTGGCGGCGGCGGCCCGCGCCATAAGGTCCGG
CGACATGGAGCTCGCCATCGCCGGTGGGGTTGAAAGCATGACCCGTGCGCCCTTCGTGATGCCCAAGGGATCGTCGGCGT
GGTCTCGCGCGACGGAGATCCACGACACCACCATCGGCTGGCGCTTCGTCAATCCCCGCATGGCGCAGGACTACGGCACT
GATTCCATGCCCGAGACGGCGCAGAATCTGGCCGACGAACACGCCATCAGCCGCGCCGACCAGGACGCCTTCGCCCTCAG
GTCCCAGGAGAAGGCGGCGGCGGCCCAGGCGAGCGGCCGGCTTGCCGCCGAGATCCTGCCCGTCAGCGTGCCGCTCGGCC
GTGGCAAGACGGAAGAGGTGGCGAAAGACGAGCACCCGCGCGCCACGACCCTCGCCGATCTCGCTAAGCTCAAGCCCATC
ACCCGGCCCGATGGCTCGGTGACCGCCGGCAATGCCAGCGGCGTCAATGACGGGGCGGCGGCGCTCATCGTGGCTTCGCA
GGCGGCGGCGAAGCGCTTCGGGCTCACCCCCCTTGCCCGTGTGGTGGCCGGGGCGTCGGCGGGTGTCGCGCCGCGCATCA
TGGGCATTGGTCCCGTGCCGGCGGTGGGGAAGCTCTGCGCAAAGACCGGGGTCGGGCTGGAGGCCATCGACATCATCGAG
CTGAACGAGGCCTTCGCCGCCCAAGCCCTCGCGGTGCTGCGCGCGCTTCGCCTCAGCGACGACGATCCGCGGGTGAACCC
AAATGGCGGCGCCATCGCGCTCGGCCATCCCCTCGGCATGAGCGGGGCGCGCATCGCCGGATCGGCGGCGCTGGAGCTGG
CGCGTGGCGCGGGCCGCCATGCGCTGGCGACCATGTGCGTGGGCGTGGGCCAGGGCGCCGCGCTGTTGCTGGAGCGGATT
TGA

Upstream 100 bases:

>100_bases
AGGCCTTCGCCGTCGAGGATCCGCTGTCCTGGGGCGTTCCCGAGCTCCTGCGCCGTGCGGCGCAGGAGGGCCTGAGCCCC
GACGACCTGAAAGCTTCCCA

Downstream 100 bases:

>100_bases
CGACGTGGGACGCAGGCCGACGCGACTTGGGACGTAAAGCTCAAGCAGAAGGATGCCCCCGACCTGCCGGCTGTCCAGCG
GGATGAGGTGGTCCCGCCCT

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTDAFVCDFIRTPIGRYGGALAPVRADDLAALPLGALLARNPGLDPAAVEEVWMGCANQAGEDNRNVARMALLLAGLPQS
VPGVTVNRLCGSGLEAVAAAARAIRSGDMELAIAGGVESMTRAPFVMPKGSSAWSRATEIHDTTIGWRFVNPRMAQDYGT
DSMPETAQNLADEHAISRADQDAFALRSQEKAAAAQASGRLAAEILPVSVPLGRGKTEEVAKDEHPRATTLADLAKLKPI
TRPDGSVTAGNASGVNDGAAALIVASQAAAKRFGLTPLARVVAGASAGVAPRIMGIGPVPAVGKLCAKTGVGLEAIDIIE
LNEAFAAQALAVLRALRLSDDDPRVNPNGGAIALGHPLGMSGARIAGSAALELARGAGRHALATMCVGVGQGAALLLERI

Sequences:

>Translated_400_residues
MTDAFVCDFIRTPIGRYGGALAPVRADDLAALPLGALLARNPGLDPAAVEEVWMGCANQAGEDNRNVARMALLLAGLPQS
VPGVTVNRLCGSGLEAVAAAARAIRSGDMELAIAGGVESMTRAPFVMPKGSSAWSRATEIHDTTIGWRFVNPRMAQDYGT
DSMPETAQNLADEHAISRADQDAFALRSQEKAAAAQASGRLAAEILPVSVPLGRGKTEEVAKDEHPRATTLADLAKLKPI
TRPDGSVTAGNASGVNDGAAALIVASQAAAKRFGLTPLARVVAGASAGVAPRIMGIGPVPAVGKLCAKTGVGLEAIDIIE
LNEAFAAQALAVLRALRLSDDDPRVNPNGGAIALGHPLGMSGARIAGSAALELARGAGRHALATMCVGVGQGAALLLERI
>Mature_399_residues
TDAFVCDFIRTPIGRYGGALAPVRADDLAALPLGALLARNPGLDPAAVEEVWMGCANQAGEDNRNVARMALLLAGLPQSV
PGVTVNRLCGSGLEAVAAAARAIRSGDMELAIAGGVESMTRAPFVMPKGSSAWSRATEIHDTTIGWRFVNPRMAQDYGTD
SMPETAQNLADEHAISRADQDAFALRSQEKAAAAQASGRLAAEILPVSVPLGRGKTEEVAKDEHPRATTLADLAKLKPIT
RPDGSVTAGNASGVNDGAAALIVASQAAAKRFGLTPLARVVAGASAGVAPRIMGIGPVPAVGKLCAKTGVGLEAIDIIEL
NEAFAAQALAVLRALRLSDDDPRVNPNGGAIALGHPLGMSGARIAGSAALELARGAGRHALATMCVGVGQGAALLLERI

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=41.7721518987342, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=40.2035623409669, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=36.5671641791045, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=40.1985111662531, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI4504327, Length=430, Percent_Identity=31.3953488372093, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI194353979, Length=398, Percent_Identity=29.3969849246231, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=66.0847880299252, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=43.5960591133005, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=44.5, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.1612903225806, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=31.6037735849057, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=42.8927680798005, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI25147385, Length=399, Percent_Identity=35.3383458646617, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=410, Percent_Identity=35.609756097561, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=28.8372093023256, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=29.4554455445545, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=37.3096446700508, Blast_Score=205, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=34.2431761786601, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24655093, Length=406, Percent_Identity=44.0886699507389, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI17648125, Length=397, Percent_Identity=41.0579345088161, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=35.7142857142857, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=31.6901408450704, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 40796; Mature: 40665

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAFVCDFIRTPIGRYGGALAPVRADDLAALPLGALLARNPGLDPAAVEEVWMGCANQA
CCCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMALLLAGLPQSVPGVTVNRLCGSGLEAVAAAARAIRSGDMELAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCHHHH
TRAPFVMPKGSSAWSRATEIHDTTIGWRFVNPRMAQDYGTDSMPETAQNLADEHAISRAD
HCCCEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHCC
QDAFALRSQEKAAAAQASGRLAAEILPVSVPLGRGKTEEVAKDEHPRATTLADLAKLKPI
CHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHCCC
TRPDGSVTAGNASGVNDGAAALIVASQAAAKRFGLTPLARVVAGASAGVAPRIMGIGPVP
CCCCCCEECCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCCCH
AVGKLCAKTGVGLEAIDIIELNEAFAAQALAVLRALRLSDDDPRVNPNGGAIALGHPLGM
HHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCC
SGARIAGSAALELARGAGRHALATMCVGVGQGAALLLERI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TDAFVCDFIRTPIGRYGGALAPVRADDLAALPLGALLARNPGLDPAAVEEVWMGCANQA
CCHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMALLLAGLPQSVPGVTVNRLCGSGLEAVAAAARAIRSGDMELAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCHHHH
TRAPFVMPKGSSAWSRATEIHDTTIGWRFVNPRMAQDYGTDSMPETAQNLADEHAISRAD
HCCCEEECCCCCHHHHHHHHCCCCCEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHCC
QDAFALRSQEKAAAAQASGRLAAEILPVSVPLGRGKTEEVAKDEHPRATTLADLAKLKPI
CHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHCCC
TRPDGSVTAGNASGVNDGAAALIVASQAAAKRFGLTPLARVVAGASAGVAPRIMGIGPVP
CCCCCCEECCCCCCCCCCCEEEEEEHHHHHHHHCCCHHHHHHHCCCCCCCCEEECCCCCH
AVGKLCAKTGVGLEAIDIIELNEAFAAQALAVLRALRLSDDDPRVNPNGGAIALGHPLGM
HHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCC
SGARIAGSAALELARGAGRHALATMCVGVGQGAALLLERI
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]