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The map label for this gene is dnaB2 [H]

Identifier: 153948863

GI number: 153948863

Start: 4219538

End: 4220899

Strand: Reverse

Name: dnaB2 [H]

Synonym: YpsIP31758_3747

Alternate gene names: 153948863

Gene position: 4220899-4219538 (Counterclockwise)

Preceding gene: 153948317

Following gene: 153948935

Centisome position: 89.36

GC content: 49.71

Gene sequence:

>1362_bases
ATGAGTGACTCAAAGGGCGAGCTGCTCCGTGGGCTTGTTTCAGTGGAAGCAGAACAAAGTGTATTGGGCGGTCTCATGCT
TGATAACGATCGCTGGGATGATATTGCCCTCATTCTCAGTGTTGAGGATTTCTTCAGTAAAGCCCACCAAATCTACTTTC
GTGAGATGAAACGCCTCATTGGCGCTGGGAAACCTATTGATTTACTGACGCTAACTGATTTTTTGAAGCAGCAGGAGCAA
CTGGAGTTGGTTGGTGGTTTCGCCTATGCCGCAGAACTCAGTAAGAACACCCCCAGTGCCGCCAATATTGTGGCTTACAG
TGAAATTGTTGCCCGTTTCAGCCGTGCCCGTCAACTTGTGACCTTAGGCAGTGATATCACTCGTGAGGCCACCGCACCCC
GTGCGGATATTGCTGAAATTATGGAAAAAGCTGAAAAACGGATCATCGATATTGCCGAAAAATCTGAGCCTGAAAAGGCC
GTATCTCTTATTGAGGGGATGGAAAGATTAATTACGCAGCTTGAACTGCGAAATCAGTCGGGCAACGGTATAACCGGTAC
CCCTACTGGGTTTGTTGAGCTTGATGCCAGCACCTGTGGCCTTCAAAACAGTGATTTGATTTTGCTGGCTGGCCGCCCAT
CAATGGGTAAAACGGCGTTAGCCATGTCGATGGTTGTTGGCGCGCTTCAGGGACGAGAAGGCAGTGTGGTGCAGGTGTAT
AGCCTTGAGCAGCCCACCGAACAGTTATTGATGCGCATGATTTCTTCTCTTGGACGTATTGAGCTTCAGCGCCTTAAGAG
TGGTTTGCTGGATGATGAGGATTGGGCTCGCATAAGTCATGCTTCCAGCATTATGGTTGGAGAATGGCGAGACAGATTAA
TTATTGATGATACCGGCAGCCTGACGCCCGCCATGCTGCGAATACGCGCTCGTCGTAATGCGCGCAAGAATGGCCCGCCC
GCGTTGATTATGTTGGATTATTTACAACTGATGCGTTGCCCAAGACAGGAGAACCGTACGCAGGAGATTGCTGAGATTTC
CCGCTCTCTAAAAGCGCTGGCAAAAGAAATGAAATGCCCTGTACTGGCGCTCTCTCAACTTAATCGTTCTCTGGAACAGC
GTGCTGATAAGCGCCCTAATAATGGTGATCTTCGTGACTCTGGCGCACTGGAACAAGATGCTGACGTCATTATGTTTGTC
TATCGCGATGAGGTCTATAACGTCAGTACTGAAGATCAGGGGATCGCTGAAATCATTATCGGCAAGCAGCGCCAGGGGCC
GATTGGTACGGTCAGAGTGAGGTTTGACAGCCGTTACACCCGTTTCGACGATATCAAGAGTTGGGGGCCTGCTGATGAGT
AA

Upstream 100 bases:

>100_bases
GACTCCGCATTCGTGACCATGCATAGCCTCGCCAGCGAGCTTTTCCCTGAGTGGCGAAACAATTTCGACAGGTTACAGTC
CCCTGCAAAAGGGGTTGATC

Downstream 100 bases:

>100_bases
AATCAATGGGTTAAATCTGTCAGAGGCGATGCTGCAGCGCGGTAGACTGCCCCACTCAGTCGGGCACCCAACGGCTCTAC
CGGTTAGCGAAATGGCCATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSDSKGELLRGLVSVEAEQSVLGGLMLDNDRWDDIALILSVEDFFSKAHQIYFREMKRLIGAGKPIDLLTLTDFLKQQEQ
LELVGGFAYAAELSKNTPSAANIVAYSEIVARFSRARQLVTLGSDITREATAPRADIAEIMEKAEKRIIDIAEKSEPEKA
VSLIEGMERLITQLELRNQSGNGITGTPTGFVELDASTCGLQNSDLILLAGRPSMGKTALAMSMVVGALQGREGSVVQVY
SLEQPTEQLLMRMISSLGRIELQRLKSGLLDDEDWARISHASSIMVGEWRDRLIIDDTGSLTPAMLRIRARRNARKNGPP
ALIMLDYLQLMRCPRQENRTQEIAEISRSLKALAKEMKCPVLALSQLNRSLEQRADKRPNNGDLRDSGALEQDADVIMFV
YRDEVYNVSTEDQGIAEIIIGKQRQGPIGTVRVRFDSRYTRFDDIKSWGPADE

Sequences:

>Translated_453_residues
MSDSKGELLRGLVSVEAEQSVLGGLMLDNDRWDDIALILSVEDFFSKAHQIYFREMKRLIGAGKPIDLLTLTDFLKQQEQ
LELVGGFAYAAELSKNTPSAANIVAYSEIVARFSRARQLVTLGSDITREATAPRADIAEIMEKAEKRIIDIAEKSEPEKA
VSLIEGMERLITQLELRNQSGNGITGTPTGFVELDASTCGLQNSDLILLAGRPSMGKTALAMSMVVGALQGREGSVVQVY
SLEQPTEQLLMRMISSLGRIELQRLKSGLLDDEDWARISHASSIMVGEWRDRLIIDDTGSLTPAMLRIRARRNARKNGPP
ALIMLDYLQLMRCPRQENRTQEIAEISRSLKALAKEMKCPVLALSQLNRSLEQRADKRPNNGDLRDSGALEQDADVIMFV
YRDEVYNVSTEDQGIAEIIIGKQRQGPIGTVRVRFDSRYTRFDDIKSWGPADE
>Mature_452_residues
SDSKGELLRGLVSVEAEQSVLGGLMLDNDRWDDIALILSVEDFFSKAHQIYFREMKRLIGAGKPIDLLTLTDFLKQQEQL
ELVGGFAYAAELSKNTPSAANIVAYSEIVARFSRARQLVTLGSDITREATAPRADIAEIMEKAEKRIIDIAEKSEPEKAV
SLIEGMERLITQLELRNQSGNGITGTPTGFVELDASTCGLQNSDLILLAGRPSMGKTALAMSMVVGALQGREGSVVQVYS
LEQPTEQLLMRMISSLGRIELQRLKSGLLDDEDWARISHASSIMVGEWRDRLIIDDTGSLTPAMLRIRARRNARKNGPPA
LIMLDYLQLMRCPRQENRTQEIAEISRSLKALAKEMKCPVLALSQLNRSLEQRADKRPNNGDLRDSGALEQDADVIMFVY
RDEVYNVSTEDQGIAEIIIGKQRQGPIGTVRVRFDSRYTRFDDIKSWGPADE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=436, Percent_Identity=53.2110091743119, Blast_Score=445, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50431; Mature: 50300

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSKGELLRGLVSVEAEQSVLGGLMLDNDRWDDIALILSVEDFFSKAHQIYFREMKRLI
CCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
GAGKPIDLLTLTDFLKQQEQLELVGGFAYAAELSKNTPSAANIVAYSEIVARFSRARQLV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TLGSDITREATAPRADIAEIMEKAEKRIIDIAEKSEPEKAVSLIEGMERLITQLELRNQS
HHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
GNGITGTPTGFVELDASTCGLQNSDLILLAGRPSMGKTALAMSMVVGALQGREGSVVQVY
CCCCCCCCCCEEEECCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
SLEQPTEQLLMRMISSLGRIELQRLKSGLLDDEDWARISHASSIMVGEWRDRLIIDDTGS
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCEEEECCCC
LTPAMLRIRARRNARKNGPPALIMLDYLQLMRCPRQENRTQEIAEISRSLKALAKEMKCP
CCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCH
VLALSQLNRSLEQRADKRPNNGDLRDSGALEQDADVIMFVYRDEVYNVSTEDQGIAEIII
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHC
GKQRQGPIGTVRVRFDSRYTRFDDIKSWGPADE
CCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
SDSKGELLRGLVSVEAEQSVLGGLMLDNDRWDDIALILSVEDFFSKAHQIYFREMKRLI
CCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
GAGKPIDLLTLTDFLKQQEQLELVGGFAYAAELSKNTPSAANIVAYSEIVARFSRARQLV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
TLGSDITREATAPRADIAEIMEKAEKRIIDIAEKSEPEKAVSLIEGMERLITQLELRNQS
HHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
GNGITGTPTGFVELDASTCGLQNSDLILLAGRPSMGKTALAMSMVVGALQGREGSVVQVY
CCCCCCCCCCEEEECCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
SLEQPTEQLLMRMISSLGRIELQRLKSGLLDDEDWARISHASSIMVGEWRDRLIIDDTGS
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCEEEECCCC
LTPAMLRIRARRNARKNGPPALIMLDYLQLMRCPRQENRTQEIAEISRSLKALAKEMKCP
CCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCH
VLALSQLNRSLEQRADKRPNNGDLRDSGALEQDADVIMFVYRDEVYNVSTEDQGIAEIII
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCHHHHHC
GKQRQGPIGTVRVRFDSRYTRFDDIKSWGPADE
CCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]