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The map label for this gene is def-1 [H]

Identifier: 153937608

GI number: 153937608

Start: 2346673

End: 2347125

Strand: Reverse

Name: def-1 [H]

Synonym: CLC_2217

Alternate gene names: 153937608

Gene position: 2347125-2346673 (Counterclockwise)

Preceding gene: 153935529

Following gene: 153937249

Centisome position: 62.41

GC content: 30.68

Gene sequence:

>453_bases
ATGGCATTAAGACAAATTAGATTGTTTGATGATGAAATATTAAGAAAAGAGAGTAAGGTAGTTGAGATTGTAGACGATAA
AATAAGACAAATATTAAATGATATGGCAGATACTATGTATAATACAGAAAATGGAGGAGGATTAGCTGCCCCACAAGTAG
GTATATTGAAGCGATTAGTTGTAATAGATATGGGACAAGGACTTATAAAATTAGTAAATCCAAAGATAATTAAACAAGAA
GGAACTCAAGAAGTTATTGAAGGGTGTCTGAGTATTCCTAATAAATTTGGAAGGGTAATAAGACCTGCAAAAGTAACAGT
ACAAGCATTAAATGAAGATGGAGAGGAAATTATATTAACAGGTACAGGAGACTTAGCAAAATGTTTTTGCCATGAAATAG
ACCATTTAGAAGGTATTCTTTTTACTGATTTAGTAACTGAATACATAATCTAA

Upstream 100 bases:

>100_bases
TGATATTGCCAAAGTATTCCATGACACTATAGAAGAAGTTTTCCTTTTTGAAGAAAGTGAAAGAAAATCAAGATCAGAGA
AAAGTAGAGGTGTAGTTTAA

Downstream 100 bases:

>100_bases
TATCATTTATATTATAAATTTATTTGATTGCAAAAACTTGCGCTAGGAAAAGAAATCTCTTTTCTTATTAGTATATTAAA
AAAACAAATAATTTTAACAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMADTMYNTENGGGLAAPQVGILKRLVVIDMGQGLIKLVNPKIIKQE
GTQEVIEGCLSIPNKFGRVIRPAKVTVQALNEDGEEIILTGTGDLAKCFCHEIDHLEGILFTDLVTEYII

Sequences:

>Translated_150_residues
MALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMADTMYNTENGGGLAAPQVGILKRLVVIDMGQGLIKLVNPKIIKQE
GTQEVIEGCLSIPNKFGRVIRPAKVTVQALNEDGEEIILTGTGDLAKCFCHEIDHLEGILFTDLVTEYII
>Mature_149_residues
ALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMADTMYNTENGGGLAAPQVGILKRLVVIDMGQGLIKLVNPKIIKQEG
TQEVIEGCLSIPNKFGRVIRPAKVTVQALNEDGEEIILTGTGDLAKCFCHEIDHLEGILFTDLVTEYII

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=42.9530201342282, Blast_Score=110, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 16704; Mature: 16573

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMADTMYNTENGGGLAAPQVGILKRLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VIDMGQGLIKLVNPKIIKQEGTQEVIEGCLSIPNKFGRVIRPAKVTVQALNEDGEEIILT
HHHCCCHHHHHHCCHHHHCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCCEEEEE
GTGDLAKCFCHEIDHLEGILFTDLVTEYII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALRQIRLFDDEILRKESKVVEIVDDKIRQILNDMADTMYNTENGGGLAAPQVGILKRLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
VIDMGQGLIKLVNPKIIKQEGTQEVIEGCLSIPNKFGRVIRPAKVTVQALNEDGEEIILT
HHHCCCHHHHHHCCHHHHCCCHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCCEEEEE
GTGDLAKCFCHEIDHLEGILFTDLVTEYII
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]