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The map label for this gene is nifA [H]

Identifier: 154244040

GI number: 154244040

Start: 88123

End: 89916

Strand: Direct

Name: nifA [H]

Synonym: Xaut_0082

Alternate gene names: 154244040

Gene position: 88123-89916 (Clockwise)

Preceding gene: 154244039

Following gene: 154244041

Centisome position: 1.66

GC content: 66.11

Gene sequence:

>1794_bases
ATGGTTCATAACGAAGCGTTGCAAGTCGAGCGCGAGCAGCCCCGGGCTCATGTTCCCCTCGTATCCCTGAGCGATGTGGC
GCTCACCGGGATCTACGAGATCTCGAAGATTTTGACGGCGCCGAACCGGCTTGAAACCACGCTGTCGAGCGTGGTCAGCC
TGCTTTCGTCCTTCATGCAGATGCGCCATGGCGTTATTTCGCTGCTGGAAGACGACGGAATTCCCAACATCACAGTGGGC
GCCGGCTGGAACGAGGGCACCGATGCGCGCTACCGCGCACGCCTTCCGGAGAAGGCGATCGGCCAGGTGATCGCCACCGC
CGTGCCGCTGATCGCGGAGAACGTTGCTTCCCATCCCGCTTTCGCCCCGGCCGATGTGGCCGCGCTGGGCGCCAGCGAGG
AGACCCGCGTCTCCTGGATCGGTGTTCCGCTGCGCGTCGGCGCGCGCGTTATCGGCACCCTCACCATCGACCGGGTGTGG
GACGGGCGCTCCGTGTTCCGCCTCGATTCCGACGTGCGCTTCCTGACCATGGTGGCGAACCTGATTGGCCAGACCGTGCA
GCTGCATCGCGTTGTCTCCCGCGATCGCGAGCGCCTGATGGCCGAGAGCGGGCGCCTGCAGAAGGAATTGTCCGAGCTGA
AGCCCCATACGGGCAGCCGCGAGCGCAAGCGCGTCTTTGTGGACGGCATCATCGGCGAGAGCCGGGAGATCCGCGCGCTG
CTCGACAAGATCATGATTGTCGCCAAGTCCCATTCGCCCGTGCTGCTGCGCGGCGAGAGCGGCACCGGCAAGGAGTTGAT
CGCCAAGGCGATCCACGAGATGTCGCCGCGGGCCAAGGGCCCCTTCATCAAGCTGAACTGCGCCGCGCTGCCGGAATCCG
TTCTCGAATCCGAACTGTTCGGTCACGAGAAGGGTGCCTTCACCGGCGCCGTGGGGGCCCGCAAGGGTCGCTTCGAACTG
GCGGACAAGGGCACGCTCTTCCTCGACGAGATCGGCGAGATCTCGCCCTCGTTCCAGGCCAAGCTGCTGCGCGTGCTGCA
GGAACAGGAGTTCGAGCGGGTCGGTGGTTCCCACACGCTGAAGGTGAACGTGCGTGTCGTCGCCGCCACCAACCGCAATC
TTGAGGAAGCGGTGGCGAAGAACGAGTTCCGCGCCGATCTCTACTACCGCATCAGCGTCATCCCGGTGATCGTGCCGTCG
CTGCGCGATCGCCGCGTGGATATCCCGCTGCTCGCCAACGAGTTCCTCGATCGCTTCAACCGTGAGAACGGTCGCGATCT
GGCGTTCAATCCCGACGCCATGGAAGTCCTTATGGGCTGCGGTTTCCCCGGCAATGTGCGTGAGCTTGAAAACTGCGTGC
AGCGCACCGCCACCCTTGCCCAGGGCGGCGCCATCGTCAGCGACGACTTCGCCTGCAAGCACAACGAGTGTCTGTCGGCG
CTGCTGTGGCGCAGCCCGGCCGAAGCGGCGACGCGCCGGCCCGTGGAAATCCCCTTGCCGGTGATGCCGTCGGTGGTCGC
TCCCATTGGCGGCGAGCCGGCCAATTCCAATACCGTGACCAAGCTCCCGCTGCCCTTGGTCGCGCCGCAGCCCCTCTTGC
CGGCCGCCGCTCCGGCGGTGGCGCCAGCCCGGCCGACCGCGCTGGACGAGGACTTGTCTGAGCGTGAGCGGCTGGTGGAC
GCCATGGAGCGGGCCGGCTGGGTGCAGGCGAAGGCCGCGCGGCTGCTGGGCCTGACCCCGCGCCAGATTGGCTACGCGCT
GAAGAAGCACGACATCGAGATCAAGCGCTTCTGA

Upstream 100 bases:

>100_bases
TGCCCTTACCTTGGGCACGACATGAGAATGAAACTAAATCAAAGTGTCGCTAATCATACAAAAAATTGGGATGGGAGCCT
GAGGTGACGGAGACAGCACC

Downstream 100 bases:

>100_bases
CGTCTTCCACTCTCGTCCGGATCCGGCCGAGCCTTCGGGTGTTCGGCCTTGAACCCGAAGTCGGCTGCGGCCGCCTTCGG
GTTCCGGCGTTATGGGGTCC

Product: transcriptional regulator NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MVHNEALQVEREQPRAHVPLVSLSDVALTGIYEISKILTAPNRLETTLSSVVSLLSSFMQMRHGVISLLEDDGIPNITVG
AGWNEGTDARYRARLPEKAIGQVIATAVPLIAENVASHPAFAPADVAALGASEETRVSWIGVPLRVGARVIGTLTIDRVW
DGRSVFRLDSDVRFLTMVANLIGQTVQLHRVVSRDRERLMAESGRLQKELSELKPHTGSRERKRVFVDGIIGESREIRAL
LDKIMIVAKSHSPVLLRGESGTGKELIAKAIHEMSPRAKGPFIKLNCAALPESVLESELFGHEKGAFTGAVGARKGRFEL
ADKGTLFLDEIGEISPSFQAKLLRVLQEQEFERVGGSHTLKVNVRVVAATNRNLEEAVAKNEFRADLYYRISVIPVIVPS
LRDRRVDIPLLANEFLDRFNRENGRDLAFNPDAMEVLMGCGFPGNVRELENCVQRTATLAQGGAIVSDDFACKHNECLSA
LLWRSPAEAATRRPVEIPLPVMPSVVAPIGGEPANSNTVTKLPLPLVAPQPLLPAAAPAVAPARPTALDEDLSERERLVD
AMERAGWVQAKAARLLGLTPRQIGYALKKHDIEIKRF

Sequences:

>Translated_597_residues
MVHNEALQVEREQPRAHVPLVSLSDVALTGIYEISKILTAPNRLETTLSSVVSLLSSFMQMRHGVISLLEDDGIPNITVG
AGWNEGTDARYRARLPEKAIGQVIATAVPLIAENVASHPAFAPADVAALGASEETRVSWIGVPLRVGARVIGTLTIDRVW
DGRSVFRLDSDVRFLTMVANLIGQTVQLHRVVSRDRERLMAESGRLQKELSELKPHTGSRERKRVFVDGIIGESREIRAL
LDKIMIVAKSHSPVLLRGESGTGKELIAKAIHEMSPRAKGPFIKLNCAALPESVLESELFGHEKGAFTGAVGARKGRFEL
ADKGTLFLDEIGEISPSFQAKLLRVLQEQEFERVGGSHTLKVNVRVVAATNRNLEEAVAKNEFRADLYYRISVIPVIVPS
LRDRRVDIPLLANEFLDRFNRENGRDLAFNPDAMEVLMGCGFPGNVRELENCVQRTATLAQGGAIVSDDFACKHNECLSA
LLWRSPAEAATRRPVEIPLPVMPSVVAPIGGEPANSNTVTKLPLPLVAPQPLLPAAAPAVAPARPTALDEDLSERERLVD
AMERAGWVQAKAARLLGLTPRQIGYALKKHDIEIKRF
>Mature_597_residues
MVHNEALQVEREQPRAHVPLVSLSDVALTGIYEISKILTAPNRLETTLSSVVSLLSSFMQMRHGVISLLEDDGIPNITVG
AGWNEGTDARYRARLPEKAIGQVIATAVPLIAENVASHPAFAPADVAALGASEETRVSWIGVPLRVGARVIGTLTIDRVW
DGRSVFRLDSDVRFLTMVANLIGQTVQLHRVVSRDRERLMAESGRLQKELSELKPHTGSRERKRVFVDGIIGESREIRAL
LDKIMIVAKSHSPVLLRGESGTGKELIAKAIHEMSPRAKGPFIKLNCAALPESVLESELFGHEKGAFTGAVGARKGRFEL
ADKGTLFLDEIGEISPSFQAKLLRVLQEQEFERVGGSHTLKVNVRVVAATNRNLEEAVAKNEFRADLYYRISVIPVIVPS
LRDRRVDIPLLANEFLDRFNRENGRDLAFNPDAMEVLMGCGFPGNVRELENCVQRTATLAQGGAIVSDDFACKHNECLSA
LLWRSPAEAATRRPVEIPLPVMPSVVAPIGGEPANSNTVTKLPLPLVAPQPLLPAAAPAVAPARPTALDEDLSERERLVD
AMERAGWVQAKAARLLGLTPRQIGYALKKHDIEIKRF

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=225, Percent_Identity=60, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=45.5384615384615, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1790437, Length=265, Percent_Identity=52.0754716981132, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI1789087, Length=328, Percent_Identity=43.2926829268293, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=55.793991416309, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1790299, Length=242, Percent_Identity=50.4132231404959, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788905, Length=260, Percent_Identity=46.9230769230769, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI87081872, Length=369, Percent_Identity=39.0243902439024, Blast_Score=215, Evalue=6e-57,
Organism=Escherichia coli, GI1789233, Length=242, Percent_Identity=44.6280991735537, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1786524, Length=258, Percent_Identity=43.0232558139535, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787583, Length=241, Percent_Identity=43.1535269709544, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789828, Length=251, Percent_Identity=37.4501992031873, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI87081858, Length=222, Percent_Identity=36.036036036036, Blast_Score=138, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 65304; Mature: 65304

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHNEALQVEREQPRAHVPLVSLSDVALTGIYEISKILTAPNRLETTLSSVVSLLSSFMQ
CCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MRHGVISLLEDDGIPNITVGAGWNEGTDARYRARLPEKAIGQVIATAVPLIAENVASHPA
HHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
FAPADVAALGASEETRVSWIGVPLRVGARVIGTLTIDRVWDGRSVFRLDSDVRFLTMVAN
CCCCHHHHCCCCCCCEEEEECCHHHHCCCEEEEEEEEEEECCCEEEEECCCHHHHHHHHH
LIGQTVQLHRVVSRDRERLMAESGRLQKELSELKPHTGSRERKRVFVDGIIGESREIRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEEEEECCCCCCHHHHHH
LDKIMIVAKSHSPVLLRGESGTGKELIAKAIHEMSPRAKGPFIKLNCAALPESVLESELF
HHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHH
GHEKGAFTGAVGARKGRFELADKGTLFLDEIGEISPSFQAKLLRVLQEQEFERVGGSHTL
CCCCCCEECCCCCCCCCEEECCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
KVNVRVVAATNRNLEEAVAKNEFRADLYYRISVIPVIVPSLRDRRVDIPLLANEFLDRFN
EEEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHC
RENGRDLAFNPDAMEVLMGCGFPGNVRELENCVQRTATLAQGGAIVSDDFACKHNECLSA
CCCCCEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHH
LLWRSPAEAATRRPVEIPLPVMPSVVAPIGGEPANSNTVTKLPLPLVAPQPLLPAAAPAV
HHHCCCHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
APARPTALDEDLSERERLVDAMERAGWVQAKAARLLGLTPRQIGYALKKHDIEIKRF
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MVHNEALQVEREQPRAHVPLVSLSDVALTGIYEISKILTAPNRLETTLSSVVSLLSSFMQ
CCCCHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
MRHGVISLLEDDGIPNITVGAGWNEGTDARYRARLPEKAIGQVIATAVPLIAENVASHPA
HHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
FAPADVAALGASEETRVSWIGVPLRVGARVIGTLTIDRVWDGRSVFRLDSDVRFLTMVAN
CCCCHHHHCCCCCCCEEEEECCHHHHCCCEEEEEEEEEEECCCEEEEECCCHHHHHHHHH
LIGQTVQLHRVVSRDRERLMAESGRLQKELSELKPHTGSRERKRVFVDGIIGESREIRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCEEEEECCCCCCHHHHHH
LDKIMIVAKSHSPVLLRGESGTGKELIAKAIHEMSPRAKGPFIKLNCAALPESVLESELF
HHHHHHEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHH
GHEKGAFTGAVGARKGRFELADKGTLFLDEIGEISPSFQAKLLRVLQEQEFERVGGSHTL
CCCCCCEECCCCCCCCCEEECCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
KVNVRVVAATNRNLEEAVAKNEFRADLYYRISVIPVIVPSLRDRRVDIPLLANEFLDRFN
EEEEEEEEECCCCHHHHHHHHHHHHHHEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHC
RENGRDLAFNPDAMEVLMGCGFPGNVRELENCVQRTATLAQGGAIVSDDFACKHNECLSA
CCCCCEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHH
LLWRSPAEAATRRPVEIPLPVMPSVVAPIGGEPANSNTVTKLPLPLVAPQPLLPAAAPAV
HHHCCCHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCC
APARPTALDEDLSERERLVDAMERAGWVQAKAARLLGLTPRQIGYALKKHDIEIKRF
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3313281; 12597275; 3357773; 2792368 [H]