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The map label for this gene is btuE [H]

Identifier: 153949548

GI number: 153949548

Start: 1990420

End: 1990974

Strand: Direct

Name: btuE [H]

Synonym: YpsIP31758_1721

Alternate gene names: 153949548

Gene position: 1990420-1990974 (Clockwise)

Preceding gene: 153948990

Following gene: 153950434

Centisome position: 42.14

GC content: 44.5

Gene sequence:

>555_bases
ATGAGTCACCCAATTTACGCCATTTCAGTGCAAACGATTGATCACCAGCTGGTTAAGTTGGCGAAGTATAAAGGTTCCGT
TTTATTAGTGGTGAATGTGGCTTCGCAATGTGGTTTGACTCAGCAATATGAAGGATTAGAAAGCCTCTATAAAACCTATC
AGAAGCAAGGTTTTGAGGTTTTAGGTTTCCCGAGTAATGAGTTTGCAGGACAAGAGCCTGGCAGTGATGAGGAAATTCAT
GCTTTCTGCCGTGGTACTTTTGGTGTCGACTTCCCCATGTTCAGTAAAATTGAAGTCAATGGTCCACATCGGCATCCTCT
ATATCAGCACTTGGTTACTGCTAAGCCTGTTGCAGTGAAACCGGAAGGAAGTGAATTTTATCAACGCCTGGCGAGTAAAG
GCCGTGAGCCAAAACAGCCGGGTGATATCCTATGGAATTTTGAGAAATTCTTGATTAGCCGTGATGGCACTGTCTTGGCG
CGTTTTGCTCCAGATATGGCACCAGAAGCACCTATTTTAATCAATGCCATCAATCAGGCACTGGCTAGCCACTAA

Upstream 100 bases:

>100_bases
GTTACTGATACGGGTGAAAGGAGTAAAGTGATTTGTCTTCTTATAAATGCCGCGGTGATTTTACCGGTTTATTATCATTA
CTGAATAAGGATCCAACCTG

Downstream 100 bases:

>100_bases
ATTACGGGTAACGAGTTTATTTTGATGCTATTGCAACTCAACAATGTCAGCGTAGGAACCCGCCTTGCTTCTTTTTCTTC
GCAGGTGACCACGGGGTTGC

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSHPIYAISVQTIDHQLVKLAKYKGSVLLVVNVASQCGLTQQYEGLESLYKTYQKQGFEVLGFPSNEFAGQEPGSDEEIH
AFCRGTFGVDFPMFSKIEVNGPHRHPLYQHLVTAKPVAVKPEGSEFYQRLASKGREPKQPGDILWNFEKFLISRDGTVLA
RFAPDMAPEAPILINAINQALASH

Sequences:

>Translated_184_residues
MSHPIYAISVQTIDHQLVKLAKYKGSVLLVVNVASQCGLTQQYEGLESLYKTYQKQGFEVLGFPSNEFAGQEPGSDEEIH
AFCRGTFGVDFPMFSKIEVNGPHRHPLYQHLVTAKPVAVKPEGSEFYQRLASKGREPKQPGDILWNFEKFLISRDGTVLA
RFAPDMAPEAPILINAINQALASH
>Mature_183_residues
SHPIYAISVQTIDHQLVKLAKYKGSVLLVVNVASQCGLTQQYEGLESLYKTYQKQGFEVLGFPSNEFAGQEPGSDEEIHA
FCRGTFGVDFPMFSKIEVNGPHRHPLYQHLVTAKPVAVKPEGSEFYQRLASKGREPKQPGDILWNFEKFLISRDGTVLAR
FAPDMAPEAPILINAINQALASH

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=165, Percent_Identity=40, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI75709200, Length=174, Percent_Identity=39.0804597701149, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI90903240, Length=174, Percent_Identity=39.0804597701149, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI192455698, Length=161, Percent_Identity=36.6459627329193, Blast_Score=101, Evalue=3e-22,
Organism=Homo sapiens, GI90903238, Length=147, Percent_Identity=40.1360544217687, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI4557629, Length=154, Percent_Identity=37.012987012987, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI6006001, Length=168, Percent_Identity=36.9047619047619, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI41406084, Length=166, Percent_Identity=35.5421686746988, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI33186887, Length=159, Percent_Identity=33.9622641509434, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI32967607, Length=171, Percent_Identity=30.4093567251462, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1788003, Length=182, Percent_Identity=59.8901098901099, Blast_Score=233, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI17506887, Length=166, Percent_Identity=39.7590361445783, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17535473, Length=166, Percent_Identity=39.1566265060241, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI193209493, Length=185, Percent_Identity=34.5945945945946, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17550320, Length=185, Percent_Identity=34.0540540540541, Blast_Score=112, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71993584, Length=165, Percent_Identity=34.5454545454545, Blast_Score=106, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI193210707, Length=171, Percent_Identity=35.672514619883, Blast_Score=99, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI71993573, Length=94, Percent_Identity=36.1702127659575, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71999642, Length=78, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319721, Length=170, Percent_Identity=40, Blast_Score=136, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322228, Length=147, Percent_Identity=43.5374149659864, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322826, Length=158, Percent_Identity=36.7088607594937, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24656772, Length=166, Percent_Identity=36.144578313253, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24656777, Length=166, Percent_Identity=36.144578313253, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24656767, Length=166, Percent_Identity=36.144578313253, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI85726443, Length=168, Percent_Identity=35.1190476190476, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20474; Mature: 20342

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPIYAISVQTIDHQLVKLAKYKGSVLLVVNVASQCGLTQQYEGLESLYKTYQKQGFEV
CCCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCHHHHHHHHHHHHHHHCCCEE
LGFPSNEFAGQEPGSDEEIHAFCRGTFGVDFPMFSKIEVNGPHRHPLYQHLVTAKPVAVK
EECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEC
PEGSEFYQRLASKGREPKQPGDILWNFEKFLISRDGTVLARFAPDMAPEAPILINAINQA
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHH
LASH
HHCC
>Mature Secondary Structure 
SHPIYAISVQTIDHQLVKLAKYKGSVLLVVNVASQCGLTQQYEGLESLYKTYQKQGFEV
CCCEEEEEHHHHHHHHHHHHHHCCCEEEEEEECHHCCCCHHHHHHHHHHHHHHHCCCEE
LGFPSNEFAGQEPGSDEEIHAFCRGTFGVDFPMFSKIEVNGPHRHPLYQHLVTAKPVAVK
EECCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEC
PEGSEFYQRLASKGREPKQPGDILWNFEKFLISRDGTVLARFAPDMAPEAPILINAINQA
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHH
LASH
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]