Click here to switch to the map view.

The map label for this gene is sufS

Identifier: 153947303

GI number: 153947303

Start: 2019270

End: 2020490

Strand: Direct

Name: sufS

Synonym: YpsIP31758_1745

Alternate gene names: 153947303

Gene position: 2019270-2020490 (Clockwise)

Preceding gene: 153947648

Following gene: 153950798

Centisome position: 42.75

GC content: 51.27

Gene sequence:

>1221_bases
ATGAGTTTTCCTATTGAGCGTGTAAGAGCTGATTTTCCACTGTTGAGCCGCCAGGTTAATGGGCAGCCGTTGGTTTATCT
GGACAGCGCCGCCAGTGCGCAAAAACCTCAGGCGGTCATTGACAAGGAGCTTCATTTTTACCGTGATGGTTATGCGGCCG
TTCATCGGGGCATTCACAGTTTAAGTGCTGAAGCGACTCAGCAAATGGAAGCAGTACGCACTCAGGTGGCTGATTTTATT
CACGCCGCATCCGCAGAAGAAATTATCTTTGTCCGAGGCACCACTGAAGCAATCAATTTGGTTGCTAACAGTTATGGCCG
CCATTTCCTTGTCACGGGTGATAGCATTATCATTACCGAAATGGAACATCATGCCAATATTGTGCCTTGGCAGATGTTGG
CGCAAGATCTCGGTGTTGAAATCCGTGTTTGGCCACTGACGGCTACCGGTGAGTTAGAGATAACCGACTTGGCAGCGTTG
ATTGATGACACCACGCGCTTACTGGCGGTGACTCAGATCTCCAACGTGTTGGGAACGGTAAACCCGATTAAGGATATTGT
GGCTCAGGCAAAAGCTGCTGGTTTGGTGGTGCTGGTGGATGGTGCGCAAGCGGTTATGCATCAGCCAGTTGATGTTCAGG
CGTTGGGCTGCGATTTTTATGTTTTCTCAGGGCACAAACTGTACGGCCCATCGGGTATTGGGATTCTGTACGGCAAAAGT
GCGTTGTTACAACAGATGCCGCCATGGGAAGGGGGCGGGGCGATGATCAAAACAGTCAGTTTGACGCAAGGCACTACGTT
TGCTGATGCCCCTTGGCGCTTTGAGGCTGGGTCACCTAATACTGCGGGGATCATGGGGCTTGGCGCGGCCATTGACTATG
TCACTGAATTAGGGCTCTTGCAGATCCAACAGTATGAACAATCGCTGATGCATTACGCATTGGCGCAACTGAGCCAGATT
AAGAGCCTGACACTGTATGGCCCAACAGAGCGTGCCGGGGTTATTGCCTTCAATCTGGGCCTGCACCATGCCTATGATGT
GGGCAGCTTTCTTGACCAATACGGTATTGCTATTCGTACGGGTCATCACTGTGCGATGCCGCTGATGGCATTCTATCAGG
TACCGAGTATGTGCCGTGCCTCACTGGCGCTGTATAATACCCGCGAGGATGTTGATCGGTTGGTGGCAGGATTACAGCGT
ATCGAAAAATTGCTGGGGTGA

Upstream 100 bases:

>100_bases
TTTTTGCGTTTGCTGCTGAAGTGACTGAAGCTATTCACAACGACGTCATCCGTAAACAGGTATTAGCACGTATTGCTGAA
CGTTTGGCGTGGGAGTCCTT

Downstream 100 bases:

>100_bases
GAGTCTAAGCACCCACGACAGGCAATACCAAAGCCGACTATTCTTTCAGCAGTCGGCTTTTATTTTTTACGTATTCAGGA
AATATGCTATGGCTGGTTTG

Product: bifunctional cysteine desulfurase/selenocysteine lyase

Products: NA

Alternate protein names: Selenocysteine beta-lyase; SCL; Selenocysteine lyase; Selenocysteine reductase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSFPIERVRADFPLLSRQVNGQPLVYLDSAASAQKPQAVIDKELHFYRDGYAAVHRGIHSLSAEATQQMEAVRTQVADFI
HAASAEEIIFVRGTTEAINLVANSYGRHFLVTGDSIIITEMEHHANIVPWQMLAQDLGVEIRVWPLTATGELEITDLAAL
IDDTTRLLAVTQISNVLGTVNPIKDIVAQAKAAGLVVLVDGAQAVMHQPVDVQALGCDFYVFSGHKLYGPSGIGILYGKS
ALLQQMPPWEGGGAMIKTVSLTQGTTFADAPWRFEAGSPNTAGIMGLGAAIDYVTELGLLQIQQYEQSLMHYALAQLSQI
KSLTLYGPTERAGVIAFNLGLHHAYDVGSFLDQYGIAIRTGHHCAMPLMAFYQVPSMCRASLALYNTREDVDRLVAGLQR
IEKLLG

Sequences:

>Translated_406_residues
MSFPIERVRADFPLLSRQVNGQPLVYLDSAASAQKPQAVIDKELHFYRDGYAAVHRGIHSLSAEATQQMEAVRTQVADFI
HAASAEEIIFVRGTTEAINLVANSYGRHFLVTGDSIIITEMEHHANIVPWQMLAQDLGVEIRVWPLTATGELEITDLAAL
IDDTTRLLAVTQISNVLGTVNPIKDIVAQAKAAGLVVLVDGAQAVMHQPVDVQALGCDFYVFSGHKLYGPSGIGILYGKS
ALLQQMPPWEGGGAMIKTVSLTQGTTFADAPWRFEAGSPNTAGIMGLGAAIDYVTELGLLQIQQYEQSLMHYALAQLSQI
KSLTLYGPTERAGVIAFNLGLHHAYDVGSFLDQYGIAIRTGHHCAMPLMAFYQVPSMCRASLALYNTREDVDRLVAGLQR
IEKLLG
>Mature_405_residues
SFPIERVRADFPLLSRQVNGQPLVYLDSAASAQKPQAVIDKELHFYRDGYAAVHRGIHSLSAEATQQMEAVRTQVADFIH
AASAEEIIFVRGTTEAINLVANSYGRHFLVTGDSIIITEMEHHANIVPWQMLAQDLGVEIRVWPLTATGELEITDLAALI
DDTTRLLAVTQISNVLGTVNPIKDIVAQAKAAGLVVLVDGAQAVMHQPVDVQALGCDFYVFSGHKLYGPSGIGILYGKSA
LLQQMPPWEGGGAMIKTVSLTQGTTFADAPWRFEAGSPNTAGIMGLGAAIDYVTELGLLQIQQYEQSLMHYALAQLSQIK
SLTLYGPTERAGVIAFNLGLHHAYDVGSFLDQYGIAIRTGHHCAMPLMAFYQVPSMCRASLALYNTREDVDRLVAGLQRI
EKLLG

Specific function: Cysteine desulfurases mobilize the sulfur from L- cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under speci

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=298, Percent_Identity=27.1812080536913, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI156713448, Length=398, Percent_Identity=26.1306532663317, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1787970, Length=406, Percent_Identity=69.9507389162562, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1789175, Length=404, Percent_Identity=44.0594059405941, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI48994898, Length=215, Percent_Identity=31.1627906976744, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=216, Percent_Identity=30.0925925925926, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI193211090, Length=382, Percent_Identity=24.869109947644, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319831, Length=233, Percent_Identity=30.9012875536481, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129463, Length=242, Percent_Identity=30.1652892561983, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SUFS_YERP3 (A7FHJ2)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400720.1
- ProteinModelPortal:   A7FHJ2
- SMR:   A7FHJ2
- STRING:   A7FHJ2
- GeneID:   5386057
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1745
- NMPDR:   fig|349747.3.peg.792
- eggNOG:   COG0520
- HOGENOM:   HBG635316
- OMA:   EEDNANV
- ProtClustDB:   PRK09295
- BioCyc:   YPSE349747:YPSIP31758_1745-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01831
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR01979

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7; =4.4.1.16

Molecular weight: Translated: 44130; Mature: 43999

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 364-364

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFPIERVRADFPLLSRQVNGQPLVYLDSAASAQKPQAVIDKELHFYRDGYAAVHRGIHS
CCCCHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LSAEATQQMEAVRTQVADFIHAASAEEIIFVRGTTEAINLVANSYGRHFLVTGDSIIITE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCEEEEECCEEEEEE
MEHHANIVPWQMLAQDLGVEIRVWPLTATGELEITDLAALIDDTTRLLAVTQISNVLGTV
EHHCCCCCCHHHHHHHCCCEEEEEEEECCCCEEHHHHHHHHHCHHHHHHHHHHHHHHCCC
NPIKDIVAQAKAAGLVVLVDGAQAVMHQPVDVQALGCDFYVFSGHKLYGPSGIGILYGKS
HHHHHHHHHHHHCCEEEEECCCHHHHCCCCCEEEEEEEEEEEECCEEECCCCCEEEECCH
ALLQQMPPWEGGGAMIKTVSLTQGTTFADAPWRFEAGSPNTAGIMGLGAAIDYVTELGLL
HHHHCCCCCCCCCCEEEEEEEECCCEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
QIQQYEQSLMHYALAQLSQIKSLTLYGPTERAGVIAFNLGLHHAYDVGSFLDQYGIAIRT
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEC
GHHCAMPLMAFYQVPSMCRASLALYNTREDVDRLVAGLQRIEKLLG
CCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SFPIERVRADFPLLSRQVNGQPLVYLDSAASAQKPQAVIDKELHFYRDGYAAVHRGIHS
CCCHHHHHCCCCHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LSAEATQQMEAVRTQVADFIHAASAEEIIFVRGTTEAINLVANSYGRHFLVTGDSIIITE
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCCCEEEEECCEEEEEE
MEHHANIVPWQMLAQDLGVEIRVWPLTATGELEITDLAALIDDTTRLLAVTQISNVLGTV
EHHCCCCCCHHHHHHHCCCEEEEEEEECCCCEEHHHHHHHHHCHHHHHHHHHHHHHHCCC
NPIKDIVAQAKAAGLVVLVDGAQAVMHQPVDVQALGCDFYVFSGHKLYGPSGIGILYGKS
HHHHHHHHHHHHCCEEEEECCCHHHHCCCCCEEEEEEEEEEEECCEEECCCCCEEEECCH
ALLQQMPPWEGGGAMIKTVSLTQGTTFADAPWRFEAGSPNTAGIMGLGAAIDYVTELGLL
HHHHCCCCCCCCCCEEEEEEEECCCEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHH
QIQQYEQSLMHYALAQLSQIKSLTLYGPTERAGVIAFNLGLHHAYDVGSFLDQYGIAIRT
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEC
GHHCAMPLMAFYQVPSMCRASLALYNTREDVDRLVAGLQRIEKLLG
CCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA