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The map label for this gene is vanX [H]

Identifier: 154244605

GI number: 154244605

Start: 734510

End: 735307

Strand: Direct

Name: vanX [H]

Synonym: Xaut_0653

Alternate gene names: 154244605

Gene position: 734510-735307 (Clockwise)

Preceding gene: 154244603

Following gene: 154244615

Centisome position: 13.84

GC content: 68.92

Gene sequence:

>798_bases
GTGCTGTGCACAAGCAGGGACACACCCGCCCTCCCGCCGGTTTTCTTCGGCGCGGGACGCTCATATGATTGCGCACCATC
TCCCGCGATCCGCGCCATGCCCTCTTTCCTGATGGACCGACTGGTGAAGCCCGCTGCCCTTGCCTTGAGCATCGCCGGCC
TGCCGCTGGCGGCCGATGCCCGCACCGACCGTCCGGCCCTGTTCGTGGACGTGGCCACGCTGGTGCCGGACGCCGTGTTC
GACGTGCGTTATTTCAGCTCGGACAATTTCGTCGGCACGCGGGTGGACGGCTACGGCGCCGCGCGCTGCTTCCTCACCCG
CCCGGCCGCCGAAGCGCTGGCCGCCGTGGCGGCGGAGGCGAAGGCCGCCGGGCTTGGCCTGCGCCTGTTCGATTGCTACC
GCCCCGCCCGCGCGGTGGCGCACTTTGCCCGCTGGGCCAGCGATCTCGCCGACACCCGCACCAAGGCCGCCTTCTATCCC
GACGTGGACAAGAAGGACCTGTTCGCCGAAGGCTATATCGCCGCCCGCTCCGGCCACTCGCGCGGCTCGACCCTGGACCT
GACCCTGTTCGATCGCGCCACCGGGGCCGATCTCGACATGGGCACCCCCTTCGACCTGTTCAGCCCGCGCTCGTGGCCGG
GCTCGGATGCGGTGACGAGGGCGCAGAAGGCCAATCGCGACCGGCTCGCCGGCTTCATGGCGCGGCACGGCTTCCAGCCG
TTCGACAAGGAATGGTGGCACTTCACCCTGAAGGCCGAACCCTTCCCCGACACCTATTTCGATTTCCCCATCGAATAG

Upstream 100 bases:

>100_bases
CTGCTTCGACCCGGCACGGTGGGACCCTCCTCCCGCCCGAAGCCAGATGTTTCCGGCTTTGGTCCTTGGGAACTGAACTT
GCGCGCCGGGGGGCGGCCTG

Downstream 100 bases:

>100_bases
GCGGGCGGCGACGCCAGAGTTCAGTTCGGGGAGATTTCCTCCACCTTCAGCCGGCGGCCTTGGGCGTCGAAGAAATCGCC
CTGGTTGATCACCAGCCGGT

Product: peptidase M15D vanX D-ala-D-ala dipeptidase

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLCTSRDTPALPPVFFGAGRSYDCAPSPAIRAMPSFLMDRLVKPAALALSIAGLPLAADARTDRPALFVDVATLVPDAVF
DVRYFSSDNFVGTRVDGYGAARCFLTRPAAEALAAVAAEAKAAGLGLRLFDCYRPARAVAHFARWASDLADTRTKAAFYP
DVDKKDLFAEGYIAARSGHSRGSTLDLTLFDRATGADLDMGTPFDLFSPRSWPGSDAVTRAQKANRDRLAGFMARHGFQP
FDKEWWHFTLKAEPFPDTYFDFPIE

Sequences:

>Translated_265_residues
MLCTSRDTPALPPVFFGAGRSYDCAPSPAIRAMPSFLMDRLVKPAALALSIAGLPLAADARTDRPALFVDVATLVPDAVF
DVRYFSSDNFVGTRVDGYGAARCFLTRPAAEALAAVAAEAKAAGLGLRLFDCYRPARAVAHFARWASDLADTRTKAAFYP
DVDKKDLFAEGYIAARSGHSRGSTLDLTLFDRATGADLDMGTPFDLFSPRSWPGSDAVTRAQKANRDRLAGFMARHGFQP
FDKEWWHFTLKAEPFPDTYFDFPIE
>Mature_265_residues
MLCTSRDTPALPPVFFGAGRSYDCAPSPAIRAMPSFLMDRLVKPAALALSIAGLPLAADARTDRPALFVDVATLVPDAVF
DVRYFSSDNFVGTRVDGYGAARCFLTRPAAEALAAVAAEAKAAGLGLRLFDCYRPARAVAHFARWASDLADTRTKAAFYP
DVDKKDLFAEGYIAARSGHSRGSTLDLTLFDRATGADLDMGTPFDLFSPRSWPGSDAVTRAQKANRDRLAGFMARHGFQP
FDKEWWHFTLKAEPFPDTYFDFPIE

Specific function: Hydrolyzes the cellular pool of D-Ala-D-Ala dipeptides. May play a role in immunity or defense against glycopeptide antibiotics (perhaps at a moderate level) in the soil environment. Might confer vancomycin resistance to S.coelicolor [H]

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=190, Percent_Identity=34.7368421052632, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 28947; Mature: 28947

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCTSRDTPALPPVFFGAGRSYDCAPSPAIRAMPSFLMDRLVKPAALALSIAGLPLAADA
CCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCC
RTDRPALFVDVATLVPDAVFDVRYFSSDNFVGTRVDGYGAARCFLTRPAAEALAAVAAEA
CCCCCEEEEEHHHHCCHHHHEEEEECCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHH
KAAGLGLRLFDCYRPARAVAHFARWASDLADTRTKAAFYPDVDKKDLFAEGYIAARSGHS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHCCEEEECCCCC
RGSTLDLTLFDRATGADLDMGTPFDLFSPRSWPGSDAVTRAQKANRDRLAGFMARHGFQP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCC
FDKEWWHFTLKAEPFPDTYFDFPIE
CCCCCEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLCTSRDTPALPPVFFGAGRSYDCAPSPAIRAMPSFLMDRLVKPAALALSIAGLPLAADA
CCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCEECCC
RTDRPALFVDVATLVPDAVFDVRYFSSDNFVGTRVDGYGAARCFLTRPAAEALAAVAAEA
CCCCCEEEEEHHHHCCHHHHEEEEECCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHH
KAAGLGLRLFDCYRPARAVAHFARWASDLADTRTKAAFYPDVDKKDLFAEGYIAARSGHS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHCCEEEECCCCC
RGSTLDLTLFDRATGADLDMGTPFDLFSPRSWPGSDAVTRAQKANRDRLAGFMARHGFQP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCC
FDKEWWHFTLKAEPFPDTYFDFPIE
CCCCCEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11881895; 12000953 [H]