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The map label for this gene is 154244261

Identifier: 154244261

GI number: 154244261

Start: 311373

End: 312794

Strand: Reverse

Name: 154244261

Synonym: Xaut_0304

Alternate gene names: NA

Gene position: 312794-311373 (Counterclockwise)

Preceding gene: 154244264

Following gene: 154244260

Centisome position: 5.89

GC content: 65.33

Gene sequence:

>1422_bases
ATGGCAATCGGTGAGTTCGCGAGCGCGCTGCGTTTACCCCGCCAGGGTGACGTCGGCCTGTCGACCCCGCTCTACTGGAT
GTATGAGATGGGTTACGCTGCGCTCAACCCGTCGCGGGCGGTGGCGGATGCCAGCCGGGCGGTCCTCAACAACCCCATCA
ATCCGGTCTCCCACACCACCCTCGGCAAGTCCATGGCTGCCGCCTGCGAGCTGTTCGAGCGCACCACGCGGCGCTATGGC
AAGCCGGACTGGGGCATCACCCACACCACCGTGGGCGGGCAGAAGGTGCCGGTGCATATCAACACAGTGTGGCAGCGTCC
GTTCTGCAACCTGCTGCATTTCGAGCGCGCGTTCGAATATCCGCCGCGCGGGCCGCAGCCGCGCCTGCTCATCGTGGCCC
CGGTGTCCGGCCACCACGCCACCCTGCTGCGCGGCACGGTGGAAGCCATGCTGCCCACCCACGATGTCTATGTCACAGAC
TGGGTCGATGCCAAGCTCGTGCCCCTCACCGATGGCCGCTTCAGCCTGTCGGATTATGTCGACTACCTCATCTCCATCTT
CCATCGCATGGGCGGCGACTGCCATGTGATGGCGGTGTGCCAGCCGGCGGTGCCGGTGATGATGGCGGTGGCGCGCATGG
AGGCGGAGGAAGACCCCTTCGTGCCCAAGTCCATGATTCTCATGGGCGGCCCCATCGACACCCGCGAGGCGCCGACGGCG
GTGAACGCCCTGGCCAAGGATCGCGGCATCGACTGGTTCAAGCGCCACGTGCTCACCACCGTGCCGTGGCCCCATGCCGG
TGCCATGCGGGAGGTCTATCCCGGCTTCCTGCAGCTCAACGGCTTCATGTCCATGAATCTGGACCGCCACCTGCAGGCTC
ATTGGGACCTGTTCCACCATCTGGTGCAGGGCGACGGCGATTCGGCCGAGAAGCACCGTGACTTCTACGACGAATACCTG
GCGGTGATGGACCTGACGGCCGAGTTCTATATTGAAACGGTTGAGAACGTGTTCATCACCCACGCCCTTCCCAAGGGTGA
GATCCTGCACCATTCCCAGCCGGTGAAGCCCGACGCCATCCACCGCGTCGCGCTGATGACGGTGGAGGGCGAGAAGGACG
ACATTTCGGGCATCGGCCAGACCAAGGCGGCGCACAAGATCTGCCCGCGCATTCCCGAGGACAAGCGCGCCCACTACGAG
CAGCCGGGCGTCGGCCATTACGGCGTGTTCAACGGCTCGCGCTTCCGCTCGGAGATCGCCCCGCGCATCGCCGACTTCAT
CCTGTCCCAGGAACTGCCCCGCGGCGCCACCGACCATGTGACCAGCGCACGGCCGGCGCCCGAGGCGGTTGAGGACGGCC
CGGACATGTTTGCCCTCAACAAGAATGTGACCGCCTTTTATCCCCGGCTTGCTCCGACTTAG

Upstream 100 bases:

>100_bases
ACTCTATGTCTTTTGTCGGCATTGACCAAACCTGCAAGTGCGCTCACGTTTTGGGGAAGGCGCCACCCGTAACGGAGCGC
ACTAATCGTGAGGAGCGCAG

Downstream 100 bases:

>100_bases
CACTGGCAGGGAGAGCCATTTGAGGGGAAGATGCGGTGGTAACGTCGCCCCGGTTGCCGCCATGCTGTTTCGCCGTCAGG
ACCCAAAGCCGCCCGCGCAG

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; Poly(3-Hydroxybutyrate) Depolymerase; Alpha/Beta Hydrolase Fold; Alpha/Beta Hydrolase Fold Protein; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; PHB De-Polymerase Domain Protein; PHB De-Polymerase; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAIGEFASALRLPRQGDVGLSTPLYWMYEMGYAALNPSRAVADASRAVLNNPINPVSHTTLGKSMAAACELFERTTRRYG
KPDWGITHTTVGGQKVPVHINTVWQRPFCNLLHFERAFEYPPRGPQPRLLIVAPVSGHHATLLRGTVEAMLPTHDVYVTD
WVDAKLVPLTDGRFSLSDYVDYLISIFHRMGGDCHVMAVCQPAVPVMMAVARMEAEEDPFVPKSMILMGGPIDTREAPTA
VNALAKDRGIDWFKRHVLTTVPWPHAGAMREVYPGFLQLNGFMSMNLDRHLQAHWDLFHHLVQGDGDSAEKHRDFYDEYL
AVMDLTAEFYIETVENVFITHALPKGEILHHSQPVKPDAIHRVALMTVEGEKDDISGIGQTKAAHKICPRIPEDKRAHYE
QPGVGHYGVFNGSRFRSEIAPRIADFILSQELPRGATDHVTSARPAPEAVEDGPDMFALNKNVTAFYPRLAPT

Sequences:

>Translated_473_residues
MAIGEFASALRLPRQGDVGLSTPLYWMYEMGYAALNPSRAVADASRAVLNNPINPVSHTTLGKSMAAACELFERTTRRYG
KPDWGITHTTVGGQKVPVHINTVWQRPFCNLLHFERAFEYPPRGPQPRLLIVAPVSGHHATLLRGTVEAMLPTHDVYVTD
WVDAKLVPLTDGRFSLSDYVDYLISIFHRMGGDCHVMAVCQPAVPVMMAVARMEAEEDPFVPKSMILMGGPIDTREAPTA
VNALAKDRGIDWFKRHVLTTVPWPHAGAMREVYPGFLQLNGFMSMNLDRHLQAHWDLFHHLVQGDGDSAEKHRDFYDEYL
AVMDLTAEFYIETVENVFITHALPKGEILHHSQPVKPDAIHRVALMTVEGEKDDISGIGQTKAAHKICPRIPEDKRAHYE
QPGVGHYGVFNGSRFRSEIAPRIADFILSQELPRGATDHVTSARPAPEAVEDGPDMFALNKNVTAFYPRLAPT
>Mature_472_residues
AIGEFASALRLPRQGDVGLSTPLYWMYEMGYAALNPSRAVADASRAVLNNPINPVSHTTLGKSMAAACELFERTTRRYGK
PDWGITHTTVGGQKVPVHINTVWQRPFCNLLHFERAFEYPPRGPQPRLLIVAPVSGHHATLLRGTVEAMLPTHDVYVTDW
VDAKLVPLTDGRFSLSDYVDYLISIFHRMGGDCHVMAVCQPAVPVMMAVARMEAEEDPFVPKSMILMGGPIDTREAPTAV
NALAKDRGIDWFKRHVLTTVPWPHAGAMREVYPGFLQLNGFMSMNLDRHLQAHWDLFHHLVQGDGDSAEKHRDFYDEYLA
VMDLTAEFYIETVENVFITHALPKGEILHHSQPVKPDAIHRVALMTVEGEKDDISGIGQTKAAHKICPRIPEDKRAHYEQ
PGVGHYGVFNGSRFRSEIAPRIADFILSQELPRGATDHVTSARPAPEAVEDGPDMFALNKNVTAFYPRLAPT

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52737; Mature: 52606

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGEFASALRLPRQGDVGLSTPLYWMYEMGYAALNPSRAVADASRAVLNNPINPVSHTT
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHHCCCCCCCHHHH
LGKSMAAACELFERTTRRYGKPDWGITHTTVGGQKVPVHINTVWQRPFCNLLHFERAFEY
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHCC
PPRGPQPRLLIVAPVSGHHATLLRGTVEAMLPTHDVYVTDWVDAKLVPLTDGRFSLSDYV
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHH
DYLISIFHRMGGDCHVMAVCQPAVPVMMAVARMEAEEDPFVPKSMILMGGPIDTREAPTA
HHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCCCCCHH
VNALAKDRGIDWFKRHVLTTVPWPHAGAMREVYPGFLQLNGFMSMNLDRHLQAHWDLFHH
HHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCEEECCHHHHHHHHHHHHHH
LVQGDGDSAEKHRDFYDEYLAVMDLTAEFYIETVENVFITHALPKGEILHHSQPVKPDAI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHH
HRVALMTVEGEKDDISGIGQTKAAHKICPRIPEDKRAHYEQPGVGHYGVFNGSRFRSEIA
EEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHCCCCCCCCCEECCCCHHHHHHHH
PRIADFILSQELPRGATDHVTSARPAPEAVEDGPDMFALNKNVTAFYPRLAPT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCEEECCCCCCC
>Mature Secondary Structure 
AIGEFASALRLPRQGDVGLSTPLYWMYEMGYAALNPSRAVADASRAVLNNPINPVSHTT
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHCCCHHHHHHHHHHHHCCCCCCCHHHH
LGKSMAAACELFERTTRRYGKPDWGITHTTVGGQKVPVHINTVWQRPFCNLLHFERAFEY
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEEEECCCHHHHHHHHHHHHCC
PPRGPQPRLLIVAPVSGHHATLLRGTVEAMLPTHDVYVTDWVDAKLVPLTDGRFSLSDYV
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCCHHHHH
DYLISIFHRMGGDCHVMAVCQPAVPVMMAVARMEAEEDPFVPKSMILMGGPIDTREAPTA
HHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCCCCCHH
VNALAKDRGIDWFKRHVLTTVPWPHAGAMREVYPGFLQLNGFMSMNLDRHLQAHWDLFHH
HHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCCEEECCEEECCHHHHHHHHHHHHHH
LVQGDGDSAEKHRDFYDEYLAVMDLTAEFYIETVENVFITHALPKGEILHHSQPVKPDAI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHH
HRVALMTVEGEKDDISGIGQTKAAHKICPRIPEDKRAHYEQPGVGHYGVFNGSRFRSEIA
EEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCHHHHCCCCCCCCCEECCCCHHHHHHHH
PRIADFILSQELPRGATDHVTSARPAPEAVEDGPDMFALNKNVTAFYPRLAPT
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA