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The map label for this gene is gatA [H]

Identifier: 153951202

GI number: 153951202

Start: 601872

End: 603233

Strand: Direct

Name: gatA [H]

Synonym: JJD26997_0663

Alternate gene names: 153951202

Gene position: 601872-603233 (Clockwise)

Preceding gene: 153952173

Following gene: 153952446

Centisome position: 32.62

GC content: 34.14

Gene sequence:

>1362_bases
ATGATAACTTTAAAAGAAGCTTTAAAATACTCCAAAGAAGAACTTGAAAATTTAAAAAAAGAACTCAATGAAAAAGCAAA
AAAAGAAAAAAAATTAGGTGCTTATATCGAACAATTTTTAGATAAAGACTTAAGTGTTTCAGGTGAAGGTGTGCCTGTAG
CTATAAAAGATAATATCAGTGTAAAGGGCTGGGAGCTTACTAGTGCGAGTAAGATTTTACAAGGTTATATTGCTCCTTAT
GATGCAAGTGCGATTGTTAATTTAAAAGCTAATGGTTTTGCTCCTTTTGGGCGTTGTAATATGGATGAATTTGCTATGGG
AAGTTCAACAGCGAGTTCTTGTTATGGTAAAACTTTAAATCCTTTAAATTTTGAGCGTGTTCCAGGTGGATCAAGTGGTG
GAAGTGCTGCAGCGGTGGCGGGTGGCTTGGCTTTGGCAAGTTTGGGTTCTGATACAGGAGGATCTGTACGCCAACCTGCG
GCTTTTTGTGGTTGCGTAGGATTTAAACCCAGTTATGGAAGAGTGAGTCGCTATGGTTTGGCTTCTTATTCTTCGAGTTT
GGATCAAATCGGGGTTTTAACGCAAAATGTAGAAGATGCGGCAATTTTATATGATGCGATCGCAGGATATGATAAAATGG
ATAGCACGAGTGCAAATATCGAGTTTATCAAAACCGCTCCAAATTTAAATGCAAATGAAAAATTAAAAATCGCAGTGATT
GAAAATTATGTTAATGATGCAGATACTGAAGTAAAAAACGCACTTTTAAAAACCATAGATATGTTAAAAGCTAATGGACA
TGAGATTGTATATAAAAATTTACTTGATTCTAAATTTGATATTGCAGCATATTATATCATCGCAACAGCTGAAGCAAGTG
CAAATTTAAGTCGTTATGATGGGGTGCGTTATGGTAAACGTTCTGAAAATATCCAAAATTTAAAAGAAATGTATGTAAAT
ACACGTAGCGAAGGTTTTGGTGAAGAAGTAAAAAGAAGAATTTTGTTAGGAACTTTTGTTTTAAGCAGTGGATATTATGA
TGCGTATTATATCAAAGCACAAAAAGCTAGAGCTTTTATCAAGGCAAAATATGAAGAAATTTTGCAAGATTGTGATCTTA
TTTTTATGCCTGTAACTCCTACAATGGCTTTTAAATTTGATACTCAAAAAAGCCCTATGCAAACTTATTTAGAAGATGTT
TATACTATCTCTGTAAATTTAGCAGGGCTTGGAGGTATTAGTGTGCCTGTTGCAAAAGATAAAGAAGGGCTTAATATATC
AGCACAACTGATTTGCAAAGCTTATGATGAACAAACCTTGCTAGACGGGGCTTTAAGTTTAGAACAAATGATTAAAAATT
AA

Upstream 100 bases:

>100_bases
AAAATCCGATTCCAAATAGTATTATCATTGTAACTATAGCGGTGGTTTTAGCTTTTAGTGCTTTAGCGGTATTTTTGATC
AAAAAAACAAAGGAAAATAA

Downstream 100 bases:

>100_bases
GGATGAAAGATGAAAATTGTAAAAAGAGCTTTAACTTTTGAAGATGTATTATTACGTCCTGGGTATTCTGAAGTTCTACC
TAAGGAAGTAAAAATTCACA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MITLKEALKYSKEELENLKKELNEKAKKEKKLGAYIEQFLDKDLSVSGEGVPVAIKDNISVKGWELTSASKILQGYIAPY
DASAIVNLKANGFAPFGRCNMDEFAMGSSTASSCYGKTLNPLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPA
AFCGCVGFKPSYGRVSRYGLASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANEKLKIAVI
ENYVNDADTEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASANLSRYDGVRYGKRSENIQNLKEMYVN
TRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFIKAKYEEILQDCDLIFMPVTPTMAFKFDTQKSPMQTYLEDV
YTISVNLAGLGGISVPVAKDKEGLNISAQLICKAYDEQTLLDGALSLEQMIKN

Sequences:

>Translated_453_residues
MITLKEALKYSKEELENLKKELNEKAKKEKKLGAYIEQFLDKDLSVSGEGVPVAIKDNISVKGWELTSASKILQGYIAPY
DASAIVNLKANGFAPFGRCNMDEFAMGSSTASSCYGKTLNPLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPA
AFCGCVGFKPSYGRVSRYGLASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANEKLKIAVI
ENYVNDADTEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASANLSRYDGVRYGKRSENIQNLKEMYVN
TRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFIKAKYEEILQDCDLIFMPVTPTMAFKFDTQKSPMQTYLEDV
YTISVNLAGLGGISVPVAKDKEGLNISAQLICKAYDEQTLLDGALSLEQMIKN
>Mature_453_residues
MITLKEALKYSKEELENLKKELNEKAKKEKKLGAYIEQFLDKDLSVSGEGVPVAIKDNISVKGWELTSASKILQGYIAPY
DASAIVNLKANGFAPFGRCNMDEFAMGSSTASSCYGKTLNPLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPA
AFCGCVGFKPSYGRVSRYGLASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANEKLKIAVI
ENYVNDADTEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASANLSRYDGVRYGKRSENIQNLKEMYVN
TRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFIKAKYEEILQDCDLIFMPVTPTMAFKFDTQKSPMQTYLEDV
YTISVNLAGLGGISVPVAKDKEGLNISAQLICKAYDEQTLLDGALSLEQMIKN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=431, Percent_Identity=36.1948955916473, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI195972892, Length=419, Percent_Identity=23.3890214797136, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI166795287, Length=229, Percent_Identity=30.1310043668122, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=411, Percent_Identity=36.2530413625304, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17556264, Length=247, Percent_Identity=33.6032388663968, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=234, Percent_Identity=35.042735042735, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=267, Percent_Identity=26.5917602996255, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=418, Percent_Identity=23.444976076555, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=250, Percent_Identity=30, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=250, Percent_Identity=30, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=274, Percent_Identity=25.5474452554745, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=35.1674641148325, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=399, Percent_Identity=27.0676691729323, Blast_Score=97, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24648113, Length=423, Percent_Identity=36.1702127659575, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24644968, Length=418, Percent_Identity=26.7942583732057, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24652985, Length=456, Percent_Identity=25.219298245614, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922090, Length=456, Percent_Identity=25.219298245614, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652981, Length=456, Percent_Identity=25.219298245614, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24652983, Length=456, Percent_Identity=25.219298245614, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI45550774, Length=130, Percent_Identity=39.2307692307692, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648435, Length=204, Percent_Identity=30.3921568627451, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648437, Length=204, Percent_Identity=30.3921568627451, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648441, Length=204, Percent_Identity=30.3921568627451, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=204, Percent_Identity=30.3921568627451, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI161078093, Length=413, Percent_Identity=26.1501210653753, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356731, Length=180, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49286; Mature: 49286

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKEALKYSKEELENLKKELNEKAKKEKKLGAYIEQFLDKDLSVSGEGVPVAIKDNIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCE
VKGWELTSASKILQGYIAPYDASAIVNLKANGFAPFGRCNMDEFAMGSSTASSCYGKTLN
EEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCHHHHCCCCCC
PLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPAAFCGCVGFKPSYGRVSRYGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHH
ASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANEKLKIAVI
HHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
ENYVNDADTEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASANLSRYD
EHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEEEEEECCCCCCHHHHC
GVRYGKRSENIQNLKEMYVNTRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFI
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
KAKYEEILQDCDLIFMPVTPTMAFKFDTQKSPMQTYLEDVYTISVNLAGLGGISVPVAKD
HHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHCEEEEEEEEEECCCCCCCCCCC
KEGLNISAQLICKAYDEQTLLDGALSLEQMIKN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITLKEALKYSKEELENLKKELNEKAKKEKKLGAYIEQFLDKDLSVSGEGVPVAIKDNIS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCE
VKGWELTSASKILQGYIAPYDASAIVNLKANGFAPFGRCNMDEFAMGSSTASSCYGKTLN
EEEEECCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCHHHHCCCCCC
PLNFERVPGGSSGGSAAAVAGGLALASLGSDTGGSVRQPAAFCGCVGFKPSYGRVSRYGL
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHH
ASYSSSLDQIGVLTQNVEDAAILYDAIAGYDKMDSTSANIEFIKTAPNLNANEKLKIAVI
HHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
ENYVNDADTEVKNALLKTIDMLKANGHEIVYKNLLDSKFDIAAYYIIATAEASANLSRYD
EHHHCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCEEEEEEEEECCCCCCHHHHC
GVRYGKRSENIQNLKEMYVNTRSEGFGEEVKRRILLGTFVLSSGYYDAYYIKAQKARAFI
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
KAKYEEILQDCDLIFMPVTPTMAFKFDTQKSPMQTYLEDVYTISVNLAGLGGISVPVAKD
HHHHHHHHHCCCEEEEECCCCEEEEECCCCCHHHHHHHCEEEEEEEEEECCCCCCCCCCC
KEGLNISAQLICKAYDEQTLLDGALSLEQMIKN
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA