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The map label for this gene is sucB [H]

Identifier: 154244116

GI number: 154244116

Start: 154367

End: 155596

Strand: Direct

Name: sucB [H]

Synonym: Xaut_0158

Alternate gene names: 154244116

Gene position: 154367-155596 (Clockwise)

Preceding gene: 154244115

Following gene: 154244117

Centisome position: 2.91

GC content: 69.43

Gene sequence:

>1230_bases
ATGACCACCGAGATCCGCGTACCGACCCTGGGCGAGTCCGTTACCGAAGCGACCATCGGCAAGTGGTTCAAGAAGCCCGG
CGATACCGTCAAGGCCGATGAGCCGCTGGTGGAACTGGAGACCGACAAGGTCACCGTCGAGGTTCCCGCGCCGGCTGCCG
GCGTGCTGGCCGAGATCGTCGCCAAGGACGGCGACACCGTGGGCGTCGGCGCCCTGCTCGGCTCCATCGGCGCCGGCTCC
GGCGCTGCTGCCGCGGCTCCCGCTGCCGCGGCTCCCGCGCCCGCCGCTGCCGCCCCTGCGCCGGCCCCGGCTCCCGCCGC
GGCGCCTGCCGCGTCCGGCTCAAGTGTCAATGGCCCGGCCGTGGGCCGCATCGCCGCCGAGAGCGGTGTTGATCCCTCGG
GCGTTGCCGGTTCCGGCAAGGATGGCCGCGTCACCAAGGGTGACATGCTGGCCGCCGTCGCCGCCGGCGCTGCTGCCGCT
CCCGCTGCGGCCCCGATCGCGGTCCGTGCCCCCTCGGCACCCATCGATGCGGTGCGCGAGGAGCGGGTCAAGATGACCAA
GCTGCGCCAGACCATCGCGCGGCGCCTGAAGGACGCCCAGAACACCGCGGCCATGCTCACCACGTTCAACGACGTGGACA
TGAGCGCGGTGATGAGCCTGCGCGCCCAGTTCAAGGATGCGTTCGAGAAGAAGCACGGCACCAAGCTCGGCTTCATGGGC
TTCTTCACCAAGGCGGTCATCGCCGCCCTGAAGGACCTGCCGGCGGTGAACGCCGAGATCGACGGCCAGGACCTCGTCTA
CAAGAACTACTACAACATCGGTATCGCGGTGGGCACCGAGAAGGGCCTGGTGGTTCCGGTGGTGCGCGACGCCGACCAGC
TCTCCGTGGCGGAGATCGAGAAGGCCATCGCCGGCTTCGGCCGCAAGGCGCGTGACGGCAAGCTCGGCATCGAGGACATG
CAGGGCGGCACCTTCACCATCACCAACGGCGGCATCTACGGCTCGCTGATGTCCACCCCGATCCTGAACGCGCCGCAGTC
GGGCATCCTCGGCATGCACCGCATCGAGGAGCGCCCGGTGGCCATCAAGGGCCAGGTGGTGATCCGCCCGATGATGTACC
TCGCCCTCTCCTACGACCACCGCATCGTCGATGGCCGCGAGGCCGTGACCTTCCTGGTGCGGGTGAAGGAGACCCTGGAA
GATCCCGCGCGGCTCGTGCTGGATCTGTGA

Upstream 100 bases:

>100_bases
GCCCATCCTGTCGCTCATTCAGCGCCGGGTGGCTGGCCCGGCGGCTGTTGCATCTTCACCTGCCGATAGTTGAGGGAGGC
CCCGCACAGGGGCGAACGCA

Downstream 100 bases:

>100_bases
TGAGCGGCCGGGAGCTTGTCCGTGAATGGGCAGGCTCCCAGCCTCTCATTGCGGAAGTTTGGCTGTTCGGCAGTCGAGCG
AAAGGCGCGGAGAGGGGCGA

Product: 2-oxoglutarate dehydrogenase, E2 subunit, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTTEIRVPTLGESVTEATIGKWFKKPGDTVKADEPLVELETDKVTVEVPAPAAGVLAEIVAKDGDTVGVGALLGSIGAGS
GAAAAAPAAAAPAPAAAAPAPAPAPAAAPAASGSSVNGPAVGRIAAESGVDPSGVAGSGKDGRVTKGDMLAAVAAGAAAA
PAAAPIAVRAPSAPIDAVREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMSLRAQFKDAFEKKHGTKLGFMG
FFTKAVIAALKDLPAVNAEIDGQDLVYKNYYNIGIAVGTEKGLVVPVVRDADQLSVAEIEKAIAGFGRKARDGKLGIEDM
QGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPVAIKGQVVIRPMMYLALSYDHRIVDGREAVTFLVRVKETLE
DPARLVLDL

Sequences:

>Translated_409_residues
MTTEIRVPTLGESVTEATIGKWFKKPGDTVKADEPLVELETDKVTVEVPAPAAGVLAEIVAKDGDTVGVGALLGSIGAGS
GAAAAAPAAAAPAPAAAAPAPAPAPAAAPAASGSSVNGPAVGRIAAESGVDPSGVAGSGKDGRVTKGDMLAAVAAGAAAA
PAAAPIAVRAPSAPIDAVREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMSLRAQFKDAFEKKHGTKLGFMG
FFTKAVIAALKDLPAVNAEIDGQDLVYKNYYNIGIAVGTEKGLVVPVVRDADQLSVAEIEKAIAGFGRKARDGKLGIEDM
QGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPVAIKGQVVIRPMMYLALSYDHRIVDGREAVTFLVRVKETLE
DPARLVLDL
>Mature_408_residues
TTEIRVPTLGESVTEATIGKWFKKPGDTVKADEPLVELETDKVTVEVPAPAAGVLAEIVAKDGDTVGVGALLGSIGAGSG
AAAAAPAAAAPAPAAAAPAPAPAPAAAPAASGSSVNGPAVGRIAAESGVDPSGVAGSGKDGRVTKGDMLAAVAAGAAAAP
AAAPIAVRAPSAPIDAVREERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMSLRAQFKDAFEKKHGTKLGFMGF
FTKAVIAALKDLPAVNAEIDGQDLVYKNYYNIGIAVGTEKGLVVPVVRDADQLSVAEIEKAIAGFGRKARDGKLGIEDMQ
GGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPVAIKGQVVIRPMMYLALSYDHRIVDGREAVTFLVRVKETLED
PARLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=58.6206896551724, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=29.1954022988506, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI203098753, Length=450, Percent_Identity=28.4444444444444, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=29.1666666666667, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI203098816, Length=450, Percent_Identity=28.4444444444444, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=35.3293413173653, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=50.9803921568627, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=36.2352941176471, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=43.0317848410758, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=30.3854875283447, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=428, Percent_Identity=27.803738317757, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=28.9808917197452, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=407, Percent_Identity=46.1916461916462, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=26.9146608315098, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=61.2334801762115, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=28.8329519450801, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI20129315, Length=445, Percent_Identity=32.3595505617978, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24582497, Length=429, Percent_Identity=32.4009324009324, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 41979; Mature: 41847

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEIRVPTLGESVTEATIGKWFKKPGDTVKADEPLVELETDKVTVEVPAPAAGVLAEIV
CCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHH
AKDGDTVGVGALLGSIGAGSGAAAAAPAAAAPAPAAAAPAPAPAPAAAPAASGSSVNGPA
HCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VGRIAAESGVDPSGVAGSGKDGRVTKGDMLAAVAAGAAAAPAAAPIAVRAPSAPIDAVRE
EEEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
ERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMSLRAQFKDAFEKKHGTKLGFMG
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHH
FFTKAVIAALKDLPAVNAEIDGQDLVYKNYYNIGIAVGTEKGLVVPVVRDADQLSVAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KAIAGFGRKARDGKLGIEDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPV
HHHHHCCCCCCCCCCCCEECCCCEEEEECCCEEEHHHHCCCCCCCCCCCCCHHHHCCCCE
AIKGQVVIRPMMYLALSYDHRIVDGREAVTFLVRVKETLEDPARLVLDL
EEECHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHEECC
>Mature Secondary Structure 
TTEIRVPTLGESVTEATIGKWFKKPGDTVKADEPLVELETDKVTVEVPAPAAGVLAEIV
CCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHH
AKDGDTVGVGALLGSIGAGSGAAAAAPAAAAPAPAAAAPAPAPAPAAAPAASGSSVNGPA
HCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VGRIAAESGVDPSGVAGSGKDGRVTKGDMLAAVAAGAAAAPAAAPIAVRAPSAPIDAVRE
EEEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHH
ERVKMTKLRQTIARRLKDAQNTAAMLTTFNDVDMSAVMSLRAQFKDAFEKKHGTKLGFMG
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHH
FFTKAVIAALKDLPAVNAEIDGQDLVYKNYYNIGIAVGTEKGLVVPVVRDADQLSVAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KAIAGFGRKARDGKLGIEDMQGGTFTITNGGIYGSLMSTPILNAPQSGILGMHRIEERPV
HHHHHCCCCCCCCCCCCEECCCCEEEEECCCEEEHHHHCCCCCCCCCCCCCHHHHCCCCE
AIKGQVVIRPMMYLALSYDHRIVDGREAVTFLVRVKETLEDPARLVLDL
EEECHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]