Click here to switch to the map view.

The map label for this gene is lplA

Identifier: 153948776

GI number: 153948776

Start: 2001098

End: 2002114

Strand: Direct

Name: lplA

Synonym: YpsIP31758_1733

Alternate gene names: 153948776

Gene position: 2001098-2002114 (Clockwise)

Preceding gene: 153948338

Following gene: 153948973

Centisome position: 42.37

GC content: 45.03

Gene sequence:

>1017_bases
ATGTCATCCCTTCGTTTACTCATTTCTGATTCTTATGATCCTTGGTTTAATCTGGCCGTAGAAGAGTGTATTTTTCGCCA
AATGTCACCGAATCAACGGGTATTATTCTTGTGGCGCAATGCGGATACCGTGGTTATTGGCCGCGCACAGAACCCGTGGA
AAGAGTGTAATACACGACGTATGGAACAAGATGGCGTAAAGTTAGCGCGCCGTAGCAGTGGTGGCGGGGCGGTATTTCAT
GATTTAGGTAATACCTGTTTTACCTTTATGGCCGGCAAACCGGGATATGATAAAACTATTTCAACCCAAATTATTTTGAA
TGCGTTGGCATCATTAGGGATCCAGGCAACGGCCTCAGGTCGCAATGATTTAGTGGTTATTAATGGTGAAGATGAACGTA
AAGTTTCAGGTTCTGCTTATAAAGAAACCAAAGATCGTGGTTTTCATCATGGAACACTATTATTGAATGCAGATCTCAGT
CGCCTGGCTGATTATCTCAATCCAGACCCTAAAAAGTTACAGGCTAAAGGAATTACCTCGGTACGCTCTAGAGTCACTAA
TCTGGTCGAACTGTTACCGGGTATTGATCACGGAAAAATACGTACCGCTATCGAACAGGCTTTTTTTGCTTATTACGATG
AGCAGGTATCCGCAGAAGTCATCTCTCCGCAGTCGTTACCAAACCTCCCTGGCTTTACCGAGCAATTCGCCAAACAAAGT
AGTTGGGAATGGAATTTTGGCCAAGCCCCTGCATTTAGCCACGTGGTTGATACCCGCTTTATCTGGGGGGGTATTGAGTT
ACATTTTGATGTTTTGCATGGCGCAATAGATCGCTGCCAAATATTTACTGACAGCTTGAACCCCACACCATTGGAGGCGT
TAGCGCAACGGCTACAGGGGGCAGCTTATCGCCCTGATGCTATCGATAAAATCTGTCAGCACTGGATTGATGATTTCCCT
GAATTACAAACCGAGTTACAGCAAGCCTGCCATTGGCTGGTAGAAGTATTACGCTAA

Upstream 100 bases:

>100_bases
AATTTTAGTTGACGACGACAGTCCCTCTCTTCTCGGCCTAAGGATATCATCAGCGAATATGCTATCTTAGTGGCTGTTTA
TTTTTGGGTCGGAGCCTTCT

Downstream 100 bases:

>100_bases
TCTTTCAATTACCGTGGTTATTGGCCGCGCACAGCACTCGTGGACATAGAGATGAAACCCAGAATGGAATACGCCCCTGA
ATTCGATAACAGCTATGCGC

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSSLRLLISDSYDPWFNLAVEECIFRQMSPNQRVLFLWRNADTVVIGRAQNPWKECNTRRMEQDGVKLARRSSGGGAVFH
DLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASGRNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLS
RLADYLNPDPKKLQAKGITSVRSRVTNLVELLPGIDHGKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
SWEWNFGQAPAFSHVVDTRFIWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQGAAYRPDAIDKICQHWIDDFP
ELQTELQQACHWLVEVLR

Sequences:

>Translated_338_residues
MSSLRLLISDSYDPWFNLAVEECIFRQMSPNQRVLFLWRNADTVVIGRAQNPWKECNTRRMEQDGVKLARRSSGGGAVFH
DLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASGRNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLS
RLADYLNPDPKKLQAKGITSVRSRVTNLVELLPGIDHGKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
SWEWNFGQAPAFSHVVDTRFIWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQGAAYRPDAIDKICQHWIDDFP
ELQTELQQACHWLVEVLR
>Mature_337_residues
SSLRLLISDSYDPWFNLAVEECIFRQMSPNQRVLFLWRNADTVVIGRAQNPWKECNTRRMEQDGVKLARRSSGGGAVFHD
LGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASGRNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLSR
LADYLNPDPKKLQAKGITSVRSRVTNLVELLPGIDHGKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQSS
WEWNFGQAPAFSHVVDTRFIWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQGAAYRPDAIDKICQHWIDDFPE
LQTELQQACHWLVEVLR

Specific function: Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family

Homologues:

Organism=Homo sapiens, GI7706252, Length=283, Percent_Identity=33.9222614840989, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21729884, Length=283, Percent_Identity=33.9222614840989, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21729882, Length=283, Percent_Identity=33.9222614840989, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21729880, Length=283, Percent_Identity=33.9222614840989, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21729878, Length=283, Percent_Identity=33.9222614840989, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1790846, Length=338, Percent_Identity=74.8520710059172, Blast_Score=536, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI71981909, Length=263, Percent_Identity=29.6577946768061, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6322415, Length=194, Percent_Identity=31.9587628865979, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28573464, Length=262, Percent_Identity=33.206106870229, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPLA_YERP3 (A7FHI0)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400708.1
- ProteinModelPortal:   A7FHI0
- SMR:   A7FHI0
- STRING:   A7FHI0
- GeneID:   5388012
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1733
- eggNOG:   COG0095
- HOGENOM:   HBG715377
- OMA:   YEQSHLE
- ProtClustDB:   PRK03822
- BioCyc:   YPSE349747:YPSIP31758_1733-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01602
- InterPro:   IPR004143
- InterPro:   IPR005107
- InterPro:   IPR019491
- InterPro:   IPR004562
- Gene3D:   G3DSA:3.30.390.50
- TIGRFAMs:   TIGR00545

Pfam domain/function: PF03099 BPL_LipA_LipB; PF10437 Lip_prot_lig_C

EC number: =2.7.7.63

Molecular weight: Translated: 37994; Mature: 37863

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: BINDING 71-71 BINDING 134-134 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLRLLISDSYDPWFNLAVEECIFRQMSPNQRVLFLWRNADTVVIGRAQNPWKECNTRR
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHH
MEQDGVKLARRSSGGGAVFHDLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASG
HHHHHHHHHHCCCCCCCHHHHCCCHHEEHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCC
RNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLSRLADYLNPDPKKLQAKGITS
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHH
VRSRVTNLVELLPGIDHGKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
SWEWNFGQAPAFSHVVDTRFIWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQG
CCCCCCCCCCCHHHHHHHHHEECCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCC
AAYRPDAIDKICQHWIDDFPELQTELQQACHWLVEVLR
CCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SSLRLLISDSYDPWFNLAVEECIFRQMSPNQRVLFLWRNADTVVIGRAQNPWKECNTRR
CCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCHHHHHHHH
MEQDGVKLARRSSGGGAVFHDLGNTCFTFMAGKPGYDKTISTQIILNALASLGIQATASG
HHHHHHHHHHCCCCCCCHHHHCCCHHEEHCCCCCCCCCHHHHHHHHHHHHHCCCEEECCC
RNDLVVINGEDERKVSGSAYKETKDRGFHHGTLLLNADLSRLADYLNPDPKKLQAKGITS
CCCEEEECCCCCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHHH
VRSRVTNLVELLPGIDHGKIRTAIEQAFFAYYDEQVSAEVISPQSLPNLPGFTEQFAKQS
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
SWEWNFGQAPAFSHVVDTRFIWGGIELHFDVLHGAIDRCQIFTDSLNPTPLEALAQRLQG
CCCCCCCCCCCHHHHHHHHHEECCHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHCC
AAYRPDAIDKICQHWIDDFPELQTELQQACHWLVEVLR
CCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA