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The map label for this gene is dapF [H]

Identifier: 153951653

GI number: 153951653

Start: 1662358

End: 1663107

Strand: Direct

Name: dapF [H]

Synonym: JJD26997_1883

Alternate gene names: 153951653

Gene position: 1662358-1663107 (Clockwise)

Preceding gene: 153951069

Following gene: 153951430

Centisome position: 90.1

GC content: 32.27

Gene sequence:

>750_bases
GTGAAATTTTATAAATATTGTGCAAGTGGAAATGATTTTGTCATAACAAATGCTGATAGAAAAGAAGATAGAAGTTCTTT
AGCTAAAGAACTTTGCAATCGTTATGAGGGCATAGGTGCGGATGGCTTTATCGTGATATTGCCTCATGAAAAATACGACT
TTGAATGGGAATTTTATAATAACGATGGCTCAAGGGCTGCTATGTGTGGCAATGGCTCAAGGGCTGCAGCACATTTTGCA
CATCATATCAACAAAATAAACCCTAGCATGATTTTTTTAACCGGTGCAGGAGTAATAAAAGCAAAGGTAAATCAAGATAA
AGTTGAAGTATCTTTAGGCAAAATAAAAAGCGTGCAAAATACTTTTGAAAAATTAGGAAAAACTTGGCAACTTTGCGACA
CTGGAGTTCCACATCTAGTACATTTTTGTCAAAGTTTAGATGAATTTGATACAATGCTTTGTCAAAAAATGAGACAAAAA
TATAATGCCAATGTTAATTTTGTAAAAATTTTAGATGAAAATCATTTAAAAGTTCGTACCTATGAACGCGGAGTTGAAGA
TGAAACCTTAGCTTGTGGCACAGGAATGGGGGCTTGTTTTTATCTAGCATTTTTAAATAAAAAAGTACAAAATAAAGTTA
AAATAACTCCTAAAAGTGGTGAAGAGATGGGATTTGCATATAAAAATGAAGAATTGTTTTTTGAGGGAAAGGTAAAGTAT
TGTTTTGAAGCCAATTATAATTTTTCTTAG

Upstream 100 bases:

>100_bases
AACTTAAAAAAGCAGATTTTATCATTAAAAATACTAATTCTTATGCCGATTTTAGGCAAGAATGTGTTAAAGTAATACAA
GAAATTTCTAAAGGAAAAAT

Downstream 100 bases:

>100_bases
TCTTTTTCTAATCCCACTTTTTGCTGCTGATGATCTAAAGAATGGATTTGGAGAGGAATATTATAAACTTGATATAGATC
AAAAAAGACAAATTTTTTTT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKFYKYCASGNDFVITNADRKEDRSSLAKELCNRYEGIGADGFIVILPHEKYDFEWEFYNNDGSRAAMCGNGSRAAAHFA
HHINKINPSMIFLTGAGVIKAKVNQDKVEVSLGKIKSVQNTFEKLGKTWQLCDTGVPHLVHFCQSLDEFDTMLCQKMRQK
YNANVNFVKILDENHLKVRTYERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEMGFAYKNEELFFEGKVKY
CFEANYNFS

Sequences:

>Translated_249_residues
MKFYKYCASGNDFVITNADRKEDRSSLAKELCNRYEGIGADGFIVILPHEKYDFEWEFYNNDGSRAAMCGNGSRAAAHFA
HHINKINPSMIFLTGAGVIKAKVNQDKVEVSLGKIKSVQNTFEKLGKTWQLCDTGVPHLVHFCQSLDEFDTMLCQKMRQK
YNANVNFVKILDENHLKVRTYERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEMGFAYKNEELFFEGKVKY
CFEANYNFS
>Mature_249_residues
MKFYKYCASGNDFVITNADRKEDRSSLAKELCNRYEGIGADGFIVILPHEKYDFEWEFYNNDGSRAAMCGNGSRAAAHFA
HHINKINPSMIFLTGAGVIKAKVNQDKVEVSLGKIKSVQNTFEKLGKTWQLCDTGVPHLVHFCQSLDEFDTMLCQKMRQK
YNANVNFVKILDENHLKVRTYERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEMGFAYKNEELFFEGKVKY
CFEANYNFS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=24.8175182481752, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 28357; Mature: 28357

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYKYCASGNDFVITNADRKEDRSSLAKELCNRYEGIGADGFIVILPHEKYDFEWEFYN
CCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEC
NDGSRAAMCGNGSRAAAHFAHHINKINPSMIFLTGAGVIKAKVNQDKVEVSLGKIKSVQN
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCEEEHHHHHHHHHH
TFEKLGKTWQLCDTGVPHLVHFCQSLDEFDTMLCQKMRQKYNANVNFVKILDENHLKVRT
HHHHHCCEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
YERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEMGFAYKNEELFFEGKVKY
ECCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCEEEEECCEEE
CFEANYNFS
EEECCCCCC
>Mature Secondary Structure
MKFYKYCASGNDFVITNADRKEDRSSLAKELCNRYEGIGADGFIVILPHEKYDFEWEFYN
CCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEC
NDGSRAAMCGNGSRAAAHFAHHINKINPSMIFLTGAGVIKAKVNQDKVEVSLGKIKSVQN
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCEEEHHHHHHHHHH
TFEKLGKTWQLCDTGVPHLVHFCQSLDEFDTMLCQKMRQKYNANVNFVKILDENHLKVRT
HHHHHCCEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
YERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEMGFAYKNEELFFEGKVKY
ECCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCEEEEECCEEE
CFEANYNFS
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA