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The map label for this gene is cisA [H]

Identifier: 154244234

GI number: 154244234

Start: 277976

End: 279631

Strand: Direct

Name: cisA [H]

Synonym: Xaut_0277

Alternate gene names: 154244234

Gene position: 277976-279631 (Clockwise)

Preceding gene: 154244233

Following gene: 154244236

Centisome position: 5.24

GC content: 65.22

Gene sequence:

>1656_bases
ATGACCGTACCGCAGCGCGCCGCTCTCTATCTGCGCGTCTCGACGGCGCGGCAGGCCGAGCATGATGTCTCCATCCCCGA
TCAGAAGCGCCAGGGCGAAGCCTATTGCGCCTCGCGCGGCTATCAGCTCGTCGAAACCTATGTGGAGCCGGGCGCATCGG
CGACCAACGACCGTCGCCCCGAGTTCCAGCGTATGATCGAGGCGGGCACGTCGAAGCCCGCGCCCTTCGATGTGGTCGTG
GTGCACTCGTTCAGCCGTTTCTTCCGCGACCATTTCGAGCTGGAGTTCTATGTCAGGAAGCTGGCGAAGAACGGGGTGAA
GCTCGTCTCGATCACGCAGGAGATGGGCGACGATCCGATGCACGTCATGATGCGGCAGATCATGGCGCTGTTCGACGAAT
ATCAGTCGAAGGAAAACGCCAAGCACGTCATCCGCGCCTTGAAGGAGAATGCACGGCAAGGTTTCTGGAACGGTTCGCTG
CCGCCCATCGGCTACCGCGTCGTCGCGGCCGAGCAGCGCGGCGCGAAGACCAAGAAGAAGCTGGAGATCGACCCGCTCCA
CGCGGAGACGGTGCGGCTTATCTACCGGCTTGCGCTCCACGGCGATGGCGACCACGGCCAGATGGGCGTGAAGAACATCG
TCAGCCATCTGAACCGCAACCGCATCTTCACCCGCGACGGCGGGCGCTGGGGCATCGGCCAAGTCCACCGCATCCTGACC
CGCCGCACCTACATGGGCGAGCACGAGTTCAACAAACGGTCCAAGAGCAAGGAATTGAAACCCATCAGCGAGATCGTCGT
CGTGCCGGTGCCGCCAATCATCGACCGAGAGACCTTTGACGCGGTGCAGGCGCTGCTCAAGGCCCGCCATCCCGCAGTGA
CGCCATCCGCCGTCATCAGCGGCCCAACCCTTCTTACCGGCCTGATCCACTGCGCCAAGTGCGGTGGGGCCATGACCATC
CGCACCGGCAAGGGCGGGCGCTATCGGTATTACGCCTGCTCCATGAAAGCCCGGCAGGGGCCGACCGCTTGCGAGGGCAT
GGCGGTGCCGATGGACAAGCTGGACGATCTGGTCGCCAGCCATCTCGAAGACCGTCTGCTCCAGCCCGAGCGGCTGGAAA
CGATCCTCGCCGCCGTGCTCGACCGGAGGCAGGAGCGCAGCGAGCGTCAGCGCGAGCACATTGCCGAACTGAACAAGCGA
GCGGCAGAGTCCGAAGCGCGCCTCAAGCGGCTCTACGACGCTATCGAGGCGGGCGTCGCCGACCTGGACGACCCGGCGCT
CAAGGACCGCATCGACGGCCTCAAGGCCATCCGCGATCAGGCGAAGGCCGATGCCGAGCGCGCCCAGGCCATGCTCCAGA
ACTCAGGGAGCAAGGCGGTCACGCCGCAGATGCTCAGCAAGTTTGCCCGCGCCGCGCGCCAGCGCATCCGGCTTGAAGGC
GGCGGCTACCGCCGCGACCATCTCCGCGCGCTCGCCCAGCGCGTCGAGGTCGCCGATGGCGAGGTCCGCATCATGGGATC
GAAGTCTCGGCTGCTTCAGGCGCTTGTGGCGAACGGTAGCGTAAACTCAGTGCCCATTCAGGGACTGAAGTGGCGGACCG
GGCGGGATTCGAACAAATACGCCGGGACCATCTGTCGCCCTTCGGCTGCGCCATGA

Upstream 100 bases:

>100_bases
CGCGAGCGGATCAAGCGCGCACGCCCGTTGCAGAGCGCGGCCTGAGTCATGCGACCTCACCGCTCGGGCCGGAACAACAG
TTGAGAAGGAGGCATTGCCC

Downstream 100 bases:

>100_bases
CCATGTCGGCTTTTTGGACGGTCCGCCACGCAGCTTCTATGTCCGATATGGGCGCGCAAAGCGGACGTCGCTGCGGGGCA
GTCGAGGGGCAGCTATGGGT

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTVPQRAALYLRVSTARQAEHDVSIPDQKRQGEAYCASRGYQLVETYVEPGASATNDRRPEFQRMIEAGTSKPAPFDVVV
VHSFSRFFRDHFELEFYVRKLAKNGVKLVSITQEMGDDPMHVMMRQIMALFDEYQSKENAKHVIRALKENARQGFWNGSL
PPIGYRVVAAEQRGAKTKKKLEIDPLHAETVRLIYRLALHGDGDHGQMGVKNIVSHLNRNRIFTRDGGRWGIGQVHRILT
RRTYMGEHEFNKRSKSKELKPISEIVVVPVPPIIDRETFDAVQALLKARHPAVTPSAVISGPTLLTGLIHCAKCGGAMTI
RTGKGGRYRYYACSMKARQGPTACEGMAVPMDKLDDLVASHLEDRLLQPERLETILAAVLDRRQERSERQREHIAELNKR
AAESEARLKRLYDAIEAGVADLDDPALKDRIDGLKAIRDQAKADAERAQAMLQNSGSKAVTPQMLSKFARAARQRIRLEG
GGYRRDHLRALAQRVEVADGEVRIMGSKSRLLQALVANGSVNSVPIQGLKWRTGRDSNKYAGTICRPSAAP

Sequences:

>Translated_551_residues
MTVPQRAALYLRVSTARQAEHDVSIPDQKRQGEAYCASRGYQLVETYVEPGASATNDRRPEFQRMIEAGTSKPAPFDVVV
VHSFSRFFRDHFELEFYVRKLAKNGVKLVSITQEMGDDPMHVMMRQIMALFDEYQSKENAKHVIRALKENARQGFWNGSL
PPIGYRVVAAEQRGAKTKKKLEIDPLHAETVRLIYRLALHGDGDHGQMGVKNIVSHLNRNRIFTRDGGRWGIGQVHRILT
RRTYMGEHEFNKRSKSKELKPISEIVVVPVPPIIDRETFDAVQALLKARHPAVTPSAVISGPTLLTGLIHCAKCGGAMTI
RTGKGGRYRYYACSMKARQGPTACEGMAVPMDKLDDLVASHLEDRLLQPERLETILAAVLDRRQERSERQREHIAELNKR
AAESEARLKRLYDAIEAGVADLDDPALKDRIDGLKAIRDQAKADAERAQAMLQNSGSKAVTPQMLSKFARAARQRIRLEG
GGYRRDHLRALAQRVEVADGEVRIMGSKSRLLQALVANGSVNSVPIQGLKWRTGRDSNKYAGTICRPSAAP
>Mature_550_residues
TVPQRAALYLRVSTARQAEHDVSIPDQKRQGEAYCASRGYQLVETYVEPGASATNDRRPEFQRMIEAGTSKPAPFDVVVV
HSFSRFFRDHFELEFYVRKLAKNGVKLVSITQEMGDDPMHVMMRQIMALFDEYQSKENAKHVIRALKENARQGFWNGSLP
PIGYRVVAAEQRGAKTKKKLEIDPLHAETVRLIYRLALHGDGDHGQMGVKNIVSHLNRNRIFTRDGGRWGIGQVHRILTR
RTYMGEHEFNKRSKSKELKPISEIVVVPVPPIIDRETFDAVQALLKARHPAVTPSAVISGPTLLTGLIHCAKCGGAMTIR
TGKGGRYRYYACSMKARQGPTACEGMAVPMDKLDDLVASHLEDRLLQPERLETILAAVLDRRQERSERQREHIAELNKRA
AESEARLKRLYDAIEAGVADLDDPALKDRIDGLKAIRDQAKADAERAQAMLQNSGSKAVTPQMLSKFARAARQRIRLEGG
GYRRDHLRALAQRVEVADGEVRIMGSKSRLLQALVANGSVNSVPIQGLKWRTGRDSNKYAGTICRPSAAP

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 61664; Mature: 61532

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPQRAALYLRVSTARQAEHDVSIPDQKRQGEAYCASRGYQLVETYVEPGASATNDRRP
CCCCCCEEEEEEEHHHHHCCCCCCCCCCHHCCCHHHHHCCHHHHHHHHCCCCCCCCCCCH
EFQRMIEAGTSKPAPFDVVVVHSFSRFFRDHFELEFYVRKLAKNGVKLVSITQEMGDDPM
HHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCHH
HVMMRQIMALFDEYQSKENAKHVIRALKENARQGFWNGSLPPIGYRVVAAEQRGAKTKKK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCCCCHHC
LEIDPLHAETVRLIYRLALHGDGDHGQMGVKNIVSHLNRNRIFTRDGGRWGIGQVHRILT
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
RRTYMGEHEFNKRSKSKELKPISEIVVVPVPPIIDRETFDAVQALLKARHPAVTPSAVIS
HHHHCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHC
GPTLLTGLIHCAKCGGAMTIRTGKGGRYRYYACSMKARQGPTACEGMAVPMDKLDDLVAS
CHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
HLEDRLLQPERLETILAAVLDRRQERSERQREHIAELNKRAAESEARLKRLYDAIEAGVA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLDDPALKDRIDGLKAIRDQAKADAERAQAMLQNSGSKAVTPQMLSKFARAARQRIRLEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCC
GGYRRDHLRALAQRVEVADGEVRIMGSKSRLLQALVANGSVNSVPIQGLKWRTGRDSNKY
CCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
AGTICRPSAAP
CCEECCCCCCC
>Mature Secondary Structure 
TVPQRAALYLRVSTARQAEHDVSIPDQKRQGEAYCASRGYQLVETYVEPGASATNDRRP
CCCCCEEEEEEEHHHHHCCCCCCCCCCHHCCCHHHHHCCHHHHHHHHCCCCCCCCCCCH
EFQRMIEAGTSKPAPFDVVVVHSFSRFFRDHFELEFYVRKLAKNGVKLVSITQEMGDDPM
HHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCHH
HVMMRQIMALFDEYQSKENAKHVIRALKENARQGFWNGSLPPIGYRVVAAEQRGAKTKKK
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHCCCCCHHC
LEIDPLHAETVRLIYRLALHGDGDHGQMGVKNIVSHLNRNRIFTRDGGRWGIGQVHRILT
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
RRTYMGEHEFNKRSKSKELKPISEIVVVPVPPIIDRETFDAVQALLKARHPAVTPSAVIS
HHHHCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHC
GPTLLTGLIHCAKCGGAMTIRTGKGGRYRYYACSMKARQGPTACEGMAVPMDKLDDLVAS
CHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHH
HLEDRLLQPERLETILAAVLDRRQERSERQREHIAELNKRAAESEARLKRLYDAIEAGVA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DLDDPALKDRIDGLKAIRDQAKADAERAQAMLQNSGSKAVTPQMLSKFARAARQRIRLEG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEECCC
GGYRRDHLRALAQRVEVADGEVRIMGSKSRLLQALVANGSVNSVPIQGLKWRTGRDSNKY
CCCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCC
AGTICRPSAAP
CCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]