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The map label for this gene is traF [H]

Identifier: 153930620

GI number: 153930620

Start: 44942

End: 45466

Strand: Reverse

Name: traF [H]

Synonym: YpsIP31758_A0024

Alternate gene names: 153930620

Gene position: 45466-44942 (Counterclockwise)

Preceding gene: 153930564

Following gene: 153930584

Centisome position: 77.48

GC content: 34.48

Gene sequence:

>525_bases
ATGAAACAGTCACACTCGTTGGCTTTTGTAGTTAGCGGAATAGCTTTGATATCTACAATTATTTATATCTCTGGTATTCG
AGTTAACACAAGTGAAAGTATTCCCGTAGGATTATATTTAATGACACCGTCACCTATCTCTAAAGGAGAGTATGTTGTTT
TTTGTCCTCCACCGACAAGTGATTTTGATAAAGCTAGAAGTCGAGGTTATATCAACTCTGGTTTCTGCTCAGGTAACTAT
GGTTATATGATGAAGAGAATTTTAGCCGCTAAAGGAGATGTTGTTTCTATTAATAGTAATGGTGTGATTGTTAATAATAT
TAAAATACCCTATAGCAAGCCTCTCTCTGTTGATTTGTCAGGGAACCCACTGCCTAGCATTAATATACTCAATCATACAT
TGCAAGAATCAGAGCTTCTTTTAATGACTGACCAAAGTAATAGTTCTTTTGATTCGCGATATTTCGGTTTTATTGATAAA
CAACAAATACACAGTGTTATTCGTCCAATATTAAGTTGGAAGTAA

Upstream 100 bases:

>100_bases
AAGACCCTATTTTTACTGCCCGAGCTGCCGTACCTGAACCAAAATTTAGTGACAAGATTAGAGGAAACTTACTCCTTACA
GAAGGGGAGGTTATCGCTTT

Downstream 100 bases:

>100_bases
CATGTTAGATATTTACTTTAAAGGATTTTAAATATATGAGTGATATAAAACGGTTATTTATTGCTGAAAAACCAAACGTA
GCAAGAGCGATTGCCGCTGA

Product: conjugal transfer peptidase TraF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKQSHSLAFVVSGIALISTIIYISGIRVNTSESIPVGLYLMTPSPISKGEYVVFCPPPTSDFDKARSRGYINSGFCSGNY
GYMMKRILAAKGDVVSINSNGVIVNNIKIPYSKPLSVDLSGNPLPSINILNHTLQESELLLMTDQSNSSFDSRYFGFIDK
QQIHSVIRPILSWK

Sequences:

>Translated_174_residues
MKQSHSLAFVVSGIALISTIIYISGIRVNTSESIPVGLYLMTPSPISKGEYVVFCPPPTSDFDKARSRGYINSGFCSGNY
GYMMKRILAAKGDVVSINSNGVIVNNIKIPYSKPLSVDLSGNPLPSINILNHTLQESELLLMTDQSNSSFDSRYFGFIDK
QQIHSVIRPILSWK
>Mature_174_residues
MKQSHSLAFVVSGIALISTIIYISGIRVNTSESIPVGLYLMTPSPISKGEYVVFCPPPTSDFDKARSRGYINSGFCSGNY
GYMMKRILAAKGDVVSINSNGVIVNNIKIPYSKPLSVDLSGNPLPSINILNHTLQESELLLMTDQSNSSFDSRYFGFIDK
QQIHSVIRPILSWK

Specific function: Required for donor-specific phage sensitivity. May be involved in pilus assembly [H]

COG id: COG4959

COG function: function code OU; Type IV secretory pathway, protease TraF

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR014139 [H]

Pfam domain/function: PF10502 Peptidase_S26 [H]

EC number: NA

Molecular weight: Translated: 19138; Mature: 19138

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSHSLAFVVSGIALISTIIYISGIRVNTSESIPVGLYLMTPSPISKGEYVVFCPPPTS
CCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCC
DFDKARSRGYINSGFCSGNYGYMMKRILAAKGDVVSINSNGVIVNNIKIPYSKPLSVDLS
HHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCEEEEEC
GNPLPSINILNHTLQESELLLMTDQSNSSFDSRYFGFIDKQQIHSVIRPILSWK
CCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQSHSLAFVVSGIALISTIIYISGIRVNTSESIPVGLYLMTPSPISKGEYVVFCPPPTS
CCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCC
DFDKARSRGYINSGFCSGNYGYMMKRILAAKGDVVSINSNGVIVNNIKIPYSKPLSVDLS
HHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCCCEEEEEC
GNPLPSINILNHTLQESELLLMTDQSNSSFDSRYFGFIDKQQIHSVIRPILSWK
CCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400217 [H]