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The map label for this gene is mcbR [H]

Identifier: 154243968

GI number: 154243968

Start: 10609

End: 11328

Strand: Reverse

Name: mcbR [H]

Synonym: Xaut_0010

Alternate gene names: 154243968

Gene position: 11328-10609 (Counterclockwise)

Preceding gene: 154243969

Following gene: NA

Centisome position: 0.21

GC content: 68.33

Gene sequence:

>720_bases
GTGGATGCCATGGCCGAGTTGCGGCAGATCGAGAAGGAAAATCTATCGGCCCGCGTCTACGGCCGCATCCGCCGGGCGCT
GATCGACGGGCAGTTCGAGCCCGGCGAGCGGCTGCGCATCTCCGCGCTCGCGGCCGATCTGGGCACCTCCATCACCCCGG
TGCGCGAGGCCATCTTCCGCCTGGTGAGCGAGCAGGCGCTGGAGATGAAGGCCGCCACCGCCATTCATGTGCCGCTCTTG
GACAGCGCCCGGCTGAAGGAGATCCAGCTCATCCGCATGGAGCTGGAGGGCGCCGCCTCCGCCCGCGCCGCCCAGCGCAT
CACCGACAAGCAGATCCGCCGGCTGGAGCAGATCCAGGACGAGTTCATCGAGGCCGCCGCCACCAACCCGAAGAAGGCGA
GCCTGAAGAACCGCGACTTCCACTTCGCGCTGATCGAGATCGCCGAGCTGCCGCTGCTCGAATCCATCGTGGAGAATATG
TGGACGCTGATGGGACCGCTGCTCAGCGTGTTCCACTCCACCTTGCCGCCGCGCCAGATCGCCGGCGAGGACCACCGCCA
CTATGACGTGCTGGAAGCGCTCCGGGCGCGCGATCCGGAGCGGGCGCAGCATGCCATCCAGCAGGACATCGCCTGGGGCC
TCGTCATGGCCGACTGGCTGGACGAGCAGGCCAAGGCCCCGCCCGGCAAGCGCAAGCTCGCCTCCCTCACCGCCGAGTGA

Upstream 100 bases:

>100_bases
GGACGCGCTTGTGGAGGATGCAATGCAGGGGAGCAAATCCCCCAGCACCGCACCCGGGCTGGCCGTGTGATATAGGGTGT
ATCCGGGTCCTTTCAGAAGC

Downstream 100 bases:

>100_bases
GGACGCAAACCGGAACCACCGTCATCCGTCTCGCACCCACCGTCATCCTCCGGCTCGACCGGAGGATCCATAGGGCGGCC
GGGACGCGCTGCCGCCTTCA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MDAMAELRQIEKENLSARVYGRIRRALIDGQFEPGERLRISALAADLGTSITPVREAIFRLVSEQALEMKAATAIHVPLL
DSARLKEIQLIRMELEGAASARAAQRITDKQIRRLEQIQDEFIEAAATNPKKASLKNRDFHFALIEIAELPLLESIVENM
WTLMGPLLSVFHSTLPPRQIAGEDHRHYDVLEALRARDPERAQHAIQQDIAWGLVMADWLDEQAKAPPGKRKLASLTAE

Sequences:

>Translated_239_residues
MDAMAELRQIEKENLSARVYGRIRRALIDGQFEPGERLRISALAADLGTSITPVREAIFRLVSEQALEMKAATAIHVPLL
DSARLKEIQLIRMELEGAASARAAQRITDKQIRRLEQIQDEFIEAAATNPKKASLKNRDFHFALIEIAELPLLESIVENM
WTLMGPLLSVFHSTLPPRQIAGEDHRHYDVLEALRARDPERAQHAIQQDIAWGLVMADWLDEQAKAPPGKRKLASLTAE
>Mature_239_residues
MDAMAELRQIEKENLSARVYGRIRRALIDGQFEPGERLRISALAADLGTSITPVREAIFRLVSEQALEMKAATAIHVPLL
DSARLKEIQLIRMELEGAASARAAQRITDKQIRRLEQIQDEFIEAAATNPKKASLKNRDFHFALIEIAELPLLESIVENM
WTLMGPLLSVFHSTLPPRQIAGEDHRHYDVLEALRARDPERAQHAIQQDIAWGLVMADWLDEQAKAPPGKRKLASLTAE

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=203, Percent_Identity=33.4975369458128, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 26894; Mature: 26894

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAMAELRQIEKENLSARVYGRIRRALIDGQFEPGERLRISALAADLGTSITPVREAIFR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
LVSEQALEMKAATAIHVPLLDSARLKEIQLIRMELEGAASARAAQRITDKQIRRLEQIQD
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFIEAAATNPKKASLKNRDFHFALIEIAELPLLESIVENMWTLMGPLLSVFHSTLPPRQI
HHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AGEDHRHYDVLEALRARDPERAQHAIQQDIAWGLVMADWLDEQAKAPPGKRKLASLTAE
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
>Mature Secondary Structure
MDAMAELRQIEKENLSARVYGRIRRALIDGQFEPGERLRISALAADLGTSITPVREAIFR
CCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
LVSEQALEMKAATAIHVPLLDSARLKEIQLIRMELEGAASARAAQRITDKQIRRLEQIQD
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFIEAAATNPKKASLKNRDFHFALIEIAELPLLESIVENMWTLMGPLLSVFHSTLPPRQI
HHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AGEDHRHYDVLEALRARDPERAQHAIQQDIAWGLVMADWLDEQAKAPPGKRKLASLTAE
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]