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The map label for this gene is maeB [H]

Identifier: 153951838

GI number: 153951838

Start: 384276

End: 385511

Strand: Direct

Name: maeB [H]

Synonym: JJD26997_0435

Alternate gene names: 153951838

Gene position: 384276-385511 (Clockwise)

Preceding gene: 153951105

Following gene: 153950941

Centisome position: 20.83

GC content: 35.84

Gene sequence:

>1236_bases
ATGAATTTAAAAGAAGAAGCTTTAAAATACCATTTGGGTGGGAAAATTGATATTGTGCCAAGTAAGCCAATGGCTACAAG
TCATGATCTATCTTTGGCGTATAGCCCAGGAGTTGCAGAACCTTGCCTTGAAATCGCTAAAGATAATGAGCTTGCTTATA
CTTATACAAACAAGGCTAATTTAGTAGCTATAGTTAGTGATGGTTCTGCTGTTTTAGGGCTTGGAAATATAGGAGCACAG
GCTTCAAAACCTGTTATGGAAGGTAAGGCTTGTTTGTTTAAAAAATTTGCTAATGTCAATGCTTATGATATAGAAATTGA
TGTTCATAGTATAGAAGAAATTGTAAATTTTTGTAAGGCTTTGGCTCCAGCTGTGGGTGGGATAAATTTAGAAGATATAG
CTGCGCCAAAATGTTTTGAAATCGAAGCAGCTTTGCAAGATTTAGGAATCCCTGTAATGCACGATGATCAGCACGGAACG
GCTATTATTTCAACTGCAGGGCTTATGAATGCTATGGAAATAAGCGGTAAAAAATTTAAAGACATTAAGGTGGTTGTAAG
TGGCGCAGGTGCCGCAGGTATAGCAAGTGCTAAGATGTATAGAAATTTAGGGGTTGAAAATATTATTTTAGTAGATAGCA
AAGGAGTGATTTCTAAAGATAGAAATGATTTAACTTCTCGAAAACTTGAATTTGTTGTTGATTCTAAGGAAAAGACTTTA
AAAGAAGCTTTAAAAGGAGCTGATGTTTTCCTAGGACTTAGTGCGCCAAAAATTCTTGATGATGAAATGGTTTTAAGCAT
GGCAAAAGATCCTGTGATTTTTGCTTTAGCAAATCCTATCCCTGAAGTGATGCCTGAAGATGTGGCTAGGCTTAGAAAAG
ATGCGATTGTAGGAACAGGAAGAAGTGATTATCCAAATCAAATCAACAATGTTTTAGGTTTTCCTTTTATTTTCCGTGGG
GCTTTAGATGTGCGTGCAAGTAAAATCACTGAAAATATGAAAGTAGCGGCTGCTAAAGCATTAGCAGATCTTGCAAAACT
TCCTGTAAGTGATGCGGTTAAAAAGGCTTATAATTTAAATCATTTAGAATTTGGAAGAGATTATGTTATACCAAAACCTT
TTGATGAAAGAGTAAAAGCTGCAGTAAGTACAGCTGTAGCAGCAGCTGCGGTAAAAGATGGTGTGGCTAAAGTGAAAAAT
TTTGATGAGAAAGCTTATTTTGAAAGCTTAAAATGA

Upstream 100 bases:

>100_bases
AGTGCAGTAAGTCCTAGTATTTTTGAAGTATTGGAATTTTTAGGGGTTGATGAGTGCAAAAAAAGAATAGAAAATTTTTT
AAAAGTTAGAGGAAAATAAA

Downstream 100 bases:

>100_bases
AAAATGTTCATTGCATCAATCATCCATTGATCGAACATAAGTTAGGAATTTTAAGGGTTAAAGAGACAAAGCCCTTTCAA
TTTCGTATGTTAATCGATGA

Product: NADP-dependent malic enzyme, truncation

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIVSDGSAVLGLGNIGAQ
ASKPVMEGKACLFKKFANVNAYDIEIDVHSIEEIVNFCKALAPAVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGT
AIISTAGLMNAMEISGKKFKDIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTSRKLEFVVDSKEKTL
KEALKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTGRSDYPNQINNVLGFPFIFRG
ALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLNHLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKN
FDEKAYFESLK

Sequences:

>Translated_411_residues
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIVSDGSAVLGLGNIGAQ
ASKPVMEGKACLFKKFANVNAYDIEIDVHSIEEIVNFCKALAPAVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGT
AIISTAGLMNAMEISGKKFKDIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTSRKLEFVVDSKEKTL
KEALKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTGRSDYPNQINNVLGFPFIFRG
ALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLNHLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKN
FDEKAYFESLK
>Mature_411_residues
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKANLVAIVSDGSAVLGLGNIGAQ
ASKPVMEGKACLFKKFANVNAYDIEIDVHSIEEIVNFCKALAPAVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGT
AIISTAGLMNAMEISGKKFKDIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTSRKLEFVVDSKEKTL
KEALKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTGRSDYPNQINNVLGFPFIFRG
ALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLNHLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKN
FDEKAYFESLK

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=369, Percent_Identity=27.3712737127371, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1788806, Length=417, Percent_Identity=53.2374100719424, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6322823, Length=292, Percent_Identity=27.7397260273973, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24646388, Length=344, Percent_Identity=28.4883720930233, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24646386, Length=344, Percent_Identity=28.4883720930233, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78707242, Length=393, Percent_Identity=24.1730279898219, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707238, Length=393, Percent_Identity=24.1730279898219, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI78707240, Length=390, Percent_Identity=23.8461538461538, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI281362674, Length=400, Percent_Identity=24.75, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281362672, Length=399, Percent_Identity=24.3107769423559, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356279, Length=399, Percent_Identity=24.3107769423559, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 44005; Mature: 44005

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKAN
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCC
LVAIVSDGSAVLGLGNIGAQASKPVMEGKACLFKKFANVNAYDIEIDVHSIEEIVNFCKA
EEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHH
LAPAVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGTAIISTAGLMNAMEISGKKFK
HHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCC
DIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTSRKLEFVVDSKEKTL
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHEEEEECCHHHHH
KEALKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTG
HHHHCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHEEECC
RSDYPNQINNVLGFPFIFRGALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLN
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
HLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKNFDEKAYFESLK
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MNLKEEALKYHLGGKIDIVPSKPMATSHDLSLAYSPGVAEPCLEIAKDNELAYTYTNKAN
CCCHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECCCC
LVAIVSDGSAVLGLGNIGAQASKPVMEGKACLFKKFANVNAYDIEIDVHSIEEIVNFCKA
EEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHH
LAPAVGGINLEDIAAPKCFEIEAALQDLGIPVMHDDQHGTAIISTAGLMNAMEISGKKFK
HHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHCCCCCC
DIKVVVSGAGAAGIASAKMYRNLGVENIILVDSKGVISKDRNDLTSRKLEFVVDSKEKTL
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHEEEEECCHHHHH
KEALKGADVFLGLSAPKILDDEMVLSMAKDPVIFALANPIPEVMPEDVARLRKDAIVGTG
HHHHCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHEEECC
RSDYPNQINNVLGFPFIFRGALDVRASKITENMKVAAAKALADLAKLPVSDAVKKAYNLN
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
HLEFGRDYVIPKPFDERVKAAVSTAVAAAAVKDGVAKVKNFDEKAYFESLK
CEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]