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The map label for this gene is xthA [H]

Identifier: 154244152

GI number: 154244152

Start: 194546

End: 195418

Strand: Direct

Name: xthA [H]

Synonym: Xaut_0194

Alternate gene names: 154244152

Gene position: 194546-195418 (Clockwise)

Preceding gene: 154244151

Following gene: 154244154

Centisome position: 3.66

GC content: 67.12

Gene sequence:

>873_bases
GTGCCGGGGTGTAAGGCTCGCGGCATCCTTCCTTTTCAGTCCGCCATTGCCTTGCCCCTCACAATCTGCACCTGGAACAT
CAATTCCGTCCGCATTCGTCTCGACATCGTCCGTGCGGCGGTGGAGACGCTGCGGCCCGACATCCTGTGCCTCCAAGAAA
CGAAGTGCCAGGACGACAAGTTTCCCCTCGCCGCCATTCGCGACATGGGCTTTCCCCACATCGCCCTCAACGGGCAGAAG
GGCTGGCACGGGGTCGCGACCTTCTCGCGCCGGCCGTTCGTCTCGGTGGAGAAGGGCGATTTCTGCGGCAAGGGCGACGC
GCGCCACGTGGCGGTGACCCTCGCCCCCGAGCACGCGCTGGGCAAGCCGCTGACGGTGCATAATTTCTACGTGCCCGCCG
GTGGCGACATCCCGGATCCGGAGCTGAACCCCAAGTTCGCCCACAAGCTCGCCTTCCTCGACGAGGTGACGGCCTGGCCG
CCGCTTGCCGCAGCAGCGGCGGATGACGGGCGCCACGCGGTGGTGGTGGGCGACCTCAACATCGCCCCGCTGGAAACCGA
CGTGTGGGGCCACAAGCAGCTGCTCAACGTGGTCAGCCACACCCCCGTCGAGGTGGAAAGGCTGAACCGCTTCCAGGCCT
CCGGCCCCTGGGTCGACGTGATGCGCACCTTCGTGCCGCCCAAGGACAAGCTCTTCACCTGGTGGAGCTACCGCTCGCCC
GACTACACCGCCAACGACCGCGGCCGCAGGCTGGACCATGTCTGGGCCAGCCAGAGTCTCGCGCCCTCCGCGCAGGCCAT
GACGGTGCTGAAGGACGCACGCGGCTGGGAAAAGCCCTCCGACCACGTGCCGGTGTCCCTCACCTTCGGCTGA

Upstream 100 bases:

>100_bases
CGACTTCGTTCGCACACCGGTTTCACGCGCCTTTTCGCGTGGCTTTTTCGCGCGACTTGGCCGCAGCCGTTCCTTGGCAG
GCGCCGCTTGCCTGCCGGCG

Downstream 100 bases:

>100_bases
GGCAGCGGCGCCGCGCAGCCGGCGCGGTCGCCCCGGCTTCCGGCGTCAGGGTTTCGGCGCGAGATCGAGCAGCGGCGCGC
CGAGGTTGGGCAGCCCCAGG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MPGCKARGILPFQSAIALPLTICTWNINSVRIRLDIVRAAVETLRPDILCLQETKCQDDKFPLAAIRDMGFPHIALNGQK
GWHGVATFSRRPFVSVEKGDFCGKGDARHVAVTLAPEHALGKPLTVHNFYVPAGGDIPDPELNPKFAHKLAFLDEVTAWP
PLAAAAADDGRHAVVVGDLNIAPLETDVWGHKQLLNVVSHTPVEVERLNRFQASGPWVDVMRTFVPPKDKLFTWWSYRSP
DYTANDRGRRLDHVWASQSLAPSAQAMTVLKDARGWEKPSDHVPVSLTFG

Sequences:

>Translated_290_residues
MPGCKARGILPFQSAIALPLTICTWNINSVRIRLDIVRAAVETLRPDILCLQETKCQDDKFPLAAIRDMGFPHIALNGQK
GWHGVATFSRRPFVSVEKGDFCGKGDARHVAVTLAPEHALGKPLTVHNFYVPAGGDIPDPELNPKFAHKLAFLDEVTAWP
PLAAAAADDGRHAVVVGDLNIAPLETDVWGHKQLLNVVSHTPVEVERLNRFQASGPWVDVMRTFVPPKDKLFTWWSYRSP
DYTANDRGRRLDHVWASQSLAPSAQAMTVLKDARGWEKPSDHVPVSLTFG
>Mature_289_residues
PGCKARGILPFQSAIALPLTICTWNINSVRIRLDIVRAAVETLRPDILCLQETKCQDDKFPLAAIRDMGFPHIALNGQKG
WHGVATFSRRPFVSVEKGDFCGKGDARHVAVTLAPEHALGKPLTVHNFYVPAGGDIPDPELNPKFAHKLAFLDEVTAWPP
LAAAAADDGRHAVVVGDLNIAPLETDVWGHKQLLNVVSHTPVEVERLNRFQASGPWVDVMRTFVPPKDKLFTWWSYRSPD
YTANDRGRRLDHVWASQSLAPSAQAMTVLKDARGWEKPSDHVPVSLTFG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=276, Percent_Identity=32.9710144927536, Blast_Score=122, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221330655, Length=292, Percent_Identity=27.3972602739726, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17136678, Length=292, Percent_Identity=27.3972602739726, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32043; Mature: 31912

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGCKARGILPFQSAIALPLTICTWNINSVRIRLDIVRAAVETLRPDILCLQETKCQDDK
CCCCCCCCCCCHHHHHCCEEEEEEECCCEEEEEHHHHHHHHHHHCCCEEEEECCCCCCCC
FPLAAIRDMGFPHIALNGQKGWHGVATFSRRPFVSVEKGDFCGKGDARHVAVTLAPEHAL
CCHHHHHHCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCCCCCCCCEEEEEECCHHHC
GKPLTVHNFYVPAGGDIPDPELNPKFAHKLAFLDEVTAWPPLAAAAADDGRHAVVVGDLN
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCE
IAPLETDVWGHKQLLNVVSHTPVEVERLNRFQASGPWVDVMRTFVPPKDKLFTWWSYRSP
ECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEECCCC
DYTANDRGRRLDHVWASQSLAPSAQAMTVLKDARGWEKPSDHVPVSLTFG
CCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
PGCKARGILPFQSAIALPLTICTWNINSVRIRLDIVRAAVETLRPDILCLQETKCQDDK
CCCCCCCCCCHHHHHCCEEEEEEECCCEEEEEHHHHHHHHHHHCCCEEEEECCCCCCCC
FPLAAIRDMGFPHIALNGQKGWHGVATFSRRPFVSVEKGDFCGKGDARHVAVTLAPEHAL
CCHHHHHHCCCCEEEECCCCCCCCEEECCCCCEEEEECCCCCCCCCCCEEEEEECCHHHC
GKPLTVHNFYVPAGGDIPDPELNPKFAHKLAFLDEVTAWPPLAAAAADDGRHAVVVGDLN
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCE
IAPLETDVWGHKQLLNVVSHTPVEVERLNRFQASGPWVDVMRTFVPPKDKLFTWWSYRSP
ECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEECCCC
DYTANDRGRRLDHVWASQSLAPSAQAMTVLKDARGWEKPSDHVPVSLTFG
CCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]