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The map label for this gene is ksgA

Identifier: 153937047

GI number: 153937047

Start: 104641

End: 105468

Strand: Direct

Name: ksgA

Synonym: CLC_0128

Alternate gene names: 153937047

Gene position: 104641-105468 (Clockwise)

Preceding gene: 153936070

Following gene: 153937636

Centisome position: 2.78

GC content: 27.54

Gene sequence:

>828_bases
ATGAATACAAAGGAAATAGTTAACAAATATGAATTTAAGTTTAATAAGAATTTAGGACAAAACTTTTTAATAGATGAATC
TGTATTGGAAGATATAATAGAGGGAGCTGAAATAAACAAAGAAGATACTGTTATAGAGATAGGCCCTGGGGTTGGGACTT
TAACTAAGGAGCTTTTAGAAAGAGCAAAAGAAGTTTACTCTATAGAATTAGATGGTGATTTAATACCTATTTTGCAAGAG
GAGTTAAAGGAATATAATAATTTTACACTAATACATAAAGATGCGTTAAAAATTGATTTTAATGAGTTAATGGAAAATAA
GGATTCCATAAAATTAGTAGCCAATCTACCTTATTATGTAACTACACCTATTATATCAAGATTACTTACAGAAAAATGTG
ATTTTAAATCACTAACCATAATGATTCAAAAGGAAGTAGCGGAAAGAATAAATGCTGAACCTAACTGTAAGGAGTATGGT
TCATTAACCGTTTTAGTACAATATTATTGTAATACAAAAATAATAAGAAAAGTATCTCCAAATTCTTTTATACCAAGACC
TAAAGTGGATTCTATAGTTATAAAATTAGATAGACTTTCAGAACCAAGAGTAAGGGTGAAAAGCCAAAAATTATTTTTTA
ATGTAGTAAGAAGTTCATTTAATATGAGAAGAAAAACTTTATGGAATTCATTAAAAAGTTTAAATATAGATAAAGAAAGT
ATGGAAAATGCTTTTGAAAGAGCAGGGATAGATCCAAAACGAAGGGGCGAGACTTTATCCATAGAAGAATTCGGAAAACT
TTCAGATTGTATATATGATATATTATAA

Upstream 100 bases:

>100_bases
TGCAATACTAATCAGTTTATAAAAAGACTTAACAATTTTGGTATAACCAAAGAAGAGTTTATAAAGGCTATAAAAGATTT
AGATAAGGGGAATGAATAAT

Downstream 100 bases:

>100_bases
TTTAAATAATTAAGTTCACTTTTTGTAAGATAACTCATATATTATATTGAATAATTATATTTGGGGGTACAATAAAAAGT
GGCACAAAAAAAGAATTATA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPILQE
ELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYG
SLTVLVQYYCNTKIIRKVSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL

Sequences:

>Translated_275_residues
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPILQE
ELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYG
SLTVLVQYYCNTKIIRKVSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
>Mature_275_residues
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLERAKEVYSIELDGDLIPILQE
ELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYVTTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYG
SLTVLVQYYCNTKIIRKVSPNSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=288, Percent_Identity=30.5555555555556, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=32.7354260089686, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786236, Length=257, Percent_Identity=35.7976653696498, Blast_Score=167, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI25141369, Length=293, Percent_Identity=26.962457337884, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=197, Percent_Identity=33.502538071066, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=227, Percent_Identity=31.7180616740088, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358017, Length=201, Percent_Identity=34.3283582089552, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21357273, Length=284, Percent_Identity=28.5211267605634, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CLOB1 (A7FQA9)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382487.1
- STRING:   A7FQA9
- GeneID:   5396459
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0116
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- BioCyc:   CBOT441770:CLB_0116-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31813; Mature: 31813

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 21-21 BINDING 23-23 BINDING 48-48 BINDING 69-69 BINDING 94-94 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLE
CCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
RAKEVYSIELDGDLIPILQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYV
HHHHHEEEEECCCCHHHHHHHHHHHCCEEEEECCCCEECHHHHHCCCCCEEEEECCCHHH
TTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYGSLTVLVQYYCNTKIIRKVSP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEHHHCCCEEEECCC
NSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
HHHHHHHCCCCHHHCCCEECHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTKEIVNKYEFKFNKNLGQNFLIDESVLEDIIEGAEINKEDTVIEIGPGVGTLTKELLE
CCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHH
RAKEVYSIELDGDLIPILQEELKEYNNFTLIHKDALKIDFNELMENKDSIKLVANLPYYV
HHHHHEEEEECCCCHHHHHHHHHHHCCEEEEECCCCEECHHHHHCCCCCEEEEECCCHHH
TTPIISRLLTEKCDFKSLTIMIQKEVAERINAEPNCKEYGSLTVLVQYYCNTKIIRKVSP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEHHHCCCEEEECCC
NSFIPRPKVDSIVIKLDRLSEPRVRVKSQKLFFNVVRSSFNMRRKTLWNSLKSLNIDKES
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MENAFERAGIDPKRRGETLSIEEFGKLSDCIYDIL
HHHHHHHCCCCHHHCCCEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA