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The map label for this gene is spoT [H]

Identifier: 153952206

GI number: 153952206

Start: 397488

End: 399659

Strand: Direct

Name: spoT [H]

Synonym: JJD26997_0453

Alternate gene names: 153952206

Gene position: 397488-399659 (Clockwise)

Preceding gene: 153951415

Following gene: 153951241

Centisome position: 21.54

GC content: 30.48

Gene sequence:

>2172_bases
TTGCTTGAACAATTAATCGATAATGTAAAAAATTGTAAAGATTTAGAAGCTGCTAAGGCTTTACTTTTCGAAATTTGTGG
CCATGATGCCATTCTTGAAAAAGCGGTAGATTACTGTATATTTTGTCATGAAGGGCAGTTTAGAAAAAGTGGAGAGCCTT
ATGCAGTACATCCTATTTTAGTAGCAACACTTGTTGGATTTTTAAGTGAAAATAAAAGCAATATTTTAGCTGCTTTATTG
CATGATGTGATCGAAGATACAAACTGCACCGAAGAAGAGCTTAGAGAGCAGTTTGGTTCTGAGGTTTTAAAACTTGTTTT
AGGACTTACTAAAATTATAGAAATTAGAGAAGATAATCTCATTTCATCAAAATCTAAAAAAAGTTTAACTAAATCAGCCT
TGACTTTCAGAAATATGCTTTTAGCGAGCATTGAAGATGTTGGGGTTTTGATTGTGAAGTTATGCGATAGGCTGCATAAT
ATGCTTACTCTTGATATTCTAAGAGAAGATAAGCAAAAAAGAATCAGCGAAGAAACTTTAGTTGTATATGCTCCAATAGC
TCATAGACTTGGTATTTCAAGCATAAAAAATTATTTAGAAGATCTTAGTTTTAAATATCTTATGCCAGATGAGTATAAGC
TTATTGACAATTACATCAATTCCAATAATCAAGAAATGCAATTAGGTTTAAATGAATTTATTTCCAAAATCGAACTTTTA
TTTTTAAGCAATGGCTTTAGACAAGGTAGTTTTGAAATTCAAAAACGCATTAAACATAGTTATTCTATTTATCTTAAAAT
GCAAAGAAAAGGTATTGGTATTGAAGAAGTTCTTGATTTGCTTGGGGTAAGAATTTTGGTTGAAAAAGTAAGTGATTGTT
ACTTAGCTTTAGGAATTTTACATACACATTTTAATCCCTTGGTTTCGCGTTTTAAGGATTATATAGCCTTGCCTAAACAA
AATGGTTATCAAACCATACATACCACACTTTTTGATGCTAAAAGTATTATAGAAGCACAAATTCGAACTTTTGATATGCA
TAAAATTGCGGAATTTGGTATAGCAGCACATTGGAAATACAAAGAAGATGGCAGTGTGGTTGCGCCTCGTCTTGATTGGC
TTACAGATATTTCAATGCAAAGTATAAACAATCTTGAAAATGCAGAAGATTATAATGCTATTGAACTTTATGAATATGCT
AAAGATAGTCTTTATGTTGAAGATGTCGCAGTTTATTCTCCTAAGGGAGAAATTTTCACTTTGCCTCGCGGTGCAACGGT
TCTTGATTTTGCTTATGAAGTGCATACTAAAGTAGGGCTTCATGCAAAAAGTGCTTATGTAAATCGTATTAAAGTACCTT
TGTTAACTGAGCTTAAAAATGGTGATATTGTGCGAGTGGTTACTAGCAATGATAAATTTTATCGCTGTTCTTGGATAGAT
AGTGTTAAAACAGGAAAAGCAAAAGCGAGTATAAGGGAATTTTGTAAGCAAAAAATCAGAGAGATTAATCTAGCAAGTTC
TATTAATATGTTAAGTTTTATTTTTGGAGTTGATAAAAACAGAATTGAAACTTGGATAGAAAAAGAAAATTTAGCTAGAC
GTATTAGACAAGTAGCAACAGATAGCGCTTATTTAAAAGATATAGTCAATGGTCTTAAAAAATACGCCAAGAAATCATAT
TGGTTTGATAAATATGAGATTAAAGAACAAAAAATAGGAAATTTTACTCTATATTGTAACCATAAAATTGCTAATGTGGA
TTTTGATTTTTGCTGCCATCCAAAAAGAGGTGATGCTGTTTTAGCTTTTGTTAAAGGTGGTAACGCTATTGTCCATCATA
AGCTTTGCGATAGAGCTGATAAAATGATAGATAATAATCAAGATATGGTATTTATAAAATGGGATTCTAACATCCCTAAA
TCTTATAAACTGATTTTTTCTTTGGAAAATAAAAAAGGTGTTTTAGCGGAATTTTTGGCATTTTTAGCTAAAATGCAGAT
AAATCTATTAACAATTAATTTATCAAGTGATTTAAATTTGGCAGTAGATTATTTTGAGATTACAATGGAAATTCCTGATA
ATATCAATCCAGATAGTGTCAAGGATAGGTTAAAATCTCGTTGCAAGATTTTAGATTTTACATCTTTAAATGATGCTTAT
AAAGAAGGTTAA

Upstream 100 bases:

>100_bases
GATAAAAATAAAAATAAACTTGCAGATATAGCTTTATATGAAATTGCAGAAAATAAAATCACTTTAGAGGGTTTAGTTGA
AACCAATCGATGAAGAATTA

Downstream 100 bases:

>100_bases
AAAGGTTTATTATGGATATAAAAAAAATATTAGCAGAAGTTAAGCGTGGCTGTGCAGAGCTTATTGATGAAGAAAGAATA
GAAAAATTAATTAAAAATTA

Product: RelA/SpoT family protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPILVATLVGFLSENKSNILAALL
HDVIEDTNCTEEELREQFGSEVLKLVLGLTKIIEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHN
MLTLDILREDKQKRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNEFISKIELL
FLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGILHTHFNPLVSRFKDYIALPKQ
NGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYA
KDSLYVEDVAVYSPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDKFYRCSWID
SVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVATDSAYLKDIVNGLKKYAKKSY
WFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPK
SYKLIFSLENKKGVLAEFLAFLAKMQINLLTINLSSDLNLAVDYFEITMEIPDNINPDSVKDRLKSRCKILDFTSLNDAY
KEG

Sequences:

>Translated_723_residues
MLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPILVATLVGFLSENKSNILAALL
HDVIEDTNCTEEELREQFGSEVLKLVLGLTKIIEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHN
MLTLDILREDKQKRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNEFISKIELL
FLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGILHTHFNPLVSRFKDYIALPKQ
NGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYA
KDSLYVEDVAVYSPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDKFYRCSWID
SVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVATDSAYLKDIVNGLKKYAKKSY
WFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPK
SYKLIFSLENKKGVLAEFLAFLAKMQINLLTINLSSDLNLAVDYFEITMEIPDNINPDSVKDRLKSRCKILDFTSLNDAY
KEG
>Mature_723_residues
MLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPILVATLVGFLSENKSNILAALL
HDVIEDTNCTEEELREQFGSEVLKLVLGLTKIIEIREDNLISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHN
MLTLDILREDKQKRISEETLVVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNEFISKIELL
FLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGILHTHFNPLVSRFKDYIALPKQ
NGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKYKEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYA
KDSLYVEDVAVYSPKGEIFTLPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDKFYRCSWID
SVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVATDSAYLKDIVNGLKKYAKKSY
WFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAVLAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPK
SYKLIFSLENKKGVLAEFLAFLAKMQINLLTINLSSDLNLAVDYFEITMEIPDNINPDSVKDRLKSRCKILDFTSLNDAY
KEG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=651, Percent_Identity=34.1013824884793, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789147, Length=679, Percent_Identity=28.8659793814433, Blast_Score=280, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 82825; Mature: 82825

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPIL
CHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCCCEEEHHHH
VATLVGFLSENKSNILAALLHDVIEDTNCTEEELREQFGSEVLKLVLGLTKIIEIREDNL
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHNMLTLDILREDKQKRISEETL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNEFISKIELL
EEEECHHHHCCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCEEHHCHHHHHHHHHHH
FLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGIL
HHCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTHFNPLVSRFKDYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
KEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYAKDSLYVEDVAVYSPKGEIFT
CCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEE
LPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDKFYRCSWID
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHEECCCCCEEEEEECCCCEEEEEECC
SVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVAT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
DSAYLKDIVNGLKKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAV
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCEEECCCCCEECCCCCCCEE
LAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPKSYKLIFSLENKKGVLAEFLA
EEEEECCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHH
FLAKMQINLLTINLSSDLNLAVDYFEITMEIPDNINPDSVKDRLKSRCKILDFTSLNDAY
HHHHCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCEEEEECCCCHHH
KEG
CCC
>Mature Secondary Structure
MLEQLIDNVKNCKDLEAAKALLFEICGHDAILEKAVDYCIFCHEGQFRKSGEPYAVHPIL
CHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCCCCCCCCEEEHHHH
VATLVGFLSENKSNILAALLHDVIEDTNCTEEELREQFGSEVLKLVLGLTKIIEIREDNL
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ISSKSKKSLTKSALTFRNMLLASIEDVGVLIVKLCDRLHNMLTLDILREDKQKRISEETL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VVYAPIAHRLGISSIKNYLEDLSFKYLMPDEYKLIDNYINSNNQEMQLGLNEFISKIELL
EEEECHHHHCCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCEEHHCHHHHHHHHHHH
FLSNGFRQGSFEIQKRIKHSYSIYLKMQRKGIGIEEVLDLLGVRILVEKVSDCYLALGIL
HHCCCCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
HTHFNPLVSRFKDYIALPKQNGYQTIHTTLFDAKSIIEAQIRTFDMHKIAEFGIAAHWKY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
KEDGSVVAPRLDWLTDISMQSINNLENAEDYNAIELYEYAKDSLYVEDVAVYSPKGEIFT
CCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCEEE
LPRGATVLDFAYEVHTKVGLHAKSAYVNRIKVPLLTELKNGDIVRVVTSNDKFYRCSWID
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHEECCCCCEEEEEECCCCEEEEEECC
SVKTGKAKASIREFCKQKIREINLASSINMLSFIFGVDKNRIETWIEKENLARRIRQVAT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
DSAYLKDIVNGLKKYAKKSYWFDKYEIKEQKIGNFTLYCNHKIANVDFDFCCHPKRGDAV
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCEEECCCCCEECCCCCCCEE
LAFVKGGNAIVHHKLCDRADKMIDNNQDMVFIKWDSNIPKSYKLIFSLENKKGVLAEFLA
EEEEECCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHH
FLAKMQINLLTINLSSDLNLAVDYFEITMEIPDNINPDSVKDRLKSRCKILDFTSLNDAY
HHHHCEEEEEEEEECCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHCEEEEECCCCHHH
KEG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]