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The map label for this gene is hycE [H]

Identifier: 154244128

GI number: 154244128

Start: 166929

End: 168434

Strand: Direct

Name: hycE [H]

Synonym: Xaut_0170

Alternate gene names: 154244128

Gene position: 166929-168434 (Clockwise)

Preceding gene: 154244127

Following gene: 154244129

Centisome position: 3.14

GC content: 71.51

Gene sequence:

>1506_bases
ATGCAAGCCTTCGCACAGCTTCTGGAAAACGGCCGCGCCGGTCCCTGCCGGCCCTTTCCGCGCGCCGAGGTGAGTGCAGA
GCAGTGGACCGCCCTCGCCGGGCATCTGGCGGCGGGCGAGCTGGTGCTCATGGGCCTATGGGGCGATGCGGGCGCCGTGC
ATATGGCGCTGCTGGACGAGGCATCCGGCCGGCTGGCCGTGGCGACCTTTGCCTGTGTTGGCGGCACCTTCCCCTCGGTG
GCGCGCCATCACCCGCCGGCGGCGCGGCTGGAGCGCACCGTGCGCGACCTCTACGGCCTCGTGCCCGAGGGCGCACCCGA
CGAGCGCCCGTGGCTCGATCACGGCCGCTGGGGCACCCGCCAACCGCTGGGGGCGAACCTGCCGGCGGAGCCTGAGGGCG
ATGCCTATCTCTTCCTCGCGGCGGAAGGGGAGGGGCTGCACCAGATCCCGGTGGGGCCGGTGCATGCGGGCATCATCGAG
CCCGGCCATTTCCGCTTCTCCTGCTACGGCGAGACGGTGGTGCGGCTGGAAGCCCGCCTCGGCTATGTGCACAAGGGCAT
CGAGCGCCTCATGGCCGGCGCGGACGTGGCGCAGGCGGCGCGGCTGGCCGGGCGCCAGTCCGGCGACAGCACGGTGGCCT
ATGCGCTCGCCTTCAGCCGCGCGGTGGAAGCCGCGCGTGGCGTTGGAGTGCCAGCGCGGGCTCATGCCCTGCGCGGCGTG
ATGGCGGAGCTGGAGCGCCTTGCCAACCATCTCGGCGACATCGGCGCCATCTGCAACGATGCCGCCTTCGCGCTGATGCT
GGCCCATTGTGGCGTGCTGCGCGAGAAGGTGCTGCGGGTCTGCGCCCGCGCCTTCGGCCATCGGCTGATGATGGATGGCG
TCATCCCCGGCGGGGTGGCGGGGGATCTGGCGCGGGACGGTGTCTCGGCCATCCGCGTGCTGGTGTCCGAGGTGCGCGCC
GCCTTCCCGGCGCTGGTGGAGCTTTACGACAACACCGCCTCGCTGCAGGACCGCACCGTGGGCACTGGCTTCACCCGGCC
GGATTATGTCCGCCAGTTCGGCGCCGGCGGCTTCGTGGGCCGCGCCTCCGGCCGGCCCTTCGACGCACGGCGGGCGCTCG
CCTATCCGCCGTATGATCGCCTGCCGTTCGAGGTGGTGGTGCGGTCCGAGGGTGACGTGAACGCGCGCATCTGGGTGCGC
ATCCGCGAGGTGGAGCAGAGCCTTGGCCTCATCGTGCGCCTGCTCGACACCCTGCCGGACGGTCCGGTCGCGGTGGAGGT
TCCGGCGGGTCCCGGAGAGGGCCTCGCGGTGGTGGAAGGCTTCCGCGGCGACGTGCTGGCCTTCGTGGCGCTGGAGGCGG
ACGGGCGGGTGCGGCGTGCGCATCTGCGTGACCCGTCCTGGTTCCAGTGGCCGCTGCTGGAAACCGCCATCGAGGGCAAC
ATCGTCGCCGACTTCCCGCTCTGCAACAAATCCTTCAACTGCTCCTATTCGGGCGTGGATCTTTAA

Upstream 100 bases:

>100_bases
GGTGCATCTTGCCCTGGTGCTGGTGGCCGGGCTCTATCTGCCGCCGCCGCTGGTGGCGTGGTTCCGGCACGTGGCCGGGC
TTCTGGGCTAGGGGCGCGCC

Downstream 100 bases:

>100_bases
GGGGCGGCCATGCGCAAGCTTCTCCTCCAAGGGCTTTTGAAAGCCCCGCTCACGGAGGCCGCCCCCGAGGACGCGGCGGT
GGAAAAGCTCGCCGCCGAGC

Product: NADH-ubiquinone oxidoreductase chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MQAFAQLLENGRAGPCRPFPRAEVSAEQWTALAGHLAAGELVLMGLWGDAGAVHMALLDEASGRLAVATFACVGGTFPSV
ARHHPPAARLERTVRDLYGLVPEGAPDERPWLDHGRWGTRQPLGANLPAEPEGDAYLFLAAEGEGLHQIPVGPVHAGIIE
PGHFRFSCYGETVVRLEARLGYVHKGIERLMAGADVAQAARLAGRQSGDSTVAYALAFSRAVEAARGVGVPARAHALRGV
MAELERLANHLGDIGAICNDAAFALMLAHCGVLREKVLRVCARAFGHRLMMDGVIPGGVAGDLARDGVSAIRVLVSEVRA
AFPALVELYDNTASLQDRTVGTGFTRPDYVRQFGAGGFVGRASGRPFDARRALAYPPYDRLPFEVVVRSEGDVNARIWVR
IREVEQSLGLIVRLLDTLPDGPVAVEVPAGPGEGLAVVEGFRGDVLAFVALEADGRVRRAHLRDPSWFQWPLLETAIEGN
IVADFPLCNKSFNCSYSGVDL

Sequences:

>Translated_501_residues
MQAFAQLLENGRAGPCRPFPRAEVSAEQWTALAGHLAAGELVLMGLWGDAGAVHMALLDEASGRLAVATFACVGGTFPSV
ARHHPPAARLERTVRDLYGLVPEGAPDERPWLDHGRWGTRQPLGANLPAEPEGDAYLFLAAEGEGLHQIPVGPVHAGIIE
PGHFRFSCYGETVVRLEARLGYVHKGIERLMAGADVAQAARLAGRQSGDSTVAYALAFSRAVEAARGVGVPARAHALRGV
MAELERLANHLGDIGAICNDAAFALMLAHCGVLREKVLRVCARAFGHRLMMDGVIPGGVAGDLARDGVSAIRVLVSEVRA
AFPALVELYDNTASLQDRTVGTGFTRPDYVRQFGAGGFVGRASGRPFDARRALAYPPYDRLPFEVVVRSEGDVNARIWVR
IREVEQSLGLIVRLLDTLPDGPVAVEVPAGPGEGLAVVEGFRGDVLAFVALEADGRVRRAHLRDPSWFQWPLLETAIEGN
IVADFPLCNKSFNCSYSGVDL
>Mature_501_residues
MQAFAQLLENGRAGPCRPFPRAEVSAEQWTALAGHLAAGELVLMGLWGDAGAVHMALLDEASGRLAVATFACVGGTFPSV
ARHHPPAARLERTVRDLYGLVPEGAPDERPWLDHGRWGTRQPLGANLPAEPEGDAYLFLAAEGEGLHQIPVGPVHAGIIE
PGHFRFSCYGETVVRLEARLGYVHKGIERLMAGADVAQAARLAGRQSGDSTVAYALAFSRAVEAARGVGVPARAHALRGV
MAELERLANHLGDIGAICNDAAFALMLAHCGVLREKVLRVCARAFGHRLMMDGVIPGGVAGDLARDGVSAIRVLVSEVRA
AFPALVELYDNTASLQDRTVGTGFTRPDYVRQFGAGGFVGRASGRPFDARRALAYPPYDRLPFEVVVRSEGDVNARIWVR
IREVEQSLGLIVRLLDTLPDGPVAVEVPAGPGEGLAVVEGFRGDVLAFVALEADGRVRRAHLRDPSWFQWPLLETAIEGN
IVADFPLCNKSFNCSYSGVDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=346, Percent_Identity=25.1445086705202, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4758786, Length=346, Percent_Identity=25.1445086705202, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1789076, Length=438, Percent_Identity=33.1050228310502, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788832, Length=463, Percent_Identity=31.1015118790497, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI145693162, Length=514, Percent_Identity=22.568093385214, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17568379, Length=257, Percent_Identity=27.6264591439689, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17555284, Length=254, Percent_Identity=27.9527559055118, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221459469, Length=346, Percent_Identity=27.4566473988439, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24638644, Length=362, Percent_Identity=22.9281767955801, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 53600; Mature: 53600

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFAQLLENGRAGPCRPFPRAEVSAEQWTALAGHLAAGELVLMGLWGDAGAVHMALLDE
CHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEHHC
ASGRLAVATFACVGGTFPSVARHHPPAARLERTVRDLYGLVPEGAPDERPWLDHGRWGTR
CCCCEEEEEHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QPLGANLPAEPEGDAYLFLAAEGEGLHQIPVGPVHAGIIEPGHFRFSCYGETVVRLEARL
CCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
GYVHKGIERLMAGADVAQAARLAGRQSGDSTVAYALAFSRAVEAARGVGVPARAHALRGV
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MAELERLANHLGDIGAICNDAAFALMLAHCGVLREKVLRVCARAFGHRLMMDGVIPGGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCH
GDLARDGVSAIRVLVSEVRAAFPALVELYDNTASLQDRTVGTGFTRPDYVRQFGAGGFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEE
RASGRPFDARRALAYPPYDRLPFEVVVRSEGDVNARIWVRIREVEQSLGLIVRLLDTLPD
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
GPVAVEVPAGPGEGLAVVEGFRGDVLAFVALEADGRVRRAHLRDPSWFQWPLLETAIEGN
CCEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCCEEEHHCCCCCCEECHHHHHHHCCC
IVADFPLCNKSFNCSYSGVDL
EEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAFAQLLENGRAGPCRPFPRAEVSAEQWTALAGHLAAGELVLMGLWGDAGAVHMALLDE
CHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEHHC
ASGRLAVATFACVGGTFPSVARHHPPAARLERTVRDLYGLVPEGAPDERPWLDHGRWGTR
CCCCEEEEEHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QPLGANLPAEPEGDAYLFLAAEGEGLHQIPVGPVHAGIIEPGHFRFSCYGETVVRLEARL
CCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHH
GYVHKGIERLMAGADVAQAARLAGRQSGDSTVAYALAFSRAVEAARGVGVPARAHALRGV
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
MAELERLANHLGDIGAICNDAAFALMLAHCGVLREKVLRVCARAFGHRLMMDGVIPGGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCH
GDLARDGVSAIRVLVSEVRAAFPALVELYDNTASLQDRTVGTGFTRPDYVRQFGAGGFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEE
RASGRPFDARRALAYPPYDRLPFEVVVRSEGDVNARIWVRIREVEQSLGLIVRLLDTLPD
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
GPVAVEVPAGPGEGLAVVEGFRGDVLAFVALEADGRVRRAHLRDPSWFQWPLLETAIEGN
CCEEEEECCCCCCCEEEEECCCCCEEEEEEECCCCCEEEHHCCCCCCEECHHHHHHHCCC
IVADFPLCNKSFNCSYSGVDL
EEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]