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The map label for this gene is tyrS [H]

Identifier: 153951241

GI number: 153951241

Start: 399671

End: 400876

Strand: Direct

Name: tyrS [H]

Synonym: JJD26997_0454

Alternate gene names: 153951241

Gene position: 399671-400876 (Clockwise)

Preceding gene: 153952206

Following gene: 153951979

Centisome position: 21.66

GC content: 32.84

Gene sequence:

>1206_bases
ATGGATATAAAAAAAATATTAGCAGAAGTTAAGCGTGGCTGTGCAGAGCTTATTGATGAAGAAAGAATAGAAAAATTAAT
TAAAAATTATTATGAAAAGGGTGAAAATTTCTTTATAAAGGCAGGATTTGATCCTACAGCACCTGATTTACATTTAGGCC
ATAGCGTGGTTTTAACAAAAATGGCTTTTTTGCAAAAACATGGAGCTATTGTGCAGTTTTTGATTGGAGATTTTACGGGA
CAAATTGGCGATCCAAGTGGAAAAAGTGCAACGCGTAAAAAACTAGATAAAGAGCAAGTTTTAATCAATGCTAAAACTTA
TGAAACACAGGTTTTTAAGGTTTTGGATAAAGAAAAAACACAGATTAAGTTTAATTCTACTTGGCTTAATGAACTTGGAG
CCGCGGGTATAGTAGAGCTTGCTTCAACTTTTAGTGTAGCTAGAATGCTTGAGCGTGATGATTTTACCAAGAGATTTAAA
GAGCAAAGCCCTATTTCGATTTGCGAGTTTTTATATCCTTTGCTTCAAGGATATGATAGCGTGGCTTTAAAAAGCGATAT
TGAAATGGGTGGAACGGATCAAAAATTTAATCTTTTAATGGGAAGACAGCTTCAAAGAATTTATAGCATAGGAAAAGAAC
AAGCTGTGATTATGATGCCTTTACTTGAAGGTTTAGATGGTGTAAACAAAATGAGTAAGACTTTAAATAATTACATTGGT
GTTACAGAAAAAGCTAATGATATGTATGCTAAAATTTTAAGCATTAGCGATGAATTGATGTTTAGATACTATGAACTTTT
AAGTCAAAAAAGCCTTGAAGAAATCGCACAAATAAAAAAAGATATAGAGCAGGGCAATTTACATCCTAAAAAAGCTAAAG
AAAATTTAGCTTTAGAGATTACGGAGCGTTTTCATTCCAAAGAAGAGGCTAATAATGCTAAGTCTGAATTTGATAGAATT
CATTCTCAAAATGCTTTACCAAGTGATATGGCTGAGTTTGAAATACAAGGAAAAATTTGGCTTGCTAAGGCTTTGGTTGA
ATGTGGTTTGGAAAGTTCTACTTCTGCGGCAAGAAGAAGCATCAGCGCTAATGCAGTAAGTGTAAATTCTCAAAAAGTTA
GCGACGAGCAAATGCATTTAGAACAAGGAGAATACATCTTACAAATAGGTAAAAGAAAATTTGCAAAATTAAAGGTAAAA
GAATGA

Upstream 100 bases:

>100_bases
TCAATCCAGATAGTGTCAAGGATAGGTTAAAATCTCGTTGCAAGATTTTAGATTTTACATCTTTAAATGATGCTTATAAA
GAAGGTTAAAAAGGTTTATT

Downstream 100 bases:

>100_bases
ATTTACAACCCTTACAAATAGGAAAACATACTATAAAATATCCCATTTTTCAAGGGGGAATGGGACTAGGCATAAGCTGG
GATAGATTAGCTTCTGCGGT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTKMAFLQKHGAIVQFLIGDFTG
QIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKTQIKFNSTWLNELGAAGIVELASTFSVARMLERDDFTKRFK
EQSPISICEFLYPLLQGYDSVALKSDIEMGGTDQKFNLLMGRQLQRIYSIGKEQAVIMMPLLEGLDGVNKMSKTLNNYIG
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQGNLHPKKAKENLALEITERFHSKEEANNAKSEFDRI
HSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRSISANAVSVNSQKVSDEQMHLEQGEYILQIGKRKFAKLKVK
E

Sequences:

>Translated_401_residues
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTKMAFLQKHGAIVQFLIGDFTG
QIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKTQIKFNSTWLNELGAAGIVELASTFSVARMLERDDFTKRFK
EQSPISICEFLYPLLQGYDSVALKSDIEMGGTDQKFNLLMGRQLQRIYSIGKEQAVIMMPLLEGLDGVNKMSKTLNNYIG
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQGNLHPKKAKENLALEITERFHSKEEANNAKSEFDRI
HSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRSISANAVSVNSQKVSDEQMHLEQGEYILQIGKRKFAKLKVK
E
>Mature_401_residues
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTKMAFLQKHGAIVQFLIGDFTG
QIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKTQIKFNSTWLNELGAAGIVELASTFSVARMLERDDFTKRFK
EQSPISICEFLYPLLQGYDSVALKSDIEMGGTDQKFNLLMGRQLQRIYSIGKEQAVIMMPLLEGLDGVNKMSKTLNNYIG
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQGNLHPKKAKENLALEITERFHSKEEANNAKSEFDRI
HSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRSISANAVSVNSQKVSDEQMHLEQGEYILQIGKRKFAKLKVK
E

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=431, Percent_Identity=26.6821345707657, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1787925, Length=393, Percent_Identity=29.0076335877863, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=291, Percent_Identity=26.8041237113402, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=439, Percent_Identity=25.2847380410023, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20130383, Length=442, Percent_Identity=25.1131221719457, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45381; Mature: 45381

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
MAFLQKHGAIVQFLIGDFTGQIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKT
HHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHEEEECHHHHHHHHHHHHCHHH
QIKFNSTWLNELGAAGIVELASTFSVARMLERDDFTKRFKEQSPISICEFLYPLLQGYDS
EEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
VALKSDIEMGGTDQKFNLLMGRQLQRIYSIGKEQAVIMMPLLEGLDGVNKMSKTLNNYIG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCHHHHHHHHHHHHCC
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQGNLHPKKAKENLALEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
TERFHSKEEANNAKSEFDRIHSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCHHHHHHHHHHHCCCCCHHHHHHH
ISANAVSVNSQKVSDEQMHLEQGEYILQIGKRKFAKLKVKE
CCCCEEECCCHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDIKKILAEVKRGCAELIDEERIEKLIKNYYEKGENFFIKAGFDPTAPDLHLGHSVVLTK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHH
MAFLQKHGAIVQFLIGDFTGQIGDPSGKSATRKKLDKEQVLINAKTYETQVFKVLDKEKT
HHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHEEEECHHHHHHHHHHHHCHHH
QIKFNSTWLNELGAAGIVELASTFSVARMLERDDFTKRFKEQSPISICEFLYPLLQGYDS
EEEECHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
VALKSDIEMGGTDQKFNLLMGRQLQRIYSIGKEQAVIMMPLLEGLDGVNKMSKTLNNYIG
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCHHHHHHHHHHHHCC
VTEKANDMYAKILSISDELMFRYYELLSQKSLEEIAQIKKDIEQGNLHPKKAKENLALEI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
TERFHSKEEANNAKSEFDRIHSQNALPSDMAEFEIQGKIWLAKALVECGLESSTSAARRS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCHHHHHHHHHHHCCCCCHHHHHHH
ISANAVSVNSQKVSDEQMHLEQGEYILQIGKRKFAKLKVKE
CCCCEEECCCHHCCHHHHHHHCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]