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The map label for this gene is prfB [H]

Identifier: 153952316

GI number: 153952316

Start: 1575517

End: 1576614

Strand: Direct

Name: prfB [H]

Synonym: JJD26997_1790

Alternate gene names: 153952316

Gene position: 1575517-1576614 (Clockwise)

Preceding gene: 153952384

Following gene: 153952392

Centisome position: 85.39

GC content: 37.52

Gene sequence:

>1098_bases
ATGGATAATTACGAATTTAGCGAACTTTTAAAAACTTTAAAAAACAAAGTCGGCAACATAGCCTCCATCATCAAGCCTAA
AAATATCCAAACAAGGCTTAAAGAAATCGAAGAGCTTGAAAATTCTCCTTCTTTTTGGAACGATGTCAAACAAGCAGGGA
TCATAGGAAAAGAAAAAACCAAAATCACCAATCTACTTAAAAACTATGAAAATGCTTTTAATGCTCTAAATGACGCAAGC
GAACTTTTCGATCTGGCTAATAGCGAAAACGATATAGAAACGCTTCAAGCTTTATTTAATGACGCACCAAAACTTGAAGA
TACTATCACAAGTCTTGAAATTTCCATGCTTTTAAGCGGGGAAAATGATGGTAAAAATGCCATTGTTTCCATTCACCCAG
GTGCAGGTGGAACAGAAAGTAATGACTGGGCAAGCATACTTTATAGAATGTATTTAAGATTTTGCGAAAGAGAAGGTTTT
AAAGTCGAAACCCTAGACTTTCAAGAAGGCGAAGAAGCAGGGCTTAAAGATGTGAGCTTTTTGGTTAAAGGCGAAAATGC
TTATGGTTATTTAAAGGCTGAAAATGGCATTCATCGCTTGGTAAGAACTTCTCCTTTTGATAGTGCAGGACGCCGTCATA
CAAGCTTTTCAAGTGTTATGGTAAGCCCTGAACTTGATGATGATATAGAAATTGAAATCGAAGAAAAAGACATAAGAATA
GATTATTACAGAGCAAGTGGTGCAGGCGGACAACATGTTAATAAAACAGAATCAGCCGTGAGAATCACGCATTTTCCAAC
GGGCATAGTCGTACAATGCCAAAATGACAGAAGTCAGCACAAAAACAAAGCCACCGCTTTTAAAATGCTAAAATCTCGCC
TTTATGAGCTTGAGCTTATGAAGCAGCAAGATAGTGCAAATTCAAGCGAAAAAAGCGAGATAGGCTGGGGACATCAAATC
CGCTCTTATGTGCTTTTCCCTTATCAGCAAGTTAAAGACAATCGCAGCGGAGAAGCTTTTTCTCAAGTGGATAATATCCT
TGATGGGGATATCAAAAAAATGATAGAAGGCGTTTTGATAGCTTTGAAAGCAGAGTAA

Upstream 100 bases:

>100_bases
ATTTCTTTAAACATTCGCAAAATATAAGAAATCGTGCAATAGATTTTTTAATGTAAAATTTGCTAAAATAATATCTTAAA
AATGAAGAAAGAAAATTTTT

Downstream 100 bases:

>100_bases
AATAAAGGCTAAAAAATTTAAAATGATTTTAAAAAAATTATAGGCTGATGATTCTTATTCTTTATTAGAAATTTCAATTT
TACTTGTATATTTTTGTAAC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MDNYEFSELLKTLKNKVGNIASIIKPKNIQTRLKEIEELENSPSFWNDVKQAGIIGKEKTKITNLLKNYENAFNALNDAS
ELFDLANSENDIETLQALFNDAPKLEDTITSLEISMLLSGENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGF
KVETLDFQEGEEAGLKDVSFLVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELMKQQDSANSSEKSEIGWGHQI
RSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLIALKAE

Sequences:

>Translated_365_residues
MDNYEFSELLKTLKNKVGNIASIIKPKNIQTRLKEIEELENSPSFWNDVKQAGIIGKEKTKITNLLKNYENAFNALNDAS
ELFDLANSENDIETLQALFNDAPKLEDTITSLEISMLLSGENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGF
KVETLDFQEGEEAGLKDVSFLVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELMKQQDSANSSEKSEIGWGHQI
RSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLIALKAE
>Mature_365_residues
MDNYEFSELLKTLKNKVGNIASIIKPKNIQTRLKEIEELENSPSFWNDVKQAGIIGKEKTKITNLLKNYENAFNALNDAS
ELFDLANSENDIETLQALFNDAPKLEDTITSLEISMLLSGENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGF
KVETLDFQEGEEAGLKDVSFLVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELMKQQDSANSSEKSEIGWGHQI
RSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLIALKAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=258, Percent_Identity=36.4341085271318, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI34577120, Length=243, Percent_Identity=34.9794238683128, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI166795305, Length=172, Percent_Identity=38.3720930232558, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI2367172, Length=332, Percent_Identity=50, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787462, Length=305, Percent_Identity=37.7049180327869, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=320, Percent_Identity=28.125, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=31.5112540192926, Blast_Score=149, Evalue=8e-37,
Organism=Drosophila melanogaster, GI19921226, Length=238, Percent_Identity=38.2352941176471, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41122; Mature: 41122

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYEFSELLKTLKNKVGNIASIIKPKNIQTRLKEIEELENSPSFWNDVKQAGIIGKEKT
CCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
KITNLLKNYENAFNALNDASELFDLANSENDIETLQALFNDAPKLEDTITSLEISMLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHEEEEEEEEC
ENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGFKVETLDFQEGEEAGLKDVSF
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEE
LVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
EEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCEEEEEECCCEEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELM
EEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQDSANSSEKSEIGWGHQIRSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLI
HHHCCCCCCCHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHE
ALKAE
EEECC
>Mature Secondary Structure
MDNYEFSELLKTLKNKVGNIASIIKPKNIQTRLKEIEELENSPSFWNDVKQAGIIGKEKT
CCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
KITNLLKNYENAFNALNDASELFDLANSENDIETLQALFNDAPKLEDTITSLEISMLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHEEEEEEEEC
ENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGFKVETLDFQEGEEAGLKDVSF
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEE
LVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
EEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCEEEEEECCCEEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELM
EEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQDSANSSEKSEIGWGHQIRSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLI
HHHCCCCCCCHHHCCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHE
ALKAE
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA