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The map label for this gene is hycG [H]

Identifier: 154244129

GI number: 154244129

Start: 168444

End: 168956

Strand: Direct

Name: hycG [H]

Synonym: Xaut_0171

Alternate gene names: 154244129

Gene position: 168444-168956 (Clockwise)

Preceding gene: 154244128

Following gene: 154244131

Centisome position: 3.17

GC content: 70.96

Gene sequence:

>513_bases
ATGCGCAAGCTTCTCCTCCAAGGGCTTTTGAAAGCCCCGCTCACGGAGGCCGCCCCCGAGGACGCGGCGGTGGAAAAGCT
CGCCGCCGAGCTGAAGGCGGCGGCGCGCGCGCGGCTCGGGCGCAGCCTGTCCATCCGCGAGGTGGATGCCGGCTCGTGCA
ACGGCTGCGAGCTGGAAATCCATGCGCTCGGCAATGCCTTCTACGACCTGGAGCGCTTCGGCCTGCATGTGGTCGCCTCG
CCCCGCCATGCGGACGTGCTGCTGGTGACCGGGCCGGTGGCGAAGAACATGGCCGAGGCGCTGAAGCGCACCTATGACGC
GACGCCGTCGCCGAAATGGGTGGTGGCGGCCGGCGATTGCGCCATCAACGGCGGCATCTTCGCCGGCAGCTCTGCCTGCG
CTGGCGGCGTGGAGGCCATCGTGCCGGTGGACCTGAGGATCCCCGGCTGCCCGCCGACGCCGGTGGCGCTCCTGAGCGGC
CTCCTCGCTTTGCTGCGGGCCGGCAAGGGCTGA

Upstream 100 bases:

>100_bases
GCTGGAAACCGCCATCGAGGGCAACATCGTCGCCGACTTCCCGCTCTGCAACAAATCCTTCAACTGCTCCTATTCGGGCG
TGGATCTTTAAGGGGCGGCC

Downstream 100 bases:

>100_bases
GGCCGAGCCGCGTTCAGCCGGCGCGCAGGCCGTCGTTGGCGTCGGCATCGGCGCCGGGCGGACGGGCGTCGATGGAGCGC
AGCAGGGTGGCGCGCGCGGT

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MRKLLLQGLLKAPLTEAAPEDAAVEKLAAELKAAARARLGRSLSIREVDAGSCNGCELEIHALGNAFYDLERFGLHVVAS
PRHADVLLVTGPVAKNMAEALKRTYDATPSPKWVVAAGDCAINGGIFAGSSACAGGVEAIVPVDLRIPGCPPTPVALLSG
LLALLRAGKG

Sequences:

>Translated_170_residues
MRKLLLQGLLKAPLTEAAPEDAAVEKLAAELKAAARARLGRSLSIREVDAGSCNGCELEIHALGNAFYDLERFGLHVVAS
PRHADVLLVTGPVAKNMAEALKRTYDATPSPKWVVAAGDCAINGGIFAGSSACAGGVEAIVPVDLRIPGCPPTPVALLSG
LLALLRAGKG
>Mature_170_residues
MRKLLLQGLLKAPLTEAAPEDAAVEKLAAELKAAARARLGRSLSIREVDAGSCNGCELEIHALGNAFYDLERFGLHVVAS
PRHADVLLVTGPVAKNMAEALKRTYDATPSPKWVVAAGDCAINGGIFAGSSACAGGVEAIVPVDLRIPGCPPTPVALLSG
LLALLRAGKG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=114, Percent_Identity=45.6140350877193, Blast_Score=111, Evalue=4e-25,
Organism=Escherichia coli, GI1789074, Length=143, Percent_Identity=45.4545454545455, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1788834, Length=148, Percent_Identity=41.2162162162162, Blast_Score=126, Evalue=6e-31,
Organism=Escherichia coli, GI1788624, Length=163, Percent_Identity=36.1963190184049, Blast_Score=97, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17509685, Length=114, Percent_Identity=42.1052631578947, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI18859983, Length=131, Percent_Identity=41.2213740458015, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24642371, Length=131, Percent_Identity=41.2213740458015, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24651058, Length=114, Percent_Identity=42.9824561403509, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 17487; Mature: 17487

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLLQGLLKAPLTEAAPEDAAVEKLAAELKAAARARLGRSLSIREVDAGSCNGCELEI
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEE
HALGNAFYDLERFGLHVVASPRHADVLLVTGPVAKNMAEALKRTYDATPSPKWVVAAGDC
EECCCHHHHHHHCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCE
AINGGIFAGSSACAGGVEAIVPVDLRIPGCPPTPVALLSGLLALLRAGKG
EECCCEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKLLLQGLLKAPLTEAAPEDAAVEKLAAELKAAARARLGRSLSIREVDAGSCNGCELEI
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEE
HALGNAFYDLERFGLHVVASPRHADVLLVTGPVAKNMAEALKRTYDATPSPKWVVAAGDC
EECCCHHHHHHHCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCE
AINGGIFAGSSACAGGVEAIVPVDLRIPGCPPTPVALLSGLLALLRAGKG
EECCCEEECCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]