Click here to switch to the map view.

The map label for this gene is papD [H]

Identifier: 153949184

GI number: 153949184

Start: 727206

End: 727925

Strand: Direct

Name: papD [H]

Synonym: YpsIP31758_0615

Alternate gene names: 153949184

Gene position: 727206-727925 (Clockwise)

Preceding gene: 153948600

Following gene: 153949699

Centisome position: 15.4

GC content: 48.61

Gene sequence:

>720_bases
ATGAAGAAGCTTTCATTAATCGCATTGACCAGCACATTGGTATTCACCAGTTATGCCGCCAATGCCGCGATTGCATTAGA
TCGGACGCGAATTATTTATAACGGGCAGGATAAGTCCATCAGCCTGAATATCAGCAATGAAAATAAGACACTGCCTTATT
TAGCACAGGCATGGATTGAGAATGAACAACAGCAAAAAGTCACCACCCCGTTAGTGGCAACGCCGCCGGTTCAACGGGTG
GAAGCGGGTAAGAAAAGCGCGGTACGTATCTCTGCCACCCCTGAGGCGCTGAAATTGCCACAAGATCGCGAGTCACTGTT
CTACTTCAATCTGCGTGAAATTCCGCCTCGCAGTGAAAAATCCAATGTCATGCAGATTGCCTTACAAACCAAAATCAAAC
TGTTCTACCGACCAGAAGGGATTACCCCTGCCCCGGGCCAAATTTGGCAGGAACAACTGGTTCTGCATAAAACGGCGCAA
GGTTTTCGGCTAGAGAACCCCACTCCCTATTACATTACGGTCGTTGGGCTGGCGGAGAAAGGTAAGTCATCGGCTGACAG
CAATTTTCAACCTTTGATGGTTGCGCCCAAATCCAGTGCTGAGCTGAAATCTGCCAGTTTCACCGCCCCCGTCCTGACCT
ACATTAATGATTATGGTGGTCGACCAGAACTGAGTTTCAGTTGCAGTGGCAGCATTTGCCGGGCAGCTAAAACGATTTAA

Upstream 100 bases:

>100_bases
GCGCTAAGCAGTGCCAGGTGGCGATCCCAGCAGCATTACAGCCGCAGGCGCAAATATTATTACCCTGCATATCCGTTAAC
TAAGATTGAGGTGATATACC

Downstream 100 bases:

>100_bases
ACCGGTTGAAAGAGAGAACCATCAATGAAAAAAAATATATTCCTCATTTTACAGTGTGAGAGGTATAAGGCGGGACTGCT
GTTATTGCTACTCCTGTTAT

Product: chaperone protein PapD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKKLSLIALTSTLVFTSYAANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIENEQQQKVTTPLVATPPVQRV
EAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEKSNVMQIALQTKIKLFYRPEGITPAPGQIWQEQLVLHKTAQ
GFRLENPTPYYITVVGLAEKGKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSICRAAKTI

Sequences:

>Translated_239_residues
MKKLSLIALTSTLVFTSYAANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIENEQQQKVTTPLVATPPVQRV
EAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEKSNVMQIALQTKIKLFYRPEGITPAPGQIWQEQLVLHKTAQ
GFRLENPTPYYITVVGLAEKGKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSICRAAKTI
>Mature_239_residues
MKKLSLIALTSTLVFTSYAANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIENEQQQKVTTPLVATPPVQRV
EAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEKSNVMQIALQTKIKLFYRPEGITPAPGQIWQEQLVLHKTAQ
GFRLENPTPYYITVVGLAEKGKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSICRAAKTI

Specific function: Binds and caps interactive surfaces on pilus subunits to prevent them from participating in non-productive interactions. Facilitates the import of subunits into the periplasm. May facilitate subunit folding. Chaperone-subunit complexes are then targeted t

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1788677, Length=210, Percent_Identity=45.2380952380952, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1786936, Length=241, Percent_Identity=40.6639004149378, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI87082203, Length=235, Percent_Identity=36.5957446808511, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786333, Length=243, Percent_Identity=31.6872427983539, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1789532, Length=208, Percent_Identity=33.6538461538462, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788428, Length=229, Percent_Identity=31.0043668122271, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1790771, Length=228, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787171, Length=233, Percent_Identity=29.6137339055794, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786743, Length=227, Percent_Identity=30.3964757709251, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI87081806, Length=233, Percent_Identity=28.3261802575107, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26381; Mature: 26381

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSLIALTSTLVFTSYAANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIE
CCCEEEEEHHHHHHHHHHCCCEEEEEECEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
NEQQQKVTTPLVATPPVQRVEAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEK
CCCHHHHCCCEEECCCHHHHHCCCCCEEEEECCCHHHCCCCCCCEEEEEEEECCCCCCCC
SNVMQIALQTKIKLFYRPEGITPAPGQIWQEQLVLHKTAQGFRLENPTPYYITVVGLAEK
CCEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEECCC
GKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSICRAAKTI
CCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHCCC
>Mature Secondary Structure
MKKLSLIALTSTLVFTSYAANAAIALDRTRIIYNGQDKSISLNISNENKTLPYLAQAWIE
CCCEEEEEHHHHHHHHHHCCCEEEEEECEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
NEQQQKVTTPLVATPPVQRVEAGKKSAVRISATPEALKLPQDRESLFYFNLREIPPRSEK
CCCHHHHCCCEEECCCHHHHHCCCCCEEEEECCCHHHCCCCCCCEEEEEEEECCCCCCCC
SNVMQIALQTKIKLFYRPEGITPAPGQIWQEQLVLHKTAQGFRLENPTPYYITVVGLAEK
CCEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEECCC
GKSSADSNFQPLMVAPKSSAELKSASFTAPVLTYINDYGGRPELSFSCSGSICRAAKTI
CCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1357526; 10049807; 2478891; 10446050 [H]