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The map label for this gene is gltX

Identifier: 153951105

GI number: 153951105

Start: 382883

End: 384274

Strand: Direct

Name: gltX

Synonym: JJD26997_0434

Alternate gene names: 153951105

Gene position: 382883-384274 (Clockwise)

Preceding gene: 153951320

Following gene: 153951838

Centisome position: 20.75

GC content: 33.48

Gene sequence:

>1392_bases
ATGCACGAAAAACTTACAACGCGTTTTGCTCCAAGCCCAACAGGGTATTTGCATATAGGTGGACTTAGAACAGCTCTTTA
TAATTATTTGTATGCTAGAAAAAATGGTGGAAATTTTTTACTTCGTATTGAAGATACGGATTTAAAAAGAAATTCAAAAG
AAGCTACTAAAGCAATTATTGAAGCTTTTAAATGGTGTGGTTTAGAACATGATGGAGAAGTAACCTACCAATCAGAGCGT
TTTGATCTTTATAAGGAGTATGTTAAAAAACTTTTAGATGAAGGTAAGGCGTATTATTGTTATATGAGTAAAGAAGAATT
AGAAGAATTGCGTGCCAAGCAAGAAGCCGCTAAAGAGCGCCCAAGATATGATGGAAGATATAGAGAATTTACAGGCACGC
CACCTCAGGGTATAGAACCTGTTGTGCGTATTAAAGCGCCTCAAAGCGGTGAGATTGTTTTTAAAGATGGGGTTAAAGGT
GAGGTGAGATTTAAAGCAGAGGATATAATGGATGATTTTATCATTGCAAGAAGCGATGGAACCCCAACTTATAATTTTAC
TGTTGTTATTGACGATGCTTTAATGGGGGTAAGTGATGTAATTCGCGGGGATGATCACCTTTCAAACACTCCTAAACAAA
TTGTGCTTTATGAAGCATTGGGTTTTAAAATTCCTCAATTTTTTCATGTGGCTATGATACATGGAGAAGATGGTAAAAAG
CTTTCAAAAAGACATGGAGCAACCGATGTTATGGAATACAAAGAAATGGGAATTTTACCTCAAGCTTTGCTTAATTTTCT
TGTGCGTTTAGGTTGGAGTCATGGTGATGATGAGGTTTTTTCATTAGAAGATTTGAAAAAGCTTTTTGATCCTTATCATA
TTAATAAAAGCGCATCTTGTTATAATGCTAAAAAGCTTGAATGGCTTAATACTCATTATATTAAAACTTTGCCTTTTGAA
GAAATCAAGCGTCAACTTAAAGATCTTGGTTTTGATTTATCTGTTTATGAAAAGGCAGGATTTTTACTTGATTTGTTAAG
AGAGCGTGCAAAAACCTTGCATGATATCATCAATGGTGCAAAAAGCATAGTTAATGCACCGCAAAATTATGATGAAAATG
CAGTGCAAAAATTTGTTAACGAAAATAATTTAGAATTGCTCCAGGCATTTGCTAATACACTTAAAGATCCAAAAACAGGT
AAAGATTTTGAAGATTTTACAAATGATTTTTTAGAAAAAAAAGAAGCAAAGTTAAAAGATTTGGCTCAGCCTATACGTAT
AGCTTTAACAGGGAGTGCAGTAAGTCCTAGTATTTTTGAAGTATTGGAATTTTTAGGGGTTGATGAGTGCAAAAAAAGAA
TAGAAAATTTTTTAAAAGTTAGAGGAAAATAA

Upstream 100 bases:

>100_bases
TGCAAAAAATGCAAAACTTAATAAAATTTTTTTCATTTTAAACCTTTATTTAAGTATAAATACCGCATTATATCAATAAA
AATTTAAGAAAAGGCAAAAA

Downstream 100 bases:

>100_bases
AATGAATTTAAAAGAAGAAGCTTTAAAATACCATTTGGGTGGGAAAATTGATATTGTGCCAAGTAAGCCAATGGCTACAA
GTCATGATCTATCTTTGGCG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAIIEAFKWCGLEHDGEVTYQSER
FDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKERPRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKG
EVRFKAEDIMDDFIIARSDGTPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPQFFHVAMIHGEDGKK
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASCYNAKKLEWLNTHYIKTLPFE
EIKRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGAKSIVNAPQNYDENAVQKFVNENNLELLQAFANTLKDPKTG
KDFEDFTNDFLEKKEAKLKDLAQPIRIALTGSAVSPSIFEVLEFLGVDECKKRIENFLKVRGK

Sequences:

>Translated_463_residues
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAIIEAFKWCGLEHDGEVTYQSER
FDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKERPRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKG
EVRFKAEDIMDDFIIARSDGTPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPQFFHVAMIHGEDGKK
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASCYNAKKLEWLNTHYIKTLPFE
EIKRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGAKSIVNAPQNYDENAVQKFVNENNLELLQAFANTLKDPKTG
KDFEDFTNDFLEKKEAKLKDLAQPIRIALTGSAVSPSIFEVLEFLGVDECKKRIENFLKVRGK
>Mature_463_residues
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAIIEAFKWCGLEHDGEVTYQSER
FDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKERPRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKG
EVRFKAEDIMDDFIIARSDGTPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPQFFHVAMIHGEDGKK
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASCYNAKKLEWLNTHYIKTLPFE
EIKRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGAKSIVNAPQNYDENAVQKFVNENNLELLQAFANTLKDPKTG
KDFEDFTNDFLEKKEAKLKDLAQPIRIALTGSAVSPSIFEVLEFLGVDECKKRIENFLKVRGK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=487, Percent_Identity=29.9794661190965, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI62241042, Length=259, Percent_Identity=25.8687258687259, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4826960, Length=293, Percent_Identity=23.5494880546075, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788743, Length=462, Percent_Identity=43.7229437229437, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI226510925, Length=278, Percent_Identity=35.2517985611511, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1786895, Length=207, Percent_Identity=29.4685990338164, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=32.5865580448065, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17511007, Length=236, Percent_Identity=25.4237288135593, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17543332, Length=118, Percent_Identity=32.2033898305085, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=34.8979591836735, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321192, Length=245, Percent_Identity=27.7551020408163, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=307, Percent_Identity=27.0358306188925, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=31.9018404907975, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17864488, Length=230, Percent_Identity=27.3913043478261, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_CAMJD (A7H295)

Other databases:

- EMBL:   CP000768
- RefSeq:   YP_001397625.1
- STRING:   A7H295
- GeneID:   5389780
- GenomeReviews:   CP000768_GR
- KEGG:   cjd:JJD26997_0434
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   CJEJ360109:JJD26997_0434-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53086; Mature: 53086

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAII
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHHH
EAFKWCGLEHDGEVTYQSERFDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKER
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
PRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKGEVRFKAEDIMDDFIIARSDG
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEHHHHHHHHEEEECCC
TPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPQFFHVAMIHGEDGKK
CCCEEEEEEECCHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCCCCEEEEEEEECCCCHH
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASC
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECHHHHHHHHCCCCCCCCCCC
YNAKKLEWLNTHYIKTLPFEEIKRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGA
CCCHHHHHHCCHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSIVNAPQNYDENAVQKFVNENNLELLQAFANTLKDPKTGKDFEDFTNDFLEKKEAKLKD
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LAQPIRIALTGSAVSPSIFEVLEFLGVDECKKRIENFLKVRGK
HHHHHEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHEKLTTRFAPSPTGYLHIGGLRTALYNYLYARKNGGNFLLRIEDTDLKRNSKEATKAII
CCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHEECCCEEEEEEECCCCCCCHHHHHHHHH
EAFKWCGLEHDGEVTYQSERFDLYKEYVKKLLDEGKAYYCYMSKEELEELRAKQEAAKER
HHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHC
PRYDGRYREFTGTPPQGIEPVVRIKAPQSGEIVFKDGVKGEVRFKAEDIMDDFIIARSDG
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCEEEEEHHHHHHHHEEEECCC
TPTYNFTVVIDDALMGVSDVIRGDDHLSNTPKQIVLYEALGFKIPQFFHVAMIHGEDGKK
CCCEEEEEEECCHHHHHHHHHCCCCCCCCCCHHEEEEHHHCCCCCCEEEEEEEECCCCHH
LSKRHGATDVMEYKEMGILPQALLNFLVRLGWSHGDDEVFSLEDLKKLFDPYHINKSASC
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEECHHHHHHHHCCCCCCCCCCC
YNAKKLEWLNTHYIKTLPFEEIKRQLKDLGFDLSVYEKAGFLLDLLRERAKTLHDIINGA
CCCHHHHHHCCHHEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSIVNAPQNYDENAVQKFVNENNLELLQAFANTLKDPKTGKDFEDFTNDFLEKKEAKLKD
HHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LAQPIRIALTGSAVSPSIFEVLEFLGVDECKKRIENFLKVRGK
HHHHHEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA