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The map label for this gene is aspA [H]

Identifier: 153952003

GI number: 153952003

Start: 98099

End: 99505

Strand: Direct

Name: aspA [H]

Synonym: JJD26997_0094

Alternate gene names: 153952003

Gene position: 98099-99505 (Clockwise)

Preceding gene: 153950972

Following gene: 153951706

Centisome position: 5.32

GC content: 33.19

Gene sequence:

>1407_bases
ATGGGAACAAGAAAAGAACATGATTTTATTGGGGAATTAGAAATCTCTGATGAGGTTTATTATGGAGTGCAAACTTTTAG
AGCTGTTGAAAATTTTGATATTTCTCATGATAGATTAAAAGATTTTCCTCGCTTTGTTAGAGCTTTGGCTAGAGTAAAAA
AAGCTGCAGCTATGGCAAATCATGAATTAGGTCTTTTGGATAAAAATATTCAAGATGCGATTATTAAAGCGTGCGATAAA
ATTTTAGAAGGGGGATATTATGATCAATTTGTAGTGGATATGATCCAAGGAGGTGCTGGAACAAGTACAAATATGAATGC
TAATGAAGTTATAGCAAACATAGGACTTGAACTTATGGGTCATAAAAAAGGTGAATACCAATATCTTCATCCAAATGATC
ATGTTAATTTAAGCCAAAGTACTAATGATGCTTATCCTACAGCTTTACACCTTGCCTTGCATGATTATTTAAGTGATTTG
GCAAAAGCTATGGAACATCTAAAAAAAGCTTATGAGAGAAAAGCTGAAGAATTTAAAAATGTTCTTAAAATGGGTAGAAC
CCAACTGCAAGATGCTGTACCTATGACTTTAGGTCGTGAATTTAAAACTTTTGCTGTAATGATAGGCGAAGATATACAAA
GAGTATTAGAAGCTAGAAAATTGATTTTAGAGATCAATTTGGGTGGAACTGCTATTGGAACGGGAATTAATTCTCATCCT
GATTATCCGAAGGTTGTAGAAAGAAAAATAAGAGAAGTGACAGGTTTTGAATACACTGTGGCTGAGGATTTGATCGAGGC
GACTCAAGATACGGGAGCTTATGTACAAATTTCAGGTGTTTTAAAACGTGTTGCAACAAAACTTTCTAAAGTATGTAATG
ACTTAAGACTTTTAAGTAGTGGTCCAAAATGTGGTCTTAATGAGATTAATCTTCCAAAAATGCAACCAGGTAGTTCTATC
ATGCCAGGTAAGGTAAATCCTGTTATTCCTGAAGTAGTTAATCAAGTTTGTTATTTTGTTATTGGAGCAGATGTAACTGT
AACTTTTGCTTGTGAGGGTGGACAATTACAACTTAATGTTTTTGAACCAGTTGCAGCGTATAGCTTATTTAATTCTATTG
TAATGCTAGAAAAAGCAATGTATACTTTAGCCGATAAATGTATAGATGGTATTACCGCAAATGAAAAAATTTGTTCAGAC
TTTGTCTATAATTCAGTGGGTATAGTAACAGCTTTAAATCCTTATATTGGCTATGAAAATTCAGCTTCTATAGCTAAAGA
GGCAATGAACACTGGAAAAAGAGTAGCTGATATTGCACTTGAAAGAGGGCTTTTAAGTAAAGAACAAATTGATGAAATTT
TAACTCCATCAAATATGCTAAATCCTCACATGAAAGCTAAAAAATAG

Upstream 100 bases:

>100_bases
ATTAACCAATTATTAACTAAAAATGCAATATCATTATTTTTATGATTTAAATCAAAAAATTTTTGATTTTAAATTAAAAT
ACTTTTATGAAAGGTTCAAA

Downstream 100 bases:

>100_bases
CATTTTAGGATTTAATATGGATATAATGATAATTTTGCAAGTAATCGTTCTTCTTGGGGCGATTTTTATTGGTATTCGTC
TAGGTGGTATTGCTATAGGT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MGTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMANHELGLLDKNIQDAIIKACDK
ILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMGHKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDL
AKAMEHLKKAYERKAEEFKNVLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSSGPKCGLNEINLPKMQPGSSI
MPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNVFEPVAAYSLFNSIVMLEKAMYTLADKCIDGITANEKICSD
FVYNSVGIVTALNPYIGYENSASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMKAKK

Sequences:

>Translated_468_residues
MGTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMANHELGLLDKNIQDAIIKACDK
ILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMGHKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDL
AKAMEHLKKAYERKAEEFKNVLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSSGPKCGLNEINLPKMQPGSSI
MPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNVFEPVAAYSLFNSIVMLEKAMYTLADKCIDGITANEKICSD
FVYNSVGIVTALNPYIGYENSASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMKAKK
>Mature_467_residues
GTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMANHELGLLDKNIQDAIIKACDKI
LEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMGHKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDLA
KAMEHLKKAYERKAEEFKNVLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHPD
YPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSSGPKCGLNEINLPKMQPGSSIM
PGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNVFEPVAAYSLFNSIVMLEKAMYTLADKCIDGITANEKICSDF
VYNSVGIVTALNPYIGYENSASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMKAKK

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40.4347826086956, Blast_Score=348, Evalue=4e-96,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=57.9399141630901, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1787896, Length=466, Percent_Identity=38.412017167382, Blast_Score=342, Evalue=4e-95,
Organism=Escherichia coli, GI1790398, Length=331, Percent_Identity=22.6586102719033, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=41.5217391304348, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=40.6727828746177, Blast_Score=271, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=41.7391304347826, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=40.997830802603, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=40.1727861771058, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=40.997830802603, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=40.6047516198704, Blast_Score=357, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24583245, Length=466, Percent_Identity=35.6223175965665, Blast_Score=304, Evalue=9e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51750; Mature: 51619

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMAN
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HELGLLDKNIQDAIIKACDKILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHC
HKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDLAKAMEHLKKAYERKAEEFKN
CCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
HHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECCCCCCCC
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPKCGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCEEEEEEECCCEEEEEE
FEPVAAYSLFNSIVMLEKAMYTLADKCIDGITANEKICSDFVYNSVGIVTALNPYIGYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHEECCCCCCCCC
SASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMKAKK
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCCC
>Mature Secondary Structure 
GTRKEHDFIGELEISDEVYYGVQTFRAVENFDISHDRLKDFPRFVRALARVKKAAAMAN
CCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
HELGLLDKNIQDAIIKACDKILEGGYYDQFVVDMIQGGAGTSTNMNANEVIANIGLELMG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHC
HKKGEYQYLHPNDHVNLSQSTNDAYPTALHLALHDYLSDLAKAMEHLKKAYERKAEEFKN
CCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKMGRTQLQDAVPMTLGREFKTFAVMIGEDIQRVLEARKLILEINLGGTAIGTGINSHP
HHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECCCCCCCC
DYPKVVERKIREVTGFEYTVAEDLIEATQDTGAYVQISGVLKRVATKLSKVCNDLRLLSS
CCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPKCGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCYFVIGADVTVTFACEGGQLQLNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCEEEEEEECCCEEEEEE
FEPVAAYSLFNSIVMLEKAMYTLADKCIDGITANEKICSDFVYNSVGIVTALNPYIGYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHEECCCCCCCCC
SASIAKEAMNTGKRVADIALERGLLSKEQIDEILTPSNMLNPHMKAKK
HHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]