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The map label for this gene is topB [H]

Identifier: 153930584

GI number: 153930584

Start: 42867

End: 44906

Strand: Reverse

Name: topB [H]

Synonym: YpsIP31758_A0023

Alternate gene names: 153930584

Gene position: 44906-42867 (Counterclockwise)

Preceding gene: 153930620

Following gene: 153930582

Centisome position: 76.53

GC content: 36.52

Gene sequence:

>2040_bases
ATGAGTGATATAAAACGGTTATTTATTGCTGAAAAACCAAACGTAGCAAGAGCGATTGCCGCTGAACTAGGAATTACAGG
TAAAGGGAATGGTTATATAAAATGTGGTTCGGATACCATAACATGGTGTTTTGGGCATATGTTTGAACAAGCTGAACCTG
ATGAATATACACCTGATGATGTACCAAGAACCCCTAAAGGAAATAAAATTTGGCGAGAGAATGAGTTACCAATAATTCCT
GAAGAATGGATAATTAAACCAAGAGAAGATGCCGAATCTCAATTGAAAGTGATTTCAGGTTTCTTGAAAGAAGCCGACTT
GATTGTTAATGCAGGTGATCCTGATCGCGAAGGGCAGCTATTGGTTGATGAATTGTTAGAATATTATAATTGTAATCTAC
CAGTTAAGCGTTATTGGGTTTCATCTCAAGACTCTGTTGCTGTTAAACGTGGTTTAGCCGATCTGAAAGATAATAAGCAA
TTTTATGGGTATGGTGTAGCAGCCTTAGCTCGCGGACGGTGTGACTGGTTAATTGGTATGAATTTGAGCCGAGCATATAC
GTTACGGGCTAAGCGCGGTGGCTATGCTGTTTTACTGTCAGTTGGACGAGTCCAAACTCCTGTTTTATCGCTAGTTGTAG
GGAGGGATAGAGAAATAGAAGCTTTTAAGCCTATTCCTTTTTATAAAATCAAAGCCGCTGTTAAATGCAATGATAGCGCA
TTTGTAACTGATTGGATCGCCAAGGAAGACCAGGAGGGGTTAGACAGTGAAGGCCGTTTGGTCGATAGAAATATAGCTTC
AAAATTGGCAGATAAGTTAAATGAAAAAACGGGAGAAATAAAAGAATATAAGAAGACTCCACAGAAAAAGCTCCACCCTT
TTGGATTTTCTTTATCAAGTCTTACTGTAGTTGCTTCAAATAAATTTGGTTATAGCTCTGCTGATGTTTTGGAAATATGT
CAATCATTATATGAAACTCATAAACTAACTAGCTATCCCCGTTCAGATTGTAAATTTTTGCCTGAAACACAATTTGCGGA
TGCTCATGCTGTCCTATCTGCAATTAAGCATGTTAATCCAGATAAGGCTGATTTAGTTGATGCTGTTGATATTAAAATAA
AATCAAGGATATGGAATGATAATGAAGTGAGTGCTCATCATGCAATTATTCCAACTATGCATATGGGTTCTGCGGATAGA
TTAAATGTAAAAGAGAAGAACATATACGATTTAATAGTCAGGTCGTATTTGGCTCAGTTTTATCCTGTACATGAATTTCT
TAAAACAACCGTGTCAATTGATATTGATACTGAAACTTTTAAAGCTGAGGGTAATACAGTTACACACGCCGGATGGAAGA
GTATTTATACAGAAAGTGAAGAGGACGATGAAAAAAATGCAGTGCAAACTTTTGTGGTAATGAAAAAAGGAGATACTGTC
ACTTGCTTAAAATCTTCATTAGTAGATTCAAAAACTAAAGCACCACCTAGATTTAATGATGGTTCTTTGATTGCTGCAAT
GGAAAATATTCATAAATATGTTCCTGATTCAGAATTAAAAAAATTACTGAGAGATGGTGATGGTATTGGTACTGTTGCCA
CAAGGCCATTTATTATTGAAGAGTTGAAAAATAAAGGTTATTTGGAGTTAAAAGGTAAGCAATTAATTAGCACTACTTTA
GGCAGAAGTATCATAGATATTTATCCTGAAGTGATGAAAAGCGCAGTTTTAACAGCATTAAATGAGCGTGTATTTATTGA
TATACAAAAAGAACCATCGGGAATAGATGATTTTCTAGAAAAACAAATCAAGTTTATTACTGACCAAGTAGAAAAAGCAG
GTAATGGCGTAGCCAAAGTTTCTGGCGCTAAACAGGTTGCCAAAATATCGACCATTCATAAATGCAAATCTTGCGGAAAA
GGGCTTGTTCACAGAACCGGAAAAAAAGGGTTGTGGTGGTCATGCAGTGGTTATCCCGAATGTAATCAGTCCTACCCTGA
TGCAAAAGGAAAGCCTAACTATACCAAAGGTAAGGAGTAA

Upstream 100 bases:

>100_bases
ATTTCGGTTTTATTGATAAACAACAAATACACAGTGTTATTCGTCCAATATTAAGTTGGAAGTAACATGTTAGATATTTA
CTTTAAAGGATTTTAAATAT

Downstream 100 bases:

>100_bases
TCGTTATGAACCAAAAAACAAACTATCCAGATGTTGCGGTTTTTAATAATGAAAAGGATTCATCCTCTGTATTAAATGAA
AAGTTAAATGATGCTTATAC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MSDIKRLFIAEKPNVARAIAAELGITGKGNGYIKCGSDTITWCFGHMFEQAEPDEYTPDDVPRTPKGNKIWRENELPIIP
EEWIIKPREDAESQLKVISGFLKEADLIVNAGDPDREGQLLVDELLEYYNCNLPVKRYWVSSQDSVAVKRGLADLKDNKQ
FYGYGVAALARGRCDWLIGMNLSRAYTLRAKRGGYAVLLSVGRVQTPVLSLVVGRDREIEAFKPIPFYKIKAAVKCNDSA
FVTDWIAKEDQEGLDSEGRLVDRNIASKLADKLNEKTGEIKEYKKTPQKKLHPFGFSLSSLTVVASNKFGYSSADVLEIC
QSLYETHKLTSYPRSDCKFLPETQFADAHAVLSAIKHVNPDKADLVDAVDIKIKSRIWNDNEVSAHHAIIPTMHMGSADR
LNVKEKNIYDLIVRSYLAQFYPVHEFLKTTVSIDIDTETFKAEGNTVTHAGWKSIYTESEEDDEKNAVQTFVVMKKGDTV
TCLKSSLVDSKTKAPPRFNDGSLIAAMENIHKYVPDSELKKLLRDGDGIGTVATRPFIIEELKNKGYLELKGKQLISTTL
GRSIIDIYPEVMKSAVLTALNERVFIDIQKEPSGIDDFLEKQIKFITDQVEKAGNGVAKVSGAKQVAKISTIHKCKSCGK
GLVHRTGKKGLWWSCSGYPECNQSYPDAKGKPNYTKGKE

Sequences:

>Translated_679_residues
MSDIKRLFIAEKPNVARAIAAELGITGKGNGYIKCGSDTITWCFGHMFEQAEPDEYTPDDVPRTPKGNKIWRENELPIIP
EEWIIKPREDAESQLKVISGFLKEADLIVNAGDPDREGQLLVDELLEYYNCNLPVKRYWVSSQDSVAVKRGLADLKDNKQ
FYGYGVAALARGRCDWLIGMNLSRAYTLRAKRGGYAVLLSVGRVQTPVLSLVVGRDREIEAFKPIPFYKIKAAVKCNDSA
FVTDWIAKEDQEGLDSEGRLVDRNIASKLADKLNEKTGEIKEYKKTPQKKLHPFGFSLSSLTVVASNKFGYSSADVLEIC
QSLYETHKLTSYPRSDCKFLPETQFADAHAVLSAIKHVNPDKADLVDAVDIKIKSRIWNDNEVSAHHAIIPTMHMGSADR
LNVKEKNIYDLIVRSYLAQFYPVHEFLKTTVSIDIDTETFKAEGNTVTHAGWKSIYTESEEDDEKNAVQTFVVMKKGDTV
TCLKSSLVDSKTKAPPRFNDGSLIAAMENIHKYVPDSELKKLLRDGDGIGTVATRPFIIEELKNKGYLELKGKQLISTTL
GRSIIDIYPEVMKSAVLTALNERVFIDIQKEPSGIDDFLEKQIKFITDQVEKAGNGVAKVSGAKQVAKISTIHKCKSCGK
GLVHRTGKKGLWWSCSGYPECNQSYPDAKGKPNYTKGKE
>Mature_678_residues
SDIKRLFIAEKPNVARAIAAELGITGKGNGYIKCGSDTITWCFGHMFEQAEPDEYTPDDVPRTPKGNKIWRENELPIIPE
EWIIKPREDAESQLKVISGFLKEADLIVNAGDPDREGQLLVDELLEYYNCNLPVKRYWVSSQDSVAVKRGLADLKDNKQF
YGYGVAALARGRCDWLIGMNLSRAYTLRAKRGGYAVLLSVGRVQTPVLSLVVGRDREIEAFKPIPFYKIKAAVKCNDSAF
VTDWIAKEDQEGLDSEGRLVDRNIASKLADKLNEKTGEIKEYKKTPQKKLHPFGFSLSSLTVVASNKFGYSSADVLEICQ
SLYETHKLTSYPRSDCKFLPETQFADAHAVLSAIKHVNPDKADLVDAVDIKIKSRIWNDNEVSAHHAIIPTMHMGSADRL
NVKEKNIYDLIVRSYLAQFYPVHEFLKTTVSIDIDTETFKAEGNTVTHAGWKSIYTESEEDDEKNAVQTFVVMKKGDTVT
CLKSSLVDSKTKAPPRFNDGSLIAAMENIHKYVPDSELKKLLRDGDGIGTVATRPFIIEELKNKGYLELKGKQLISTTLG
RSIIDIYPEVMKSAVLTALNERVFIDIQKEPSGIDDFLEKQIKFITDQVEKAGNGVAKVSGAKQVAKISTIHKCKSCGKG
LVHRTGKKGLWWSCSGYPECNQSYPDAKGKPNYTKGKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=599, Percent_Identity=25.542570951586, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI4507635, Length=712, Percent_Identity=22.3314606741573, Blast_Score=107, Evalue=4e-23,
Organism=Escherichia coli, GI1788061, Length=616, Percent_Identity=42.2077922077922, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787529, Length=582, Percent_Identity=23.0240549828179, Blast_Score=115, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17555378, Length=625, Percent_Identity=25.28, Blast_Score=107, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=648, Percent_Identity=22.9938271604938, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585251, Length=600, Percent_Identity=25, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24640096, Length=448, Percent_Identity=24.3303571428571, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 75840; Mature: 75709

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIKRLFIAEKPNVARAIAAELGITGKGNGYIKCGSDTITWCFGHMFEQAEPDEYTPDD
CCCHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
VPRTPKGNKIWRENELPIIPEEWIIKPREDAESQLKVISGFLKEADLIVNAGDPDREGQL
CCCCCCCCCCEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCH
LVDELLEYYNCNLPVKRYWVSSQDSVAVKRGLADLKDNKQFYGYGVAALARGRCDWLIGM
HHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCEEECC
NLSRAYTLRAKRGGYAVLLSVGRVQTPVLSLVVGRDREIEAFKPIPFYKIKAAVKCNDSA
CCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCC
FVTDWIAKEDQEGLDSEGRLVDRNIASKLADKLNEKTGEIKEYKKTPQKKLHPFGFSLSS
EEHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCHHH
LTVVASNKFGYSSADVLEICQSLYETHKLTSYPRSDCKFLPETQFADAHAVLSAIKHVNP
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
DKADLVDAVDIKIKSRIWNDNEVSAHHAIIPTMHMGSADRLNVKEKNIYDLIVRSYLAQF
CHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
YPVHEFLKTTVSIDIDTETFKAEGNTVTHAGWKSIYTESEEDDEKNAVQTFVVMKKGDTV
HHHHHHHHHHEEEEECCCEEECCCCEEEECCHHHHCCCCCCCHHHHHEEEEEEEECCCCH
TCLKSSLVDSKTKAPPRFNDGSLIAAMENIHKYVPDSELKKLLRDGDGIGTVATRPFIIE
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
ELKNKGYLELKGKQLISTTLGRSIIDIYPEVMKSAVLTALNERVFIDIQKEPSGIDDFLE
HHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
KQIKFITDQVEKAGNGVAKVSGAKQVAKISTIHKCKSCGKGLVHRTGKKGLWWSCSGYPE
HHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEECCCCCC
CNQSYPDAKGKPNYTKGKE
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDIKRLFIAEKPNVARAIAAELGITGKGNGYIKCGSDTITWCFGHMFEQAEPDEYTPDD
CCHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
VPRTPKGNKIWRENELPIIPEEWIIKPREDAESQLKVISGFLKEADLIVNAGDPDREGQL
CCCCCCCCCCEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCH
LVDELLEYYNCNLPVKRYWVSSQDSVAVKRGLADLKDNKQFYGYGVAALARGRCDWLIGM
HHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCEEECC
NLSRAYTLRAKRGGYAVLLSVGRVQTPVLSLVVGRDREIEAFKPIPFYKIKAAVKCNDSA
CCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCC
FVTDWIAKEDQEGLDSEGRLVDRNIASKLADKLNEKTGEIKEYKKTPQKKLHPFGFSLSS
EEHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHCCHHHHCCCCCCCHHH
LTVVASNKFGYSSADVLEICQSLYETHKLTSYPRSDCKFLPETQFADAHAVLSAIKHVNP
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
DKADLVDAVDIKIKSRIWNDNEVSAHHAIIPTMHMGSADRLNVKEKNIYDLIVRSYLAQF
CHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
YPVHEFLKTTVSIDIDTETFKAEGNTVTHAGWKSIYTESEEDDEKNAVQTFVVMKKGDTV
HHHHHHHHHHEEEEECCCEEECCCCEEEECCHHHHCCCCCCCHHHHHEEEEEEEECCCCH
TCLKSSLVDSKTKAPPRFNDGSLIAAMENIHKYVPDSELKKLLRDGDGIGTVATRPFIIE
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
ELKNKGYLELKGKQLISTTLGRSIIDIYPEVMKSAVLTALNERVFIDIQKEPSGIDDFLE
HHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHH
KQIKFITDQVEKAGNGVAKVSGAKQVAKISTIHKCKSCGKGLVHRTGKKGLWWSCSGYPE
HHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCEEECCCCCC
CNQSYPDAKGKPNYTKGKE
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]