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The map label for this gene is nudE [H]

Identifier: 153949897

GI number: 153949897

Start: 4456276

End: 4456821

Strand: Reverse

Name: nudE [H]

Synonym: YpsIP31758_3973

Alternate gene names: 153949897

Gene position: 4456821-4456276 (Counterclockwise)

Preceding gene: 153948119

Following gene: 153947846

Centisome position: 94.36

GC content: 47.44

Gene sequence:

>546_bases
ATGAAACACCTGCAAAAACCTAAAATTCTGAAAATAGAAACGGTTGCCTGCTCCCGCTTATTCAATGTTGAAGCGGTAGA
ACTGGAGTTCAGTAATGGCGTACAGCGCATTTATGAACGTATGCGGCCATCGAATCGTGAGGCCGTGATGATTGTGCCGG
TTATCGGTAACGATTTACTGCTCATTCGTGAATACGCTGTCGGTATTGAGGAATACGAATTGGGCTTTCCTAAAGGTCTG
ATAGATCCCGGTGAAGGGGTGCTGGAAGCTGCGAACCGCGAGTTAATGGAAGAAGTTGGATATGGCGCTGAGCGCTTTGA
CTTTCTCGCTAAACTGACCATGGCACCGTCCTACTTTTCCAGCAAAATGAATATTGTTATTGCCCACGGCCTGTATCCAC
AAAGTTTGGAAGGTGATGAGCCTGAGCCATTGCCGCAAGTGCGGTGGCCAATTGCTAATATGATGGCACTCCTTAATGAA
GCTGATTTCCGCGAGGCCCGTAATGTCAGCGCCCTTTTTTTGGCACATACCTTTCTGAATCAGTGA

Upstream 100 bases:

>100_bases
AAATCACACAAAGTATCTGGATAATATTAACGGATAATATCGCGTTTTCGTTAATTCAGATATGTGTTAAATGGCGGTGA
GTGATTAATTTGGGGCTATC

Downstream 100 bases:

>100_bases
TTATTTTGATGAGTCACTACCGTGAGATGAATTTATTTCTGACGTAACTTATTTTTTATACGAGTAATTTATAAAAAAAC
CAGATGATCATCAGATCATC

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKHLQKPKILKIETVACSRLFNVEAVELEFSNGVQRIYERMRPSNREAVMIVPVIGNDLLLIREYAVGIEEYELGFPKGL
IDPGEGVLEAANRELMEEVGYGAERFDFLAKLTMAPSYFSSKMNIVIAHGLYPQSLEGDEPEPLPQVRWPIANMMALLNE
ADFREARNVSALFLAHTFLNQ

Sequences:

>Translated_181_residues
MKHLQKPKILKIETVACSRLFNVEAVELEFSNGVQRIYERMRPSNREAVMIVPVIGNDLLLIREYAVGIEEYELGFPKGL
IDPGEGVLEAANRELMEEVGYGAERFDFLAKLTMAPSYFSSKMNIVIAHGLYPQSLEGDEPEPLPQVRWPIANMMALLNE
ADFREARNVSALFLAHTFLNQ
>Mature_181_residues
MKHLQKPKILKIETVACSRLFNVEAVELEFSNGVQRIYERMRPSNREAVMIVPVIGNDLLLIREYAVGIEEYELGFPKGL
IDPGEGVLEAANRELMEEVGYGAERFDFLAKLTMAPSYFSSKMNIVIAHGLYPQSLEGDEPEPLPQVRWPIANMMALLNE
ADFREARNVSALFLAHTFLNQ

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=178, Percent_Identity=74.7191011235955, Blast_Score=276, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 20499; Mature: 20499

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLQKPKILKIETVACSRLFNVEAVELEFSNGVQRIYERMRPSNREAVMIVPVIGNDLL
CCCCCCCCEEEEEHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
LIREYAVGIEEYELGFPKGLIDPGEGVLEAANRELMEEVGYGAERFDFLAKLTMAPSYFS
EEHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHC
SKMNIVIAHGLYPQSLEGDEPEPLPQVRWPIANMMALLNEADFREARNVSALFLAHTFLN
CCCEEEEEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
Q
C
>Mature Secondary Structure
MKHLQKPKILKIETVACSRLFNVEAVELEFSNGVQRIYERMRPSNREAVMIVPVIGNDLL
CCCCCCCCEEEEEHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEEECCCCEE
LIREYAVGIEEYELGFPKGLIDPGEGVLEAANRELMEEVGYGAERFDFLAKLTMAPSYFS
EEHHHHCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHC
SKMNIVIAHGLYPQSLEGDEPEPLPQVRWPIANMMALLNEADFREARNVSALFLAHTFLN
CCCEEEEEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]