Click here to switch to the map view.

The map label for this gene is nlaIIIM [H]

Identifier: 153952442

GI number: 153952442

Start: 203490

End: 204581

Strand: Direct

Name: nlaIIIM [H]

Synonym: JJD26997_0218

Alternate gene names: 153952442

Gene position: 203490-204581 (Clockwise)

Preceding gene: 153951486

Following gene: 153951917

Centisome position: 11.03

GC content: 28.57

Gene sequence:

>1092_bases
TTGAAAGAAAATCCCTCTTTTTTAAAAGAGCAAATTATAACTTATCTTGGAAATAAAAGAGCACTTTTAAGTTTTTTAAA
TAAGGGCTTTAAGGTAGCTAAAAAAGAACTTGGTAAAGATAAATTTAGTTTTTGTGATATATTTAGTGGCTCAGGTGTTG
TAAGTCGTTTTGCAAAAGCGCACTCAAATTATATTTTAGCCAATGATTTAGAGGATTATTCAAAACTCATCAATGAGTGT
TATCTTGCTAATAAAGACAAAGATTTACTTCAAAATATAAAAAAGTATTATAAAAATTTAATCCAAAATTTAGATTTTGA
AAAAGGTTTTATCAGTGAGCTTTATGCTCCAAAAGATGATGATGATATTAAAAAAAATGAAAGGGTTTTTTATACTTTTA
AAAATGCTTTATACCTTGATACCATAAGGCAAAAAATCGAAAATGAAATTCCAAAAGAATTGAGGCATTTCTTTATAGCT
CCTTTGATTTATGAAGCCAGTGTTCATTCAAATACTAGCGGAGTTTTTAAGGGTTTTTATAAGGGTAAGGATGGCATCGG
AAAATTTGGTGGCGAAGGACAAAATGCACTTAAACGCATTAAAGGAGAAATAGAGCTTAAAATGCCTATTTTTTCAAATT
TTTCTTGTGAATTTGAAGTGATGCAAAAGGATGCAAGCGTGCTTGCAAAAGAACTTGATTTTTTTGATGTAGTGTATCTT
GACCCCCCTTATAATCAGCACCCTTATAGCTCTAATTATTTTATGCTAAATTTAATCGCAAATTATAAAAAACCTGAAGA
AATTTCTAAAATCAGTGGCATTCCAAAAGATTGGAACCGCAGTACTTTTAATAAGCCAAAATTTGCCCAAGAGGCTTTAT
TTGAACTTATTAATGATTTAAAAGCAAGAGTGATTTTACTTTCTTATAATTGTGAAGGTTTTGTAAAAAAAGAAATTTTT
TTAAAGCGCTTAAGCACTCTTGGAAAATGTCGGATTTTAGAACAAAAATATAATACTTTTAGAGCTTCTCGCAATCTTAA
AAATCGTAATATCCATCTTCAGGAACAACTTTATATTTTAGTTAAAAATTAA

Upstream 100 bases:

>100_bases
ACAAAGAGCCAAATTTTGAAGGTCGTTATGTAAATATGCTTGTAACTCCAAAAAAAGCTTAATCCACTTTTGTGGATAAG
GCTTTTAAAGGTTTTAAATT

Downstream 100 bases:

>100_bases
ATTTTGTGCTAAAATTATATTTATAAAAAGGAGGGATTTATGAAACTAAAACTATCTTTTTGTGCATTAATGGCATTTGG
TTTTTCTAACTATCTTTTTG

Product: D12 class N6 adenine-specific DNA methyltransferase

Products: NA

Alternate protein names: M.NlaIII; Adenine-specific methyltransferase NlaIII [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGVVSRFAKAHSNYILANDLEDYSKLINEC
YLANKDKDLLQNIKKYYKNLIQNLDFEKGFISELYAPKDDDDIKKNERVFYTFKNALYLDTIRQKIENEIPKELRHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEGQNALKRIKGEIELKMPIFSNFSCEFEVMQKDASVLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKPKFAQEALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLQEQLYILVKN

Sequences:

>Translated_363_residues
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGVVSRFAKAHSNYILANDLEDYSKLINEC
YLANKDKDLLQNIKKYYKNLIQNLDFEKGFISELYAPKDDDDIKKNERVFYTFKNALYLDTIRQKIENEIPKELRHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEGQNALKRIKGEIELKMPIFSNFSCEFEVMQKDASVLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKPKFAQEALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLQEQLYILVKN
>Mature_363_residues
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGVVSRFAKAHSNYILANDLEDYSKLINEC
YLANKDKDLLQNIKKYYKNLIQNLDFEKGFISELYAPKDDDDIKKNERVFYTFKNALYLDTIRQKIENEIPKELRHFFIA
PLIYEASVHSNTSGVFKGFYKGKDGIGKFGGEGQNALKRIKGEIELKMPIFSNFSCEFEVMQKDASVLAKELDFFDVVYL
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKPKFAQEALFELINDLKARVILLSYNCEGFVKKEIF
LKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLQEQLYILVKN

Specific function: This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the NlaIII endonuclease [H]

COG id: COG3392

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42296; Mature: 42296

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGVVSRFAKA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHH
HSNYILANDLEDYSKLINECYLANKDKDLLQNIKKYYKNLIQNLDFEKGFISELYAPKDD
HCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCC
DDIKKNERVFYTFKNALYLDTIRQKIENEIPKELRHFFIAPLIYEASVHSNTSGVFKGFY
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KGKDGIGKFGGEGQNALKRIKGEIELKMPIFSNFSCEFEVMQKDASVLAKELDFFDVVYL
CCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKPKFAQEALFELINDL
CCCCCCCCCCCCCEEEEEHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KARVILLSYNCEGFVKKEIFLKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLQEQLYIL
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
VKN
EEC
>Mature Secondary Structure
MKENPSFLKEQIITYLGNKRALLSFLNKGFKVAKKELGKDKFSFCDIFSGSGVVSRFAKA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHH
HSNYILANDLEDYSKLINECYLANKDKDLLQNIKKYYKNLIQNLDFEKGFISELYAPKDD
HCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCC
DDIKKNERVFYTFKNALYLDTIRQKIENEIPKELRHFFIAPLIYEASVHSNTSGVFKGFY
CCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
KGKDGIGKFGGEGQNALKRIKGEIELKMPIFSNFSCEFEVMQKDASVLAKELDFFDVVYL
CCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEE
DPPYNQHPYSSNYFMLNLIANYKKPEEISKISGIPKDWNRSTFNKPKFAQEALFELINDL
CCCCCCCCCCCCCEEEEEHHCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KARVILLSYNCEGFVKKEIFLKRLSTLGKCRILEQKYNTFRASRNLKNRNIHLQEQLYIL
HHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEE
VKN
EEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2277628 [H]