Click here to switch to the map view.

The map label for this gene is mltF [H]

Identifier: 153947131

GI number: 153947131

Start: 1345443

End: 1346903

Strand: Reverse

Name: mltF [H]

Synonym: YpsIP31758_1146

Alternate gene names: 153947131

Gene position: 1346903-1345443 (Counterclockwise)

Preceding gene: 153950100

Following gene: 153950128

Centisome position: 28.52

GC content: 49.49

Gene sequence:

>1461_bases
TTGACACGCATAAAATTAAGTTACTTTACCATCGGTCTGGTTGCTCTGCTTCTGGCGCTTGCGCTGTGGCCCAATATTCC
TTGGCGTAATGGGCAAGAGGGCCAACTTGACCAAATAAAAGCCCGTGGAGAACTGCGCGTCAGTACCATCAGTTCTCCTT
TGATCTACTCTACCGAAAAGGATACCCCTTCCGGTTTTGACTATGAGCTAGCAAAACGTTTTGCTGACTATCTGGGGGTT
AAACTGGTTATCATCCCCCATCATAATATTGACGATCTGTTTGACGCATTAGATAACGATGACACCGATCTGCTAGCCGC
TGGGCTGATTTATAACCGTGAACGCCTCAATCGGGCGCGGACTGGCCCTGCCTACTATTCTGTTTCTCAGCAATTGGTCT
ACCGGCTGGGTTCGCCACGGCCTAAATCGTTCAGCGATCTGAAAGGTCAGGTGGTTGTGGCCTCCGGTTCGGCCCATATG
ACCACACTAAAACGGCTTAAGCAGACAAAATATCCTGAACTCAATTGGAGTTCATCCGTCGATAAGTCCGGTAAAGAATT
ACTGGAGCAAGTCGCGGAAGGTAAGCTCGACTATACCCTTGGCGATTCAGCCACCATTGCCCTGTTACAACGCATTCATC
CGCAATTGGCAGTCGCATTTGATGTCACCGATGAAGAGCCCGTCACCTGGTATTTTAAACAGAGTGATGACGATAGCCTG
TATGCGGCCATGCTCGATTTCTACAGTGAGATGGTGGAAGACGGTAGCCTCGCACGTCTGGAGGAGAAATATCTGGGCCA
TGTGGGGAGTTTTGACTATGTCGATACCAAAACTTTCCTGTCCGCTATCGATAATGTCCTGCCTTCTTACCAGCACCTAT
TTGAAAAACATGCAGGGGACATTGACTGGAAATTGCTGGCCGTGATTGCCTATCAAGAATCACATTGGAACCCGCAAGCC
ACCTCCCCTACTGGTGTTCGTGGCTTGATGATGCTAACCCGTGCGACCGCGGACGGGTTAGGGGTCAAGGATAGGGTTGA
CCCTGAAGAAAGCATCAGAGGCGGGGCTATCTATTTGCAACGGCTGATGAAAAAACTCCCTGAAACCATCCCAGAAGATG
AGCGCATCTGGTTTGCACTGGCTGCGTATAATTTAGGCTATGGTCATATGCTGGATGCCCGCCGTTTGACTAAAAATCAG
AACGGTAACCCAGACAGTTGGGTTGATGTGAAAATGCGTTTACCGATGCTAAGCCAAAAACGCTACTACCCAAGCACCAC
TTACGGTTATGCCCGTGGTCATGAAGCCTATAACTATGTCGAGAATATTCGCCGTTATCAGGTCAGTCTGGTGGGATATT
TACAGGAAAAAGAGAAAAAAGCGGCTCAACATGCCGCCATTGAGGCGGAGCTGGGCAAAAGCAACCCGGTTGTCGGCCCC
GGTTGGTCAATCGGTGACTAA

Upstream 100 bases:

>100_bases
TGGCGTTTTGTTGCGCAAAATGCCACTCCACTGGGGATTTAACCATGTTGAATTTAGCTTTACCGCACACACGAAGAGAG
CGTTTCCGAGAGATAACTAT

Downstream 100 bases:

>100_bases
TCGCTAGCCAGTAATTCGTCTGTTATCAGTCACTCGTCGGCAGTCGTTCAACCTTAATTACACCGTGATTTAGTCACCAA
CCGCCGCCCGTCATAACATG

Product: transglycosylase

Products: NA

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGV
KLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVVASGSAHM
TTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIHPQLAVAFDVTDEEPVTWYFKQSDDDSL
YAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQA
TSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGHMLDARRLTKNQ
NGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGYLQEKEKKAAQHAAIEAELGKSNPVVGP
GWSIGD

Sequences:

>Translated_486_residues
MTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGV
KLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVVASGSAHM
TTLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIHPQLAVAFDVTDEEPVTWYFKQSDDDSL
YAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQA
TSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGHMLDARRLTKNQ
NGNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGYLQEKEKKAAQHAAIEAELGKSNPVVGP
GWSIGD
>Mature_485_residues
TRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSPLIYSTEKDTPSGFDYELAKRFADYLGVK
LVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRARTGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVVASGSAHMT
TLKRLKQTKYPELNWSSSVDKSGKELLEQVAEGKLDYTLGDSATIALLQRIHPQLAVAFDVTDEEPVTWYFKQSDDDSLY
AAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHLFEKHAGDIDWKLLAVIAYQESHWNPQAT
SPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQRLMKKLPETIPEDERIWFALAAYNLGYGHMLDARRLTKNQN
GNPDSWVDVKMRLPMLSQKRYYPSTTYGYARGHEAYNYVENIRRYQVSLVGYLQEKEKKAAQHAAIEAELGKSNPVVGPG
WSIGD

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=481, Percent_Identity=60.9147609147609, Blast_Score=597, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: NA

Molecular weight: Translated: 54809; Mature: 54677

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSPLIYSTEK
CCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCEEEECCC
DTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRAR
CCCCCCCHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
TGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
DKSGKELLEQVAEGKLDYTLGDSATIALLQRIHPQLAVAFDVTDEEPVTWYFKQSDDDSL
CHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCHH
YAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHLFEKHAGD
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
IDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQ
CCEEEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHH
RLMKKLPETIPEDERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQK
HHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCHHHCC
RYYPSTTYGYARGHEAYNYVENIRRYQVSLVGYLQEKEKKAAQHAAIEAELGKSNPVVGP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GWSIGD
CCCCCC
>Mature Secondary Structure 
TRIKLSYFTIGLVALLLALALWPNIPWRNGQEGQLDQIKARGELRVSTISSPLIYSTEK
CEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCEEEECCC
DTPSGFDYELAKRFADYLGVKLVIIPHHNIDDLFDALDNDDTDLLAAGLIYNRERLNRAR
CCCCCCCHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
TGPAYYSVSQQLVYRLGSPRPKSFSDLKGQVVVASGSAHMTTLKRLKQTKYPELNWSSSV
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCC
DKSGKELLEQVAEGKLDYTLGDSATIALLQRIHPQLAVAFDVTDEEPVTWYFKQSDDDSL
CHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCHH
YAAMLDFYSEMVEDGSLARLEEKYLGHVGSFDYVDTKTFLSAIDNVLPSYQHLFEKHAGD
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
IDWKLLAVIAYQESHWNPQATSPTGVRGLMMLTRATADGLGVKDRVDPEESIRGGAIYLQ
CCEEEEEEEEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHH
RLMKKLPETIPEDERIWFALAAYNLGYGHMLDARRLTKNQNGNPDSWVDVKMRLPMLSQK
HHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCHHHCC
RYYPSTTYGYARGHEAYNYVENIRRYQVSLVGYLQEKEKKAAQHAAIEAELGKSNPVVGP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GWSIGD
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA