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The map label for this gene is gspE [H]

Identifier: 153930625

GI number: 153930625

Start: 21911

End: 23443

Strand: Direct

Name: gspE [H]

Synonym: YpsIP31758_A0063

Alternate gene names: 153930625

Gene position: 21911-23443 (Clockwise)

Preceding gene: 153930597

Following gene: 153930581

Centisome position: 37.34

GC content: 41.1

Gene sequence:

>1533_bases
ATGAAAGTTTTAAATTTATCCGGTATTGATGGTGTTGCTGATTATCATTTGCCAGCAAAATTAAAAGATGCAATGTGTCT
GGTCAAAACAGATTTAGGCAATATTGAATTAAGGGTGTCAAGCCACTTAATTAACTCTGCTGAAGTCATGGATTATGAGC
GACAACTAAAAAAAACAAACACGGAATTTAAATTAGTTCCATCCAATATAGCTGAGATTGTAGAGCTTAATCAGCAAAGT
GGGGTAACAACTCAAGAGCAGTTGAATATTATTCACGGTAAAATATTTGAAATTTTTTCGGCGGCAACAGACGCGAGATC
GTCCGATATACATTTTCGTTTACGCAGTAAGTTATGCAATGTCTTTTTTAGAGTTGATGGGAAGTTAAGGTTCTATAAAG
AGTTAACGCCAGAAGATGGTAAGTCATATATTAACTCTCTCTTCAATACACGGTGCGAAGACCGGAGTCATCAGTCAATT
TCCTACCAAGAACCTTGTGGGGCGAAAGTTAGCGAGGATTTTGTACAGCCATTGGGGTTAAGTGGAGGGCGCTTTGCCAG
TCGGCCTGTTGCTGATGGGGGGATGGTTGCTGTTATTCGTCTTATCTCTCGACGAAATGAAATTCTGTCAATGGAACAGC
TGGGTTTAACTGACACTGAAATAGAAACGTTGGAGCGGGCTATAGCAAAGCCTACCGGAGTGATATTTCTAACCGGCCCT
ATGGGATCGGGGAAAAGTACCCTGGCTCAGGTTGTTTGTGAACTGCTCACCAGTCGAGATCCAGGGATACACCTGTTAAC
GGTGGAGAACCCTGTGGAATCCCCTATTAAAGGTGCCGTTCAAACGTCACTTGGAACGGGAGAAGAGTGGTGGGAAGCAA
TTAAAAGCATGATGCGCCTGGATTTGAACTGGATACTGATTGGTGAAGTACGAGATCCCCTTTCTGCTTCAGCAGCTATT
GAAGCGGCTCAAACCGGCCACAATGTACTGACAACAATCCATACCACATTTCCTATTGATACTATTTCTCGGTTAAAAAG
TCTTAATGTACAAAGTGATTTGATTACTGATGCGTCATTGATTACCTGCCTGGTTGGTCAACGACTGGCTCCAAAGCTGT
GTCCCTCATGTAAACAAAAGTACACCGATAGCAGCAAATCGGTTGCACCAGTAATTAAAAAGATTATTGATAAACACTGT
GATATTGACAGTATTTATTTGAAAAACCCTCAAGGATGTACTGAATGCTCAGGGGCTGGAGTAAAAGGCCGAGTGGGTAT
ATTTGAAGTCATTGAAGTCGATGCAAACTTTATGGATATTTATCATAGCCAGGGGAAGATAAAGGCTTATGAACATTGGT
ATATAAATGGTGGTGCCACATTATGTACTAACACTCTTCGTTTAGTTAATACCGGCGAAATCGACCCTGTTTTAAGTCAT
AAAGACGTTTGTAATTTGGATCGCGATTATCTCATCTTTACTGATGAAGTGAGAGCCGCAGCCAAAGTGAATAGAGAGAA
GATAAATGAATAA

Upstream 100 bases:

>100_bases
ACTCTATACCAGGGGGATACAAACTAACAAAAGTAACTCTTGATGAAGTGAGTATTTCTGATGCTGCGGGGACGTCCTTT
ATTCTTAGTGAGGTTGGCTC

Downstream 100 bases:

>100_bases
GTTTAATCGTGCTCTTTATAAAATGACGTTCAATGTTAATGACCGAGAGGATGTTTATGACAATTTCAGACAATATCTTA
TAGATGGTCAGTCTGTTGAT

Product: putative type IV secretion system protein PilQ

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKVLNLSGIDGVADYHLPAKLKDAMCLVKTDLGNIELRVSSHLINSAEVMDYERQLKKTNTEFKLVPSNIAEIVELNQQS
GVTTQEQLNIIHGKIFEIFSAATDARSSDIHFRLRSKLCNVFFRVDGKLRFYKELTPEDGKSYINSLFNTRCEDRSHQSI
SYQEPCGAKVSEDFVQPLGLSGGRFASRPVADGGMVAVIRLISRRNEILSMEQLGLTDTEIETLERAIAKPTGVIFLTGP
MGSGKSTLAQVVCELLTSRDPGIHLLTVENPVESPIKGAVQTSLGTGEEWWEAIKSMMRLDLNWILIGEVRDPLSASAAI
EAAQTGHNVLTTIHTTFPIDTISRLKSLNVQSDLITDASLITCLVGQRLAPKLCPSCKQKYTDSSKSVAPVIKKIIDKHC
DIDSIYLKNPQGCTECSGAGVKGRVGIFEVIEVDANFMDIYHSQGKIKAYEHWYINGGATLCTNTLRLVNTGEIDPVLSH
KDVCNLDRDYLIFTDEVRAAAKVNREKINE

Sequences:

>Translated_510_residues
MKVLNLSGIDGVADYHLPAKLKDAMCLVKTDLGNIELRVSSHLINSAEVMDYERQLKKTNTEFKLVPSNIAEIVELNQQS
GVTTQEQLNIIHGKIFEIFSAATDARSSDIHFRLRSKLCNVFFRVDGKLRFYKELTPEDGKSYINSLFNTRCEDRSHQSI
SYQEPCGAKVSEDFVQPLGLSGGRFASRPVADGGMVAVIRLISRRNEILSMEQLGLTDTEIETLERAIAKPTGVIFLTGP
MGSGKSTLAQVVCELLTSRDPGIHLLTVENPVESPIKGAVQTSLGTGEEWWEAIKSMMRLDLNWILIGEVRDPLSASAAI
EAAQTGHNVLTTIHTTFPIDTISRLKSLNVQSDLITDASLITCLVGQRLAPKLCPSCKQKYTDSSKSVAPVIKKIIDKHC
DIDSIYLKNPQGCTECSGAGVKGRVGIFEVIEVDANFMDIYHSQGKIKAYEHWYINGGATLCTNTLRLVNTGEIDPVLSH
KDVCNLDRDYLIFTDEVRAAAKVNREKINE
>Mature_510_residues
MKVLNLSGIDGVADYHLPAKLKDAMCLVKTDLGNIELRVSSHLINSAEVMDYERQLKKTNTEFKLVPSNIAEIVELNQQS
GVTTQEQLNIIHGKIFEIFSAATDARSSDIHFRLRSKLCNVFFRVDGKLRFYKELTPEDGKSYINSLFNTRCEDRSHQSI
SYQEPCGAKVSEDFVQPLGLSGGRFASRPVADGGMVAVIRLISRRNEILSMEQLGLTDTEIETLERAIAKPTGVIFLTGP
MGSGKSTLAQVVCELLTSRDPGIHLLTVENPVESPIKGAVQTSLGTGEEWWEAIKSMMRLDLNWILIGEVRDPLSASAAI
EAAQTGHNVLTTIHTTFPIDTISRLKSLNVQSDLITDASLITCLVGQRLAPKLCPSCKQKYTDSSKSVAPVIKKIIDKHC
DIDSIYLKNPQGCTECSGAGVKGRVGIFEVIEVDANFMDIYHSQGKIKAYEHWYINGGATLCTNTLRLVNTGEIDPVLSH
KDVCNLDRDYLIFTDEVRAAAKVNREKINE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=388, Percent_Identity=30.4123711340206, Blast_Score=159, Evalue=6e-40,
Organism=Escherichia coli, GI1786296, Length=381, Percent_Identity=28.6089238845144, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI87082188, Length=174, Percent_Identity=35.0574712643678, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 56346; Mature: 56346

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNLSGIDGVADYHLPAKLKDAMCLVKTDLGNIELRVSSHLINSAEVMDYERQLKKTN
CEEEECCCCCCCCCCCCCHHHHHCEEEEEECCCCEEEEEHHHHHCHHHHHHHHHHHHHCC
TEFKLVPSNIAEIVELNQQSGVTTQEQLNIIHGKIFEIFSAATDARSSDIHFRLRSKLCN
CCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VFFRVDGKLRFYKELTPEDGKSYINSLFNTRCEDRSHQSISYQEPCGAKVSEDFVQPLGL
EEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
SGGRFASRPVADGGMVAVIRLISRRNEILSMEQLGLTDTEIETLERAIAKPTGVIFLTGP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECC
MGSGKSTLAQVVCELLTSRDPGIHLLTVENPVESPIKGAVQTSLGTGEEWWEAIKSMMRL
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
DLNWILIGEVRDPLSASAAIEAAQTGHNVLTTIHTTFPIDTISRLKSLNVQSDLITDASL
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHH
ITCLVGQRLAPKLCPSCKQKYTDSSKSVAPVIKKIIDKHCDIDSIYLKNPQGCTECSGAG
HHHHHCCCCCHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
VKGRVGIFEVIEVDANFMDIYHSQGKIKAYEHWYINGGATLCTNTLRLVNTGEIDPVLSH
CCCCCCEEEEEECCCHHHHHHHCCCCEEEEEEEEECCCHHHHHCCEEEEECCCCCCCCCC
KDVCNLDRDYLIFTDEVRAAAKVNREKINE
CCCCCCCCCEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLNLSGIDGVADYHLPAKLKDAMCLVKTDLGNIELRVSSHLINSAEVMDYERQLKKTN
CEEEECCCCCCCCCCCCCHHHHHCEEEEEECCCCEEEEEHHHHHCHHHHHHHHHHHHHCC
TEFKLVPSNIAEIVELNQQSGVTTQEQLNIIHGKIFEIFSAATDARSSDIHFRLRSKLCN
CCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VFFRVDGKLRFYKELTPEDGKSYINSLFNTRCEDRSHQSISYQEPCGAKVSEDFVQPLGL
EEEEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
SGGRFASRPVADGGMVAVIRLISRRNEILSMEQLGLTDTEIETLERAIAKPTGVIFLTGP
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEECC
MGSGKSTLAQVVCELLTSRDPGIHLLTVENPVESPIKGAVQTSLGTGEEWWEAIKSMMRL
CCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHC
DLNWILIGEVRDPLSASAAIEAAQTGHNVLTTIHTTFPIDTISRLKSLNVQSDLITDASL
CCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHH
ITCLVGQRLAPKLCPSCKQKYTDSSKSVAPVIKKIIDKHCDIDSIYLKNPQGCTECSGAG
HHHHHCCCCCHHCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
VKGRVGIFEVIEVDANFMDIYHSQGKIKAYEHWYINGGATLCTNTLRLVNTGEIDPVLSH
CCCCCCEEEEEECCCHHHHHHHCCCCEEEEEEEEECCCHHHHHCCEEEEECCCCCCCCCC
KDVCNLDRDYLIFTDEVRAAAKVNREKINE
CCCCCCCCCEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]