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The map label for this gene is murD [H]

Identifier: 153952454

GI number: 153952454

Start: 1337436

End: 1338644

Strand: Direct

Name: murD [H]

Synonym: JJD26997_1512

Alternate gene names: 153952454

Gene position: 1337436-1338644 (Clockwise)

Preceding gene: 153951175

Following gene: 153952063

Centisome position: 72.49

GC content: 31.18

Gene sequence:

>1209_bases
ATGAAAATTTCACTTTTTGGATACGGAAAAACCACATGTTCTATTGCTGAAAATTTAGTAGATAAGTTTGGGCCTTTTGA
TATCTATGATGATCATTTTACAGAAACAAAAAAAGATACACTAGGGAATTTACTTTTAAATCCTAATGATTTTGATGATA
ATTTAAGCAATATTGAAATTCCTAGTCCAGGTTTTCCACCAAACCACAAACTCATTCAAGAAGCAAAAAATTTACAAAGT
GAATATGACTTTTTTTACGATGTCATGCCAAAATCCGTTTGGATAAGCGGAACCAATGGCAAAACCACCACCACTCAAAT
GGCGATGCATTTATTATCACACATTGGTGCTGTTATGGGTGGAAATGTTGGAACTCCTCTAGCAGAACTTGATCCTTATG
CAAAACTTTGGATACTTGAAACTTCATCTTTTACCCTACACTACACTCATAAAGCTAAACCTGAAATTTATGCACTTTTA
CCCATAAGCCCTGATCATCTTTCTTGGCATGGAAGTTTTAAAGATTATATTCAAGATAAACTAAGCGTTTTAAAAAGAAT
GAATGAATGTGATGTAGCAATACTACCCAAAATTCATACAAATACTCCAACAAAAGCTCATATAATAAGCTATGAAGATG
AAAAAGATCTAGCTACTAAGTTTAGCATTGATACGGAAAAAATTTCCTTTAAAAGTCCTTTTTTACTCGATGCTATCATG
GCTTTAGCTATAGAAAAAATTCTACTTGATACTTTAAGTTATAAGCTTTTAAATAACTTTGTAATGGAAAAAAATAAACT
TGAAGAGATTAAGGATAGTCAAAATAGACTTTGGGTAAATGACACTAAAGCCACAAATGAAAGTGCTGTAATGGCTGCAC
TTAATCGCTATAAAGATAAAAAAATTCATCTTATCATAGGCGGAGATGATAAAGGTGTAGATCTTAGTAATTTATTTGAT
TTTATGAAAGGATTTGATATAGAACTTTACGCCATAGGTATTAGCACTGAAAAAATGCTTAATTATGCAAAAAAGGCAAA
TTTAAAAGCTTATAAATGTGAAATCTTACAAAGGGCTGTAAGTGAAATTTCAAATCATTTAAAAGTCAATGAAGTAGCTC
TTTTAAGTCCTGCTTGTGCGAGCTTGGATCAATTTAGCTCTTATGCACAACGAGGAGAAGTTTTTAAAGAGTGTATAAAA
AAACTTTAA

Upstream 100 bases:

>100_bases
TTGAAAAAGTTGGCTGGGTAGAAAATAAAATTATAGTGCGTTTTTGGATGATAGCCTTACTTTCAAATTTACTTGCACTT
GCATCAATAAAGCTAAGATA

Downstream 100 bases:

>100_bases
GCATTTTATAGCTTACTTACAAACTTGTTTTAAAAGCTTAATTTAAAATTTTAAAAAGGGTAAAATTCTTATCCGCTTTG
TATTTAAAATCAATAAGTTA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKISLFGYGKTTCSIAENLVDKFGPFDIYDDHFTETKKDTLGNLLLNPNDFDDNLSNIEIPSPGFPPNHKLIQEAKNLQS
EYDFFYDVMPKSVWISGTNGKTTTTQMAMHLLSHIGAVMGGNVGTPLAELDPYAKLWILETSSFTLHYTHKAKPEIYALL
PISPDHLSWHGSFKDYIQDKLSVLKRMNECDVAILPKIHTNTPTKAHIISYEDEKDLATKFSIDTEKISFKSPFLLDAIM
ALAIEKILLDTLSYKLLNNFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDKKIHLIIGGDDKGVDLSNLFD
FMKGFDIELYAIGISTEKMLNYAKKANLKAYKCEILQRAVSEISNHLKVNEVALLSPACASLDQFSSYAQRGEVFKECIK
KL

Sequences:

>Translated_402_residues
MKISLFGYGKTTCSIAENLVDKFGPFDIYDDHFTETKKDTLGNLLLNPNDFDDNLSNIEIPSPGFPPNHKLIQEAKNLQS
EYDFFYDVMPKSVWISGTNGKTTTTQMAMHLLSHIGAVMGGNVGTPLAELDPYAKLWILETSSFTLHYTHKAKPEIYALL
PISPDHLSWHGSFKDYIQDKLSVLKRMNECDVAILPKIHTNTPTKAHIISYEDEKDLATKFSIDTEKISFKSPFLLDAIM
ALAIEKILLDTLSYKLLNNFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDKKIHLIIGGDDKGVDLSNLFD
FMKGFDIELYAIGISTEKMLNYAKKANLKAYKCEILQRAVSEISNHLKVNEVALLSPACASLDQFSSYAQRGEVFKECIK
KL
>Mature_402_residues
MKISLFGYGKTTCSIAENLVDKFGPFDIYDDHFTETKKDTLGNLLLNPNDFDDNLSNIEIPSPGFPPNHKLIQEAKNLQS
EYDFFYDVMPKSVWISGTNGKTTTTQMAMHLLSHIGAVMGGNVGTPLAELDPYAKLWILETSSFTLHYTHKAKPEIYALL
PISPDHLSWHGSFKDYIQDKLSVLKRMNECDVAILPKIHTNTPTKAHIISYEDEKDLATKFSIDTEKISFKSPFLLDAIM
ALAIEKILLDTLSYKLLNNFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDKKIHLIIGGDDKGVDLSNLFD
FMKGFDIELYAIGISTEKMLNYAKKANLKAYKCEILQRAVSEISNHLKVNEVALLSPACASLDQFSSYAQRGEVFKECIK
KL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=375, Percent_Identity=26.9333333333333, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 45399; Mature: 45399

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLFGYGKTTCSIAENLVDKFGPFDIYDDHFTETKKDTLGNLLLNPNDFDDNLSNIEI
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCEEC
PSPGFPPNHKLIQEAKNLQSEYDFFYDVMPKSVWISGTNGKTTTTQMAMHLLSHIGAVMG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
GNVGTPLAELDPYAKLWILETSSFTLHYTHKAKPEIYALLPISPDHLSWHGSFKDYIQDK
CCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHH
LSVLKRMNECDVAILPKIHTNTPTKAHIISYEDEKDLATKFSIDTEKISFKSPFLLDAIM
HHHHHHCCCCCEEEECEEECCCCCCEEEEEECCCHHHHHHCCCCHHHCCCCCCHHHHHHH
ALAIEKILLDTLSYKLLNNFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCC
KIHLIIGGDDKGVDLSNLFDFMKGFDIELYAIGISTEKMLNYAKKANLKAYKCEILQRAV
EEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHH
SEISNHLKVNEVALLSPACASLDQFSSYAQRGEVFKECIKKL
HHHHHCCEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKISLFGYGKTTCSIAENLVDKFGPFDIYDDHFTETKKDTLGNLLLNPNDFDDNLSNIEI
CEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCEEECCCCCCCCCCCEEC
PSPGFPPNHKLIQEAKNLQSEYDFFYDVMPKSVWISGTNGKTTTTQMAMHLLSHIGAVMG
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHC
GNVGTPLAELDPYAKLWILETSSFTLHYTHKAKPEIYALLPISPDHLSWHGSFKDYIQDK
CCCCCCHHHCCCCEEEEEEEECCEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHH
LSVLKRMNECDVAILPKIHTNTPTKAHIISYEDEKDLATKFSIDTEKISFKSPFLLDAIM
HHHHHHCCCCCEEEECEEECCCCCCEEEEEECCCHHHHHHCCCCHHHCCCCCCHHHHHHH
ALAIEKILLDTLSYKLLNNFVMEKNKLEEIKDSQNRLWVNDTKATNESAVMAALNRYKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCC
KIHLIIGGDDKGVDLSNLFDFMKGFDIELYAIGISTEKMLNYAKKANLKAYKCEILQRAV
EEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHH
SEISNHLKVNEVALLSPACASLDQFSSYAQRGEVFKECIKKL
HHHHHCCEECCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA