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The map label for this gene is hisF-1 [H]

Identifier: 153952216

GI number: 153952216

Start: 355404

End: 356150

Strand: Direct

Name: hisF-1 [H]

Synonym: JJD26997_0401

Alternate gene names: 153952216

Gene position: 355404-356150 (Clockwise)

Preceding gene: 153952021

Following gene: 153951119

Centisome position: 19.26

GC content: 32.4

Gene sequence:

>747_bases
ATGTTAAAAACTAGAATTATCCCCTGTGTGTTATTAAAAAATTCTCAACTTGTTAAAAGTATAGAATTTAAAGATTTTCG
CACTATAGGGCACTTAACTTCAACGGTGCGTATTTACAATGCACGCAATGTTGATGAGCTTATTATTTTAGATATAGATG
CTTCAAAAAGCGGAGAAATAGACTTTGAAAGCATAGAAGATCTTGCTAAAGAATGTTTTATGCCCCTAACTATAGGTGGT
GGGATAAAAACTCTTGAAGATATACAAAAGATTTTAAATTTAGGGGCGGATAAAATTTCTATCAATTCTAAAGCCTTAGA
AGATATGGATTTTATAAGTAAAGCGGCTAATCGTTTTGGCTCGCAATGTATAGTTTGTTCTATAGATGTTAAAAGAAAAG
GGGGTCAATTTTGTGTTTATGATAGGGGAAATTTACTTGAAAAAAGTCCTTTAGAACTTGCTTTAGAATATGAAAAAAAA
GGAGCTGGAGAGCTTCTTTTAACTTCTGTAGATTTTGAAGGAAAAGCAAGGGGTTATGATTTAGAACTTTTAAAGATTTT
TCAAAATAAACTTAAAATTCCTCTTATTATCAATGGAGGGCTTGGCAAGCCAAGTGATGGAGTTGAGGCTTTAAATTTGG
GTGCTGATGCTTTAGCGGGGGCTTATATTTTTCATTTTTCTAAATATACCCCTAAAGATGTAAAAGAAGAATTAGCTAAG
CAAGGCTTTGCAGTGAGATTGCTTTAA

Upstream 100 bases:

>100_bases
CGCATCTTTGCAAAAAGATCATATTTTTGCGACGCAGTTTCATCCTGAAAAAAGTCAAAATTTAGGCTTAAAACTTTTAG
AAAATTTTGTAAGGCTTTAA

Downstream 100 bases:

>100_bases
GCAAACATAGTAGAAATCTTTATCTTCATCATAAATAGTAAAATGATTTTGTTGATATAATTTTAAAGCTTTATGATTGC
TTTTAAAAACATAAGCTTTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF2; ImGP synthase subunit hisF2; IGPS subunit hisF2 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLKTRIIPCVLLKNSQLVKSIEFKDFRTIGHLTSTVRIYNARNVDELIILDIDASKSGEIDFESIEDLAKECFMPLTIGG
GIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFGSQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKK
GAGELLLTSVDFEGKARGYDLELLKIFQNKLKIPLIINGGLGKPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAK
QGFAVRLL

Sequences:

>Translated_248_residues
MLKTRIIPCVLLKNSQLVKSIEFKDFRTIGHLTSTVRIYNARNVDELIILDIDASKSGEIDFESIEDLAKECFMPLTIGG
GIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFGSQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKK
GAGELLLTSVDFEGKARGYDLELLKIFQNKLKIPLIINGGLGKPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAK
QGFAVRLL
>Mature_248_residues
MLKTRIIPCVLLKNSQLVKSIEFKDFRTIGHLTSTVRIYNARNVDELIILDIDASKSGEIDFESIEDLAKECFMPLTIGG
GIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFGSQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKK
GAGELLLTSVDFEGKARGYDLELLKIFQNKLKIPLIINGGLGKPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAK
QGFAVRLL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=36.9649805447471, Blast_Score=160, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319725, Length=279, Percent_Identity=31.1827956989247, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR020021
- InterPro:   IPR006062
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27392; Mature: 27392

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRIIPCVLLKNSQLVKSIEFKDFRTIGHLTSTVRIYNARNVDELIILDIDASKSGEI
CCCCCEEEEEEECCCHHHHHHCHHHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCCCCC
DFESIEDLAKECFMPLTIGGGIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFG
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCC
SQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKKGAGELLLTSVDFEGKARGYD
CEEEEEEEEEECCCCCEEEECCCCCCCCCCHHEEEEECCCCCCEEEEEEECCCCCCCCCC
LELLKIFQNKLKIPLIINGGLGKPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAK
HHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
QGFAVRLL
CCCEEEEC
>Mature Secondary Structure
MLKTRIIPCVLLKNSQLVKSIEFKDFRTIGHLTSTVRIYNARNVDELIILDIDASKSGEI
CCCCCEEEEEEECCCHHHHHHCHHHHHHHHHHHHEEEEEECCCCCEEEEEEECCCCCCCC
DFESIEDLAKECFMPLTIGGGIKTLEDIQKILNLGADKISINSKALEDMDFISKAANRFG
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCC
SQCIVCSIDVKRKGGQFCVYDRGNLLEKSPLELALEYEKKGAGELLLTSVDFEGKARGYD
CEEEEEEEEEECCCCCEEEECCCCCCCCCCHHEEEEECCCCCCEEEEEEECCCCCCCCCC
LELLKIFQNKLKIPLIINGGLGKPSDGVEALNLGADALAGAYIFHFSKYTPKDVKEELAK
HHHHHHHHHCCEEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHH
QGFAVRLL
CCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]