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The map label for this gene is ribE [H]

Identifier: 153950122

GI number: 153950122

Start: 2027545

End: 2028201

Strand: Direct

Name: ribE [H]

Synonym: YpsIP31758_1751

Alternate gene names: 153950122

Gene position: 2027545-2028201 (Clockwise)

Preceding gene: 153948284

Following gene: 153950182

Centisome position: 42.93

GC content: 45.81

Gene sequence:

>657_bases
ATGTTTACCGGTATTGTTCAAGGCACCGGGCCTGTGGTGGCCATCGAAGAAAAATCTAATTTCCGCACGCATGTTGTTGA
GCTGCCTATGGATATGCTGCCTGAGTTGGCATTGGGGGCGTCAGTTGCTCACAATGGATGCTGCCTCACCGTGACACACA
TTGAGGGCAATCGTGTCAGTTTTGATTTGATGAAGGAAACATTACGCCTCACTAACTTAGGGGATATAAACGTTGGTGAT
AAGGTTAATCTAGAGAGGGCGGCTAAATTCAGCGATGAAATTGGTGGTCATCTTATGTCCGGTCATATTATCTGTACTGC
AGAGATTGCTAAAATATACACATCAGAAAATAACCGTCAGATTTGGTTTCGTATGCCCAGCGAAGATCTAATGAAATATG
TGTTACATAAAGGTTTTATTGGTATTGATGGCATCAGCCTGACAATCGGCGAGGTGGTGGGTAATCGTTTCTGTGTTCAT
CTCATTCCAGAAACGTTGTCTCGTACCACACTGGGTAAAAAGCGGTTAGGGCACCGGGTTAATATCGAGATAGATCCGCA
GACCCAAGCGGTGGTTGATACTGTTGAACGGGTATTGGCGCAGCGCAATATTGCTAACGCGGCGTTAGTGGACGAAAAAG
TCGTGCGGGTGTCATAA

Upstream 100 bases:

>100_bases
GTCTTTTTTGCTCTAAGTGGTTTGGTTTTCCTGATTTTCTGAGGCAAAATTGACACAGCTAATATTTCTTTCTACAGATA
ACTTATAAGAGGCATGGCGT

Downstream 100 bases:

>100_bases
CCTACAAAAAATAAGACAGGGGTTTTTATCCCCCTGTCTCCTATCTGAGGTTAGCGAGGGACGCGAATACCCCCATTCAC
ACCATTTGGACTGAATAGTA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MFTGIVQGTGPVVAIEEKSNFRTHVVELPMDMLPELALGASVAHNGCCLTVTHIEGNRVSFDLMKETLRLTNLGDINVGD
KVNLERAAKFSDEIGGHLMSGHIICTAEIAKIYTSENNRQIWFRMPSEDLMKYVLHKGFIGIDGISLTIGEVVGNRFCVH
LIPETLSRTTLGKKRLGHRVNIEIDPQTQAVVDTVERVLAQRNIANAALVDEKVVRVS

Sequences:

>Translated_218_residues
MFTGIVQGTGPVVAIEEKSNFRTHVVELPMDMLPELALGASVAHNGCCLTVTHIEGNRVSFDLMKETLRLTNLGDINVGD
KVNLERAAKFSDEIGGHLMSGHIICTAEIAKIYTSENNRQIWFRMPSEDLMKYVLHKGFIGIDGISLTIGEVVGNRFCVH
LIPETLSRTTLGKKRLGHRVNIEIDPQTQAVVDTVERVLAQRNIANAALVDEKVVRVS
>Mature_218_residues
MFTGIVQGTGPVVAIEEKSNFRTHVVELPMDMLPELALGASVAHNGCCLTVTHIEGNRVSFDLMKETLRLTNLGDINVGD
KVNLERAAKFSDEIGGHLMSGHIICTAEIAKIYTSENNRQIWFRMPSEDLMKYVLHKGFIGIDGISLTIGEVVGNRFCVH
LIPETLSRTTLGKKRLGHRVNIEIDPQTQAVVDTVERVLAQRNIANAALVDEKVVRVS

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=205, Percent_Identity=79.5121951219512, Blast_Score=334, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319733, Length=195, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 24029; Mature: 24029

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQGTGPVVAIEEKSNFRTHVVELPMDMLPELALGASVAHNGCCLTVTHIEGNRVS
CCCEEEECCCCEEEEECCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEEEEECCCEEE
FDLMKETLRLTNLGDINVGDKVNLERAAKFSDEIGGHLMSGHIICTAEIAKIYTSENNRQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCEEEEEEEEEEEEECCCCCE
IWFRMPSEDLMKYVLHKGFIGIDGISLTIGEVVGNRFCVHLIPETLSRTTLGKKRLGHRV
EEEECCHHHHHHHHHHCCCEECCCCEEEHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEE
NIEIDPQTQAVVDTVERVLAQRNIANAALVDEKVVRVS
EEEECCCHHHHHHHHHHHHHHCCCCHHHHHCCEEEECC
>Mature Secondary Structure
MFTGIVQGTGPVVAIEEKSNFRTHVVELPMDMLPELALGASVAHNGCCLTVTHIEGNRVS
CCCEEEECCCCEEEEECCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEEEEECCCEEE
FDLMKETLRLTNLGDINVGDKVNLERAAKFSDEIGGHLMSGHIICTAEIAKIYTSENNRQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCEEECCEEEEEEEEEEEEECCCCCE
IWFRMPSEDLMKYVLHKGFIGIDGISLTIGEVVGNRFCVHLIPETLSRTTLGKKRLGHRV
EEEECCHHHHHHHHHHCCCEECCCCEEEHHHHHCCEEEEEECHHHHHHHHHHHHHCCCEE
NIEIDPQTQAVVDTVERVLAQRNIANAALVDEKVVRVS
EEEECCCHHHHHHHHHHHHHHCCCCHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]