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The map label for this gene is lepA [H]

Identifier: 153951314

GI number: 153951314

Start: 53802

End: 54632

Strand: Direct

Name: lepA [H]

Synonym: JJD26997_0046

Alternate gene names: 153951314

Gene position: 53802-54632 (Clockwise)

Preceding gene: 153951784

Following gene: 153952213

Centisome position: 2.92

GC content: 32.49

Gene sequence:

>831_bases
ATGAAAGAAAATATTTTAAATGACATCAAGGGCTTTGCTAATGCGACACTTGAACGATTAAAAAGTTTTGATAAAGATAT
TGTAAAAGACTTAACTCCAGATATAACTTCAAAAGAGTTAAGCTCTTTATTTAGCTATGAATTTAAAAGCGACAATGGAG
TATCATTTAATAAAAGAGGCAGTGGTGTTAAAAGGTTATTTTTATTAAGTTTCTTTTTGGAAGATGCCGAAAGAAAGCAT
CAATCTAATATGATTTATGCAATTGAAGAACCAGAAACATCGCAACATCCAAACTATCAAAGAATAATTATTGAGTCTTT
AAAAAAATTAGCACAGAACAAAGGGCGTCAAATATTGCTTACAACACATACTCCTGAGATTGCAAGAATGGTTAATAAGG
AAAATATTATTTTTATACAAAAAGATGACAAAAATAAACGAATAGTTTATCATGGAGATGAAATTGATATAGAGATTCTT
GTCGATGCTTTAAGCATTATTGTGCCAAATTCTAAGGCTTTGAGTAAGGGTAGGGAACTTGTCAAAGCGATGAAGGAGAT
AGTGCCAAGACAGCTTTTTGAAGTGGCGATTCAAGCTAGTATAGGCAATAAAATCATAGCGCGTGAAAATGTCAAATCTA
TGGGCAAAAATGTAACGGCTAAATGTTATGGCGGGGATATAACGCGTAAAAGAAAGCTTTTAGAAAAGCAAAAAGAAGGT
AAAAAGCGTATGAAAGCCATAGGTAAGGTGCATTTACCGCAAGAAGCCTTTTTAAGTGTGCTTAAGATCGATATGCTTAA
AGCCAAAAGAAATATGCCATTAAAGCAATGA

Upstream 100 bases:

>100_bases
AGCTGATAGAACAAATACAGATAAAGATAACGAAATTACCCAAATCACAAAAGCTATCACACAAAATGCTATCTCTGAAA
TTGAACAAAAATTTAATGAT

Downstream 100 bases:

>100_bases
TGATAGAACAAAGAACATTAAGGTAAATTCCTACACGCACCATTTGAGACTGCTTAATGTAGCCCGTGCCAAAGACTATA
GCGTTTGGCGGAGTTGCGAC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKENILNDIKGFANATLERLKSFDKDIVKDLTPDITSKELSSLFSYEFKSDNGVSFNKRGSGVKRLFLLSFFLEDAERKH
QSNMIYAIEEPETSQHPNYQRIIIESLKKLAQNKGRQILLTTHTPEIARMVNKENIIFIQKDDKNKRIVYHGDEIDIEIL
VDALSIIVPNSKALSKGRELVKAMKEIVPRQLFEVAIQASIGNKIIARENVKSMGKNVTAKCYGGDITRKRKLLEKQKEG
KKRMKAIGKVHLPQEAFLSVLKIDMLKAKRNMPLKQ

Sequences:

>Translated_276_residues
MKENILNDIKGFANATLERLKSFDKDIVKDLTPDITSKELSSLFSYEFKSDNGVSFNKRGSGVKRLFLLSFFLEDAERKH
QSNMIYAIEEPETSQHPNYQRIIIESLKKLAQNKGRQILLTTHTPEIARMVNKENIIFIQKDDKNKRIVYHGDEIDIEIL
VDALSIIVPNSKALSKGRELVKAMKEIVPRQLFEVAIQASIGNKIIARENVKSMGKNVTAKCYGGDITRKRKLLEKQKEG
KKRMKAIGKVHLPQEAFLSVLKIDMLKAKRNMPLKQ
>Mature_276_residues
MKENILNDIKGFANATLERLKSFDKDIVKDLTPDITSKELSSLFSYEFKSDNGVSFNKRGSGVKRLFLLSFFLEDAERKH
QSNMIYAIEEPETSQHPNYQRIIIESLKKLAQNKGRQILLTTHTPEIARMVNKENIIFIQKDDKNKRIVYHGDEIDIEIL
VDALSIIVPNSKALSKGRELVKAMKEIVPRQLFEVAIQASIGNKIIARENVKSMGKNVTAKCYGGDITRKRKLLEKQKEG
KKRMKAIGKVHLPQEAFLSVLKIDMLKAKRNMPLKQ

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=102, Percent_Identity=57.843137254902, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1788922, Length=103, Percent_Identity=63.1067961165049, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=108, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=126, Percent_Identity=44.4444444444444, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI78706572, Length=102, Percent_Identity=47.0588235294118, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 31566; Mature: 31566

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENILNDIKGFANATLERLKSFDKDIVKDLTPDITSKELSSLFSYEFKSDNGVSFNKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
SGVKRLFLLSFFLEDAERKHQSNMIYAIEEPETSQHPNYQRIIIESLKKLAQNKGRQILL
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
TTHTPEIARMVNKENIIFIQKDDKNKRIVYHGDEIDIEILVDALSIIVPNSKALSKGREL
EECCHHHHHHHCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHH
VKAMKEIVPRQLFEVAIQASIGNKIIARENVKSMGKNVTAKCYGGDITRKRKLLEKQKEG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
KKRMKAIGKVHLPQEAFLSVLKIDMLKAKRNMPLKQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKENILNDIKGFANATLERLKSFDKDIVKDLTPDITSKELSSLFSYEFKSDNGVSFNKRG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
SGVKRLFLLSFFLEDAERKHQSNMIYAIEEPETSQHPNYQRIIIESLKKLAQNKGRQILL
CHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
TTHTPEIARMVNKENIIFIQKDDKNKRIVYHGDEIDIEILVDALSIIVPNSKALSKGREL
EECCHHHHHHHCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHH
VKAMKEIVPRQLFEVAIQASIGNKIIARENVKSMGKNVTAKCYGGDITRKRKLLEKQKEG
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHH
KKRMKAIGKVHLPQEAFLSVLKIDMLKAKRNMPLKQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA