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The map label for this gene is hydB [H]

Identifier: 153951533

GI number: 153951533

Start: 407043

End: 408758

Strand: Direct

Name: hydB [H]

Synonym: JJD26997_0459

Alternate gene names: 153951533

Gene position: 407043-408758 (Clockwise)

Preceding gene: 153951982

Following gene: 153952637

Centisome position: 22.06

GC content: 35.84

Gene sequence:

>1716_bases
ATGAGTCAAAGAATTATTGTAGATCCTATTACAAGAATTGAAGGACATTTAAGAGTTGAAGTTGTTGTTGATGATAACAA
TGTGGTAAAAGAAGCTTATGCGGGTTCTACTTTGTGGCGTGGTATTGAAACCATAGTAAAAGGGCGTGATCCAAGAGATG
CGGGTTTTATGACACAAAGAATTTGTGGGGTTTGTACTTTTTCACATTATAAAGCAGGTATAGTTGCTGTTGAAAATGCT
TTAGGTATTACTCCGCCTTTAAATGCGCTTTTAACAAGAACTTTAATGAATGCGACTTTATTTTTACACGATCATATTGT
GCATTTTTATCAACTTCATGGGCTTGATTGGGCTGATGTGGTAAGTGCTTTAAGTGCTGATGTTAAAAAAGCAAGTGATG
AAGCTTTTAAATACACTCCAAATCCTTATGCAACAGGTGCGGATAAACTTCTTGAAGTGCAACAAAGACTTAAAACTTTT
GTAGATAAAGGAAATTTAGGACCTTTTGCTAATGCGTACTATGGTCATCCAACTTATCGTTTAACTCCTGAGCAAAATTT
AATCGTTCTTTCTCACTATCTAGAATGCTTAAGAATTCAAAGAATCATTGCTCAATGTATGGCGATTTTTGGAGCTAAAA
ATCCACATCCACAAAGTTTAACTGTTGGTGGCGTAACTTGCGTTATGGATTTGCTTGATCCTGCAAGAATGGGTGAATAT
ATGGTTAAATTCCAAGAAGTGCAAGACTTTGTTAATCGTGCGTATTATCCTGATCTTGCGATGGCTGGTAAAGCTTATGC
GCATGAAGCAAGTGTTTTAAACGATATAGGGGTAAATAATCTTTACACCTTTAAAGAATTCCAAATTGGAAGAGATGAAT
GGCTTTTTGAAAGTGGTATTATCAAAAACGGAGATTTAAGCAAAGTTTATGAAGTAGAAGAGGATAAGATTACTGAGGAA
GCAACTCATTCTTGGTATGCAGACAATGAGCCTTTACATCCTTATGAGGGTAAAACAAATCCAAATTATACAGGACTTGT
AGATGGAGAAAGCATGGATCATCATGGAAATAATGTCCATTCTAAAGTGTTTGATACCAAGGGTAAATATAGCTGGATTA
AAGCTCCACGCTATGAAGGAAACCCTATGCAAGTAGGACCTTTAGCAAATATCGTTGTAAATTATGCTAAGGGTAATCCA
AATGTTGTTCCTGTAGTAGATGAGTTCTTAAAAGAAACAGGACTTCCTTTAAATGCTGTATTTAGCACACTTGGAAGAAC
AGCTGCTCGTTGTATTGAAGCAAAAATCGTTGCAAATAATGCTTTAAAAGCGTTTAATAATTTAGTTGAAAATTTAAAAG
TAGATCAGAGTACTTGTGCTCCTTATGTGATCGATAATTCTAAAGAATACAAAGGACGCTATATGGGGCATGTGCCACGC
GGAACATTAAGCCATTGGTGTAGAATTAAAAATGGCGTCATTGAAAACTGGCAAGCTGTGGTGCCTTCTACTTGGAATGC
AAGTCCAAAAGATGCAAATGGTGTAGGTGGAAGTTATGAGCAATGTTTGATAGGACTTAAAATCGCTGATGTAAAACAAC
CGCTTGAAATCATTAGAAAAATTCACTCTTATGATCCTTGTATCGCGTGTGCGGTGCATGTAATGGATACTAAGGGTAAT
AATCTAAGCGAATATAAAGTGAATGTCAATTTATAA

Upstream 100 bases:

>100_bases
GTGTTTCAGATAAAATCGGTATAGGTGTGCTCACACTCACAGGTGTTGCTATAGCAGCACACGCAGTTATATCTTCCATG
CAAAAAGATAAGGAATAAAA

Downstream 100 bases:

>100_bases
GGAGGCTTTCATGCAAAATAAAGAAGAAAAATTGCAAAGAAAAGCTGAATATGAATTTAGCATAGGGTTACGTTTAACAC
ACTGGGTAAGAGCTGTGGCT

Product: quinone-reactive Ni/Fe-hydrogenase, large subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSQRIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQRICGVCTFSHYKAGIVAVENA
LGITPPLNALLTRTLMNATLFLHDHIVHFYQLHGLDWADVVSALSADVKKASDEAFKYTPNPYATGADKLLEVQQRLKTF
VDKGNLGPFANAYYGHPTYRLTPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
MVKFQEVQDFVNRAYYPDLAMAGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGIIKNGDLSKVYEVEEDKITEE
ATHSWYADNEPLHPYEGKTNPNYTGLVDGESMDHHGNNVHSKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNP
NVVPVVDEFLKETGLPLNAVFSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRKIHSYDPCIACAVHVMDTKGN
NLSEYKVNVNL

Sequences:

>Translated_571_residues
MSQRIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQRICGVCTFSHYKAGIVAVENA
LGITPPLNALLTRTLMNATLFLHDHIVHFYQLHGLDWADVVSALSADVKKASDEAFKYTPNPYATGADKLLEVQQRLKTF
VDKGNLGPFANAYYGHPTYRLTPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
MVKFQEVQDFVNRAYYPDLAMAGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGIIKNGDLSKVYEVEEDKITEE
ATHSWYADNEPLHPYEGKTNPNYTGLVDGESMDHHGNNVHSKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNP
NVVPVVDEFLKETGLPLNAVFSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRKIHSYDPCIACAVHVMDTKGN
NLSEYKVNVNL
>Mature_570_residues
SQRIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQRICGVCTFSHYKAGIVAVENAL
GITPPLNALLTRTLMNATLFLHDHIVHFYQLHGLDWADVVSALSADVKKASDEAFKYTPNPYATGADKLLEVQQRLKTFV
DKGNLGPFANAYYGHPTYRLTPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEYM
VKFQEVQDFVNRAYYPDLAMAGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGIIKNGDLSKVYEVEEDKITEEA
THSWYADNEPLHPYEGKTNPNYTGLVDGESMDHHGNNVHSKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNPN
VVPVVDEFLKETGLPLNAVFSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPRG
TLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRKIHSYDPCIACAVHVMDTKGNN
LSEYKVNVNL

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=588, Percent_Identity=40.8163265306122, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789368, Length=591, Percent_Identity=41.6243654822335, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 63654; Mature: 63523

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQR
CCCCEEECCHHHCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
ICGVCTFSHYKAGIVAVENALGITPPLNALLTRTLMNATLFLHDHIVHFYQLHGLDWADV
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VSALSADVKKASDEAFKYTPNPYATGADKLLEVQQRLKTFVDKGNLGPFANAYYGHPTYR
HHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LTPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCHHHHHHH
MVKFQEVQDFVNRAYYPDLAMAGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGI
HHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHCCC
IKNGDLSKVYEVEEDKITEEATHSWYADNEPLHPYEGKTNPNYTGLVDGESMDHHGNNVH
CCCCCCHHHEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
SKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNPNVVPVVDEFLKETGLPLNAV
HHHHCCCCCEEEEECCCCCCCCEEECCHHHHHEEECCCCCCEEHHHHHHHHHCCCCHHHH
FSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCHHHCCCCCCCCCC
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRK
HHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCHHHHEEECCHHHHHHHHHHHHH
IHSYDPCIACAVHVMDTKGNNLSEYKVNVNL
HHCCCCHHHHHEEEEECCCCCCEEEEEEECC
>Mature Secondary Structure 
SQRIIVDPITRIEGHLRVEVVVDDNNVVKEAYAGSTLWRGIETIVKGRDPRDAGFMTQR
CCCEEECCHHHCCCEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHH
ICGVCTFSHYKAGIVAVENALGITPPLNALLTRTLMNATLFLHDHIVHFYQLHGLDWADV
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VSALSADVKKASDEAFKYTPNPYATGADKLLEVQQRLKTFVDKGNLGPFANAYYGHPTYR
HHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LTPEQNLIVLSHYLECLRIQRIIAQCMAIFGAKNPHPQSLTVGGVTCVMDLLDPARMGEY
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHCHHHHHHH
MVKFQEVQDFVNRAYYPDLAMAGKAYAHEASVLNDIGVNNLYTFKEFQIGRDEWLFESGI
HHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCCEEEEHHHCCCCHHHHHHCCC
IKNGDLSKVYEVEEDKITEEATHSWYADNEPLHPYEGKTNPNYTGLVDGESMDHHGNNVH
CCCCCCHHHEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCH
SKVFDTKGKYSWIKAPRYEGNPMQVGPLANIVVNYAKGNPNVVPVVDEFLKETGLPLNAV
HHHHCCCCCEEEEECCCCCCCCEEECCHHHHHEEECCCCCCEEHHHHHHHHHCCCCHHHH
FSTLGRTAARCIEAKIVANNALKAFNNLVENLKVDQSTCAPYVIDNSKEYKGRYMGHVPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCCHHHCCCCCCCCCC
GTLSHWCRIKNGVIENWQAVVPSTWNASPKDANGVGGSYEQCLIGLKIADVKQPLEIIRK
HHHHHHHHHHCCHHHCCCEECCCCCCCCCCCCCCCCCCHHHHEEECCHHHHHHHHHHHHH
IHSYDPCIACAVHVMDTKGNNLSEYKVNVNL
HHCCCCHHHHHEEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]