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The map label for this gene is hydA [H]

Identifier: 153951982

GI number: 153951982

Start: 405901

End: 407040

Strand: Direct

Name: hydA [H]

Synonym: JJD26997_0458

Alternate gene names: 153951982

Gene position: 405901-407040 (Clockwise)

Preceding gene: 153951661

Following gene: 153951533

Centisome position: 22.0

GC content: 39.21

Gene sequence:

>1140_bases
ATGATTGATTATCATCAAATAGAGTCGCGTTTATCGGCTCTTGAAAAGCTTCCTTCTTTAAAAGATAATGATAGCATTGC
AAAAGCATTAGAAAAGTCAGGTTTTTCTAGAAGGGATTTTATGAAATGGGCGGGTGCTATGACGGCATTTTTGGCTTTGC
CAGCGAGTTTTACTCCTATGGTGGCAAAAGCTGCTGAGCTTGCTGATAGGCTTCCTGTTGTGTGGCTTCATATGGCTGAG
TGTACAGGCTGTAGCGAGAGTTTGTTAAGAAGTGATACTCCAACGATTGATAGTTTGATTTTTGATTATATTTCTTTAGA
ATATCACGAAACAGTGATGGCTGCAGCAGGTTGGCAAGCTGAAGAAAATTTAGAAAGTGCCATTCAAAAACATAAAAATA
AATACATTTTAATGGTTGAAGGTGGTATCCCTATGGGTGATACAGAGCATTTTTTAACCATAGGTGCACATGGAAAAACA
GGCTATGAGCTTTCAAAAATGGCAAGTGAAAATGCTTTGGCTATTTTTGCTATAGGAACCTGTTCTAGCTTTGGAGGAAT
CCAAGCAGCAAGACCAAATCCTAGCAATGCACAGCCTTTAAGCAAAGTAACAAGCAAAACTGTGATCAATGTTCCAGGCT
GTCCTCCAAGTGAAAAAAATATAGTGGGAAATGTGCTCCATTATTTATTATTTGGTGAGTTACCAGCACTTGATGTGTAT
AATAGACCAAAATGGGCTTATGGTTTAAGAATTCATGATCTTTGTGAAAGACGCGGACATTTTGATGCGGGTGAGTTTGT
GCATGCCTTTGGAGATGAAGGAGCAAAACAAGGTTTTTGTCTTTATAAAGTAGGCTGTAAAGGGCCTTATACTTTCAATA
ACTGCTCAAGAGAGAGATTTAACCAGCACACTTCTTGGCCTATTCAAGCAGGGCATGGTTGCATAGGTTGTTCTGAACCT
GATTTTTGGGATACTATGGGACCTTTTGAAGAGCCTATGGCTAGTCGTAAATTTGATACCGTTTTTGGTTTGGGTGCAGA
TAGTGTTTCAGATAAAATCGGTATAGGTGTGCTCACACTCACAGGTGTTGCTATAGCAGCACACGCAGTTATATCTTCCA
TGCAAAAAGATAAGGAATAA

Upstream 100 bases:

>100_bases
GATACTAATATTTGTTTATGAGAGTAAGTTTTTAACTTTATTTTAACTTCCTTTATTTTATTATTATAGAGAATAAATTT
CATGTTATAAAGGAAATATA

Downstream 100 bases:

>100_bases
AAATGAGTCAAAGAATTATTGTAGATCCTATTACAAGAATTGAAGGACATTTAAGAGTTGAAGTTGTTGTTGATGATAAC
AATGTGGTAAAAGAAGCTTA

Product: quinone-reactive Ni/Fe-hydrogenase, small subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIDYHQIESRLSALEKLPSLKDNDSIAKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMAE
CTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMVEGGIPMGDTEHFLTIGAHGKT
GYELSKMASENALAIFAIGTCSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVY
NRPKWAYGLRIHDLCERRGHFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSEP
DFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE

Sequences:

>Translated_379_residues
MIDYHQIESRLSALEKLPSLKDNDSIAKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMAE
CTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMVEGGIPMGDTEHFLTIGAHGKT
GYELSKMASENALAIFAIGTCSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVY
NRPKWAYGLRIHDLCERRGHFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSEP
DFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE
>Mature_379_residues
MIDYHQIESRLSALEKLPSLKDNDSIAKALEKSGFSRRDFMKWAGAMTAFLALPASFTPMVAKAAELADRLPVVWLHMAE
CTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQAEENLESAIQKHKNKYILMVEGGIPMGDTEHFLTIGAHGKT
GYELSKMASENALAIFAIGTCSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVY
NRPKWAYGLRIHDLCERRGHFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERFNQHTSWPIQAGHGCIGCSEP
DFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTLTGVAIAAHAVISSMQKDKE

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=340, Percent_Identity=45, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1789371, Length=292, Percent_Identity=45.8904109589041, Blast_Score=259, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 41410; Mature: 41410

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00024 HEMOPEXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYHQIESRLSALEKLPSLKDNDSIAKALEKSGFSRRDFMKWAGAMTAFLALPASFTPM
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHH
VAKAAELADRLPVVWLHMAECTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQA
HHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
EENLESAIQKHKNKYILMVEGGIPMGDTEHFLTIGAHGKTGYELSKMASENALAIFAIGT
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEEEEC
CSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVY
CCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
NRPKWAYGLRIHDLCERRGHFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERF
CCCCCEECCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
NQHTSWPIQAGHGCIGCSEPDFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHEECCCCHHCHHHCCHHHHH
TGVAIAAHAVISSMQKDKE
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDYHQIESRLSALEKLPSLKDNDSIAKALEKSGFSRRDFMKWAGAMTAFLALPASFTPM
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHH
VAKAAELADRLPVVWLHMAECTGCSESLLRSDTPTIDSLIFDYISLEYHETVMAAAGWQA
HHHHHHHHHCCCEEEEEHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
EENLESAIQKHKNKYILMVEGGIPMGDTEHFLTIGAHGKTGYELSKMASENALAIFAIGT
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCEEEEEEEC
CSSFGGIQAARPNPSNAQPLSKVTSKTVINVPGCPPSEKNIVGNVLHYLLFGELPALDVY
CCCCCCCCCCCCCCCCCCHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
NRPKWAYGLRIHDLCERRGHFDAGEFVHAFGDEGAKQGFCLYKVGCKGPYTFNNCSRERF
CCCCCEECCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
NQHTSWPIQAGHGCIGCSEPDFWDTMGPFEEPMASRKFDTVFGLGADSVSDKIGIGVLTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHEECCCCHHCHHHCCHHHHH
TGVAIAAHAVISSMQKDKE
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]