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The map label for this gene is glpE [H]

Identifier: 154244105

GI number: 154244105

Start: 141529

End: 142320

Strand: Direct

Name: glpE [H]

Synonym: Xaut_0147

Alternate gene names: 154244105

Gene position: 141529-142320 (Clockwise)

Preceding gene: 154244104

Following gene: 154244106

Centisome position: 2.67

GC content: 67.42

Gene sequence:

>792_bases
ATGAGAGAGCGCATTCCCTTCCGCTGCATCGGCGTTGAGGAGGCGGCATCCATCGTCGGCCAAGGCCGGGCCCTGCTGCT
CGACGTGCGCGACGCCAATGCCTATGCCGCCGGCCATGTGGAGGGTGCGCGCAACCTTGCCTATTCCGCGCTCTCGGACG
TGATCGGCACGACCCCGAAGGCGCAGCCGGTGGTGATTTATTGCTACCACGGCAATGCCAGCCGCGAATATGCTCAGACC
CTGTCGGACTTCGGCTTCGCTGCCGTGTTCAGCGTGGATGGTGGCTATGAGGCGTGGCGGGCCTTCGAAAGCGTGCCCGC
GCCAACGGCGCCACTCGATGCGGCCCTGTCCAGCTGGCTGGTTGACCACGGATTTCCCGCCGATGGCGTGAACGCGACGG
CGGAAAATGGCATGACCCCGCTCATGAAGGCCTCCCAGGAGGGGCTCGCGGACGTGGTGGCGGCCCTCATTGCCGCCGGC
GCGACGCTGGACGCCCGCAATGGGGATGGCAACACGGCCCTGTGGCTCGCCTGCGTCGGCAATCACCCGCACATCATCGA
CGCGCTGGTGGATGCCGGCATCACCATCGACAGCCTCAACGACACCGGCGCCAGTTCGTTGATGTATGCCGCCTCCGCCG
GCAAGGCCGAGGCGTTGGAAAAGCTGCTCGCCCGTGGCGCCGATCCGCGCCTGGAGACGCAGGACGGCTTCTGCGCGCTG
GATCTTGCGGCCAATCTCGAATGCCTCAAGCTGCTGCGCGCCGCCACACGGCGCGAGGCCGCGCCGGCGTAA

Upstream 100 bases:

>100_bases
TCTATCATTTCACGGTGGGGCGCCCCGCCATCGACACCCGCATTACCACCGAGCCGGCCTATCCGGGCCGCTCGGCCTGA
GGGACCTTCAGGAGCACGCC

Downstream 100 bases:

>100_bases
GGGCTGCGGTGCTGCGGGTTTCCCGGCAAGCGCTTCTTTGTCCCGTAGACCATCCCGCCCGGGCGTTATAAGCCTGCCAG
CTGTATCGGATCCGGCATAG

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MRERIPFRCIGVEEAASIVGQGRALLLDVRDANAYAAGHVEGARNLAYSALSDVIGTTPKAQPVVIYCYHGNASREYAQT
LSDFGFAAVFSVDGGYEAWRAFESVPAPTAPLDAALSSWLVDHGFPADGVNATAENGMTPLMKASQEGLADVVAALIAAG
ATLDARNGDGNTALWLACVGNHPHIIDALVDAGITIDSLNDTGASSLMYAASAGKAEALEKLLARGADPRLETQDGFCAL
DLAANLECLKLLRAATRREAAPA

Sequences:

>Translated_263_residues
MRERIPFRCIGVEEAASIVGQGRALLLDVRDANAYAAGHVEGARNLAYSALSDVIGTTPKAQPVVIYCYHGNASREYAQT
LSDFGFAAVFSVDGGYEAWRAFESVPAPTAPLDAALSSWLVDHGFPADGVNATAENGMTPLMKASQEGLADVVAALIAAG
ATLDARNGDGNTALWLACVGNHPHIIDALVDAGITIDSLNDTGASSLMYAASAGKAEALEKLLARGADPRLETQDGFCAL
DLAANLECLKLLRAATRREAAPA
>Mature_263_residues
MRERIPFRCIGVEEAASIVGQGRALLLDVRDANAYAAGHVEGARNLAYSALSDVIGTTPKAQPVVIYCYHGNASREYAQT
LSDFGFAAVFSVDGGYEAWRAFESVPAPTAPLDAALSSWLVDHGFPADGVNATAENGMTPLMKASQEGLADVVAALIAAG
ATLDARNGDGNTALWLACVGNHPHIIDALVDAGITIDSLNDTGASSLMYAASAGKAEALEKLLARGADPRLETQDGFCAL
DLAANLECLKLLRAATRREAAPA

Specific function: Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide [H]

COG id: COG0666

COG function: function code R; FOG: Ankyrin repeat

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI12746412, Length=117, Percent_Identity=35.042735042735, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI268607595, Length=188, Percent_Identity=31.9148936170213, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI62988328, Length=188, Percent_Identity=31.9148936170213, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI32967601, Length=114, Percent_Identity=34.2105263157895, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI116063534, Length=112, Percent_Identity=34.8214285714286, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI24233530, Length=126, Percent_Identity=34.1269841269841, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46519154, Length=241, Percent_Identity=31.9502074688797, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI46519151, Length=241, Percent_Identity=31.9502074688797, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI38683807, Length=90, Percent_Identity=40, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI37620163, Length=90, Percent_Identity=40, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI46519147, Length=90, Percent_Identity=40, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI38683816, Length=90, Percent_Identity=40, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI52426737, Length=114, Percent_Identity=30.7017543859649, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI188595682, Length=114, Percent_Identity=30.7017543859649, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI52426735, Length=114, Percent_Identity=30.7017543859649, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI70780355, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1789831, Length=104, Percent_Identity=37.5, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17542700, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25145251, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71981389, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71981411, Length=114, Percent_Identity=35.9649122807018, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71981414, Length=114, Percent_Identity=35.9649122807018, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24663102, Length=122, Percent_Identity=39.344262295082, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24663106, Length=122, Percent_Identity=39.344262295082, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24663095, Length=122, Percent_Identity=39.344262295082, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21357681, Length=122, Percent_Identity=39.344262295082, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24663091, Length=122, Percent_Identity=39.344262295082, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24650843, Length=144, Percent_Identity=32.6388888888889, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581260, Length=116, Percent_Identity=35.3448275862069, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581258, Length=116, Percent_Identity=35.3448275862069, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281359521, Length=116, Percent_Identity=33.6206896551724, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281359519, Length=116, Percent_Identity=33.6206896551724, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28558752, Length=116, Percent_Identity=33.6206896551724, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28558750, Length=116, Percent_Identity=33.6206896551724, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28558756, Length=116, Percent_Identity=33.6206896551724, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28558754, Length=116, Percent_Identity=33.6206896551724, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 27359; Mature: 27359

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50088 ANK_REPEAT ; PS50297 ANK_REP_REGION ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERIPFRCIGVEEAASIVGQGRALLLDVRDANAYAAGHVEGARNLAYSALSDVIGTTPK
CCCCCCEEEECHHHHHHHHCCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHCCCCC
AQPVVIYCYHGNASREYAQTLSDFGFAAVFSVDGGYEAWRAFESVPAPTAPLDAALSSWL
CCCEEEEEEECCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHH
VDHGFPADGVNATAENGMTPLMKASQEGLADVVAALIAAGATLDARNGDGNTALWLACVG
HHCCCCCCCCCCCHHCCCCHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCEEEEEEEEC
NHPHIIDALVDAGITIDSLNDTGASSLMYAASAGKAEALEKLLARGADPRLETQDGFCAL
CCCHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEE
DLAANLECLKLLRAATRREAAPA
EHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRERIPFRCIGVEEAASIVGQGRALLLDVRDANAYAAGHVEGARNLAYSALSDVIGTTPK
CCCCCCEEEECHHHHHHHHCCCCEEEEEEECCCCEECCCCHHHHHHHHHHHHHHHCCCCC
AQPVVIYCYHGNASREYAQTLSDFGFAAVFSVDGGYEAWRAFESVPAPTAPLDAALSSWL
CCCEEEEEEECCCHHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHH
VDHGFPADGVNATAENGMTPLMKASQEGLADVVAALIAAGATLDARNGDGNTALWLACVG
HHCCCCCCCCCCCHHCCCCHHHHHHHCCHHHHHHHHHHCCCEEECCCCCCCEEEEEEEEC
NHPHIIDALVDAGITIDSLNDTGASSLMYAASAGKAEALEKLLARGADPRLETQDGFCAL
CCCHHHHHHHHCCCEEECCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEE
DLAANLECLKLLRAATRREAAPA
EHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA