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The map label for this gene is cysK [H]

Identifier: 154244033

GI number: 154244033

Start: 80313

End: 81299

Strand: Reverse

Name: cysK [H]

Synonym: Xaut_0075

Alternate gene names: 154244033

Gene position: 81299-80313 (Counterclockwise)

Preceding gene: 154244034

Following gene: 154244031

Centisome position: 1.53

GC content: 69.2

Gene sequence:

>987_bases
ATGGCAGGACCTGCCGCCAAGATCGTGCCCGCCGCCCAGCCTCGTCCGGGGCGCGGCCGCGTCTATGATTCCATCACCGA
GACCATAGGCAACACGCCGCTGGTGCGGCTGAAGCGCCTCGCCGAGCTGCGCGGGGCCAAAGCCGAGATTCTGGCCAAGC
TCGAATTCTTCAATCCCATCTCCAGCGTCAAGGACCGCATCGGCGTCGCCATGATCGAGGCGATGGAGGCGGCCGGCGTG
CTGGACCAGGACACGGTTCTGGTGGAGCCCACTTCCGGCAATACCGGCATCGCGCTGGCCTTCGTGGCAGCGGCCCGCGG
CTACCGGCTGATCCTGGTGATGCCCGAGAGCATGTCGGTGGAGCGCCGCAAGATGCTCGCCATTCTCGGCGCCGAGCTGG
TGCTGACCCCGGCGAACCTGGGCATGAAGGGCGCCGTCGCCCGCGCCGAGCAGCTCATCGCCGAGTTGCCCAAGGCGGCC
ATGCCCCAGCAGTTCAAGAACAAGGCCAACCCGGACATCCACCGCCGCACCACGGCCGAGGAGATTTGGAACGACACCGA
CGGCAAGGTGGACGCGGTGGTGGCCGGTGTCGGCACCGGCGGCACCATCACCGGCATCGGCCAGGTGCTGAAGCCGCGCA
AGGCCTCGCTCAAGATGATCGCGGTGGAGCCCGAGGATTCCCCGGTGCTGTCCGGCGGCTCCCCCGGCCCCCACAAGATC
CAGGGCATCGGCGCCGGCTTCGTGCCGGACGTGCTCGACCGCTCCATCATCGACGAGGTGGTGACGGTGGGCAACCAGAC
CGCGTTCGACACCGCGCGGGCGCTGGCCCGCTACGAGGGCATCCCCGGCGGGATCTCCTCCGGCGCGGCCGTGGCGGCGG
CGCTGGAGATCGCGGTGCGCGACGACATGGCCGGCAAGTCCATCGTGGTGATCGTGCCGTCCTTCGCCGAGCGCTATCTC
TCGACGGCCCTGTTCGAAGGCCTCTGA

Upstream 100 bases:

>100_bases
GATGGCAAGGGCCACGGTTCGGAGCACGAGAGGATCGATTTCGCCATTTGAGAGGTACGCCCCCCGCGTGCCGGCGACCA
ATTTCCCAAGGAGATCGCCC

Downstream 100 bases:

>100_bases
GAGCGCCCTTGCGGCTGAAAATAGTCTCGCGTTCCTGCGCCCCGGCCCCGGCCGGGGCGCAACTGTTATAAGGCCTTGTC
CTCCCACGCCCCGTCGCGGA

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAGPAAKIVPAAQPRPGRGRVYDSITETIGNTPLVRLKRLAELRGAKAEILAKLEFFNPISSVKDRIGVAMIEAMEAAGV
LDQDTVLVEPTSGNTGIALAFVAAARGYRLILVMPESMSVERRKMLAILGAELVLTPANLGMKGAVARAEQLIAELPKAA
MPQQFKNKANPDIHRRTTAEEIWNDTDGKVDAVVAGVGTGGTITGIGQVLKPRKASLKMIAVEPEDSPVLSGGSPGPHKI
QGIGAGFVPDVLDRSIIDEVVTVGNQTAFDTARALARYEGIPGGISSGAAVAAALEIAVRDDMAGKSIVVIVPSFAERYL
STALFEGL

Sequences:

>Translated_328_residues
MAGPAAKIVPAAQPRPGRGRVYDSITETIGNTPLVRLKRLAELRGAKAEILAKLEFFNPISSVKDRIGVAMIEAMEAAGV
LDQDTVLVEPTSGNTGIALAFVAAARGYRLILVMPESMSVERRKMLAILGAELVLTPANLGMKGAVARAEQLIAELPKAA
MPQQFKNKANPDIHRRTTAEEIWNDTDGKVDAVVAGVGTGGTITGIGQVLKPRKASLKMIAVEPEDSPVLSGGSPGPHKI
QGIGAGFVPDVLDRSIIDEVVTVGNQTAFDTARALARYEGIPGGISSGAAVAAALEIAVRDDMAGKSIVVIVPSFAERYL
STALFEGL
>Mature_327_residues
AGPAAKIVPAAQPRPGRGRVYDSITETIGNTPLVRLKRLAELRGAKAEILAKLEFFNPISSVKDRIGVAMIEAMEAAGVL
DQDTVLVEPTSGNTGIALAFVAAARGYRLILVMPESMSVERRKMLAILGAELVLTPANLGMKGAVARAEQLIAELPKAAM
PQQFKNKANPDIHRRTTAEEIWNDTDGKVDAVVAGVGTGGTITGIGQVLKPRKASLKMIAVEPEDSPVLSGGSPGPHKIQ
GIGAGFVPDVLDRSIIDEVVTVGNQTAFDTARALARYEGIPGGISSGAAVAAALEIAVRDDMAGKSIVVIVPSFAERYLS
TALFEGL

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=329, Percent_Identity=41.6413373860182, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI295821200, Length=329, Percent_Identity=41.6413373860182, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4557415, Length=329, Percent_Identity=41.6413373860182, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1788754, Length=318, Percent_Identity=55.6603773584906, Blast_Score=300, Evalue=8e-83,
Organism=Escherichia coli, GI2367138, Length=302, Percent_Identity=39.7350993377483, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=50.4950495049505, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=51, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=51.5254237288136, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI115535073, Length=309, Percent_Identity=51.7799352750809, Blast_Score=273, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI17561720, Length=309, Percent_Identity=50.8090614886731, Blast_Score=257, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=50.4716981132076, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17534315, Length=327, Percent_Identity=38.8379204892966, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=321, Percent_Identity=37.3831775700935, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=50.4132231404959, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321594, Length=318, Percent_Identity=37.7358490566038, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=348, Percent_Identity=31.6091954022989, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24643623, Length=312, Percent_Identity=39.1025641025641, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI20129101, Length=312, Percent_Identity=39.1025641025641, Blast_Score=197, Evalue=9e-51,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34307; Mature: 34176

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGPAAKIVPAAQPRPGRGRVYDSITETIGNTPLVRLKRLAELRGAKAEILAKLEFFNPI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHH
SSVKDRIGVAMIEAMEAAGVLDQDTVLVEPTSGNTGIALAFVAAARGYRLILVMPESMSV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCH
ERRKMLAILGAELVLTPANLGMKGAVARAEQLIAELPKAAMPQQFKNKANPDIHRRTTAE
HHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHCCCCCHHHHHHHH
EIWNDTDGKVDAVVAGVGTGGTITGIGQVLKPRKASLKMIAVEPEDSPVLSGGSPGPHKI
HHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEE
QGIGAGFVPDVLDRSIIDEVVTVGNQTAFDTARALARYEGIPGGISSGAAVAAALEIAVR
EECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DDMAGKSIVVIVPSFAERYLSTALFEGL
CCCCCCEEEEEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGPAAKIVPAAQPRPGRGRVYDSITETIGNTPLVRLKRLAELRGAKAEILAKLEFFNPI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHH
SSVKDRIGVAMIEAMEAAGVLDQDTVLVEPTSGNTGIALAFVAAARGYRLILVMPESMSV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCH
ERRKMLAILGAELVLTPANLGMKGAVARAEQLIAELPKAAMPQQFKNKANPDIHRRTTAE
HHHHHHHHHCCHHEEECCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHCCCCCHHHHHHHH
EIWNDTDGKVDAVVAGVGTGGTITGIGQVLKPRKASLKMIAVEPEDSPVLSGGSPGPHKI
HHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCEE
QGIGAGFVPDVLDRSIIDEVVTVGNQTAFDTARALARYEGIPGGISSGAAVAAALEIAVR
EECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
DDMAGKSIVVIVPSFAERYLSTALFEGL
CCCCCCEEEEEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]