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The map label for this gene is thrS [H]

Identifier: 153950997

GI number: 153950997

Start: 201128

End: 202936

Strand: Direct

Name: thrS [H]

Synonym: JJD26997_0216

Alternate gene names: 153950997

Gene position: 201128-202936 (Clockwise)

Preceding gene: 153952618

Following gene: 153951486

Centisome position: 10.9

GC content: 33.78

Gene sequence:

>1809_bases
ATGGAAAAAGAAGTTATTGCGTATTTGGACAATGAGACAATAATTGACTCTCAAAGTGTGAAAAATACAAATTTAAAAGA
AATTTATTTTGATAATTCTAAAGAAAGTTTAGAAGTGATACGCCATTCTTGTGCGCATTTAATGGCACAGGCGATTAAGA
GCTTGTATCCTGAGGCTAAATTTTTCGTTGGTCCTGTTATAGAAGATGGATTTTATTATGATTTTTGTGTGGAAAGTAAG
ATTGGCGAAGAAGATTTAGTAAAAATTGAAAAAAAGATGAAAGAACTTGCTGAAGCAAAGATTGAAATTTCAAAATACAA
AATCACAAAAAACGAAGCTTTGGCTAAATTTCAAAATGATGATTTAAAACAAGAAGTTTTATTGCGTATTCCTGATGGAG
CTGTAAGCATTTATAAACAAGGTGAATTTGAAGATTTGTGCCGTGGTCCTCATGTGCCAAATACGAAATTTTTACGTTTT
TTTAAACTCACTCGTGTTGCAGGAGCGTATTTAGGTGGCGATGAAAAAAGAGAAATGCTTACTCGAATTTATGGGACTGC
TTTTGCAGATAAGGAAAGTTTAAAAGAGTATTTAGCCATTATTGAAGAAGCTAAAAAACGCGATCATAGAAAGCTTGGAA
TAGAGCTTAAGCTTTTTACTTTTAATGATGAGATCGGAGGAGGTCTTCCTATCTGGTTAGGTAATGGTGCAAGGCTTAGA
TCTAAACTTGAACATATGCTTTATAAGATTCATCGTTTGCGTGGTTATGAGCCTGTGCGTGGGCCTGAGCTTTTAAAAGC
AGATGCATGGAAAATCAGTGGGCATTATGCAAACTATAAAGAAAATATGTATTTTACACAAATTGATGAACAAGAATATG
GCATTAAGCCTATGAACTGTGTGGGGCATATTAAAGTTTATCAAAGTGATGTAAGAAGTTATCGTGATTTGCCTTTGAAA
TTTTTTGAATATGGCGTGGTGCATCGTCATGAAAAAAGTGGGGTTTTGCACGGACTTTTTAGAGTGAGAGAATTCACTCA
AGATGATGCACATATTTTTTGTATGCCAAGTCAGATTAAAGAGCAGGTTTTAGAAATTTTAGCTTTTGTGGATAATTTGA
TGAAATTATTTGATTTTAGTTATGAAATGGAAATTTCAACTAAACCTGAAAAAGCCATAGGTGATGATGAAATTTGGGAA
GTAGCGACTAAGGCTTTAAAAGAAGCTTTAGATGAACAAGGTTTAAAATACGGTATTGATGAAGGTGGTGGGGCTTTTTA
TGGGCCAAAGATCGATATTAAAATCACCGATGCTTTAAAAAGAAAGTGGCAATGCGGAACTATACAAGTAGATTTTAACT
TACCAAGCCGTTTTAAACTCGAATACACTGATAGCGATAATGAAAAAAAACAACCTGTAATGTTGCATCGTGCGATTTTA
GGTTCTTTTGAAAGATTTATAGGAATTTTAACCGAGCATTGTGCGGGTGAGTTTCCATTTTTTATCGCACCAACTGCGGT
GGGTATAGTGCCAATAGGCGAAGCACATATTGCTTACGCAAAAGAAATTCAAAAAGAATTATTAGAGCTTAATATCGATA
GCGAGGTGTATGAAAAAAATGAGAGTTTAAGTAAAAAAATTCGTATCGCTGAAAAACAAAAATTACCTATGATTTTAGTT
TTAGGCGATGATGAGGTGGCAAAGCGTAGCGTTGCTTTAAGAGATAGAAGAGCTAAAGAACAAAAAAATCTTAGCTTGGA
TGAATTTATTAAATTAGTTAAGGAGAAAATGAGTGAGGTATACTTTTGA

Upstream 100 bases:

>100_bases
TAAAAATAAGCTGAATTTCAAAAATAATTAAGTCTTAGAAAGTTAAAATTAGAGCTTTATAAACACTCACAGGGTAAGTG
AGCGACAAAGGGTTAACAAT

Downstream 100 bases:

>100_bases
GTAAAGAAAAAGAAGTATTGCTCAATGAAGAAATAAGAGCGGATGAAATCAGATGTGTTGGCGATGATGGCAAGGTTTAT
GGTATTATCAGTAGTGATGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:

>602_residues
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAKFFVGPVIEDGFYYDFCVESK
IGEEDLVKIEKKMKELAEAKIEISKYKITKNEALAKFQNDDLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRF
FKLTRVAGAYLGGDEKREMLTRIYGTAFADKESLKEYLAIIEEAKKRDHRKLGIELKLFTFNDEIGGGLPIWLGNGARLR
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCVGHIKVYQSDVRSYRDLPLK
FFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIKEQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWE
VATKALKEALDEQGLKYGIDEGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKNESLSKKIRIAEKQKLPMILV
LGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEVYF

Sequences:

>Translated_602_residues
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAKFFVGPVIEDGFYYDFCVESK
IGEEDLVKIEKKMKELAEAKIEISKYKITKNEALAKFQNDDLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRF
FKLTRVAGAYLGGDEKREMLTRIYGTAFADKESLKEYLAIIEEAKKRDHRKLGIELKLFTFNDEIGGGLPIWLGNGARLR
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCVGHIKVYQSDVRSYRDLPLK
FFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIKEQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWE
VATKALKEALDEQGLKYGIDEGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKNESLSKKIRIAEKQKLPMILV
LGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEVYF
>Mature_602_residues
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAKFFVGPVIEDGFYYDFCVESK
IGEEDLVKIEKKMKELAEAKIEISKYKITKNEALAKFQNDDLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRF
FKLTRVAGAYLGGDEKREMLTRIYGTAFADKESLKEYLAIIEEAKKRDHRKLGIELKLFTFNDEIGGGLPIWLGNGARLR
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNCVGHIKVYQSDVRSYRDLPLK
FFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIKEQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWE
VATKALKEALDEQGLKYGIDEGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKNESLSKKIRIAEKQKLPMILV
LGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEVYF

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=599, Percent_Identity=37.3956594323873, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI38570111, Length=583, Percent_Identity=37.5643224699828, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI20070344, Length=595, Percent_Identity=33.4453781512605, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI1788013, Length=603, Percent_Identity=42.7860696517413, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71984184, Length=595, Percent_Identity=38.1512605042017, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71984192, Length=595, Percent_Identity=38.1512605042017, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322112, Length=582, Percent_Identity=39.1752577319588, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6322654, Length=387, Percent_Identity=36.1757105943152, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24583839, Length=572, Percent_Identity=40.7342657342657, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24583842, Length=572, Percent_Identity=40.7342657342657, Blast_Score=444, Evalue=1e-125,
Organism=Drosophila melanogaster, GI28574141, Length=572, Percent_Identity=40.7342657342657, Blast_Score=444, Evalue=1e-125,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 69438; Mature: 69438

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAK
CCCCEEEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
FFVGPVIEDGFYYDFCVESKIGEEDLVKIEKKMKELAEAKIEISKYKITKNEALAKFQND
EEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEHHHEEEEHHHHHHHHCCC
DLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRFFKLTRVAGAYLGGDEKREML
CHHHHHHEECCCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
TRIYGTAFADKESLKEYLAIIEEAKKRDHRKLGIELKLFTFNDEIGGGLPIWLGNGARLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCHHHH
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNC
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEECCCCCCCCCEEEEEECCHHCCCCCCCH
VGHIKVYQSDVRSYRDLPLKFFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIK
HHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCEEEEECHHHH
EQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWEVATKALKEALDEQGLKYGID
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCC
EGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
CCCCEEECCEEEEEEEHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHH
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKN
HHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCC
ESLSKKIRIAEKQKLPMILVLGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEV
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
YF
CC
>Mature Secondary Structure
MEKEVIAYLDNETIIDSQSVKNTNLKEIYFDNSKESLEVIRHSCAHLMAQAIKSLYPEAK
CCCCEEEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
FFVGPVIEDGFYYDFCVESKIGEEDLVKIEKKMKELAEAKIEISKYKITKNEALAKFQND
EEECCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEHHHEEEEHHHHHHHHCCC
DLKQEVLLRIPDGAVSIYKQGEFEDLCRGPHVPNTKFLRFFKLTRVAGAYLGGDEKREML
CHHHHHHEECCCCCEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
TRIYGTAFADKESLKEYLAIIEEAKKRDHRKLGIELKLFTFNDEIGGGLPIWLGNGARLR
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCHHHH
SKLEHMLYKIHRLRGYEPVRGPELLKADAWKISGHYANYKENMYFTQIDEQEYGIKPMNC
HHHHHHHHHHHHHCCCCCCCCCHHHCCCCEEEECCCCCCCCCEEEEEECCHHCCCCCCCH
VGHIKVYQSDVRSYRDLPLKFFEYGVVHRHEKSGVLHGLFRVREFTQDDAHIFCMPSQIK
HHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCHHHHHHHHHHHHCCCCCEEEEECHHHH
EQVLEILAFVDNLMKLFDFSYEMEISTKPEKAIGDDEIWEVATKALKEALDEQGLKYGID
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCC
EGGGAFYGPKIDIKITDALKRKWQCGTIQVDFNLPSRFKLEYTDSDNEKKQPVMLHRAIL
CCCCEEECCEEEEEEEHHHHCCCCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHH
GSFERFIGILTEHCAGEFPFFIAPTAVGIVPIGEAHIAYAKEIQKELLELNIDSEVYEKN
HHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCC
ESLSKKIRIAEKQKLPMILVLGDDEVAKRSVALRDRRAKEQKNLSLDEFIKLVKEKMSEV
HHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA