Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 153936781

GI number: 153936781

Start: 775435

End: 775827

Strand: Direct

Name: gcvH

Synonym: CLC_0751

Alternate gene names: 153936781

Gene position: 775435-775827 (Clockwise)

Preceding gene: 153934598

Following gene: 153934481

Centisome position: 20.62

GC content: 33.59

Gene sequence:

>393_bases
ATGAAGGTTTTAAATAATTTACTTTACACTGGTGATCATGAATGGGTAAGGGTAGAAGATAACAAAGCATATATAGGAAT
CAGCGATTGTGCACAACGTATGTTAAGTGATATAGTTTTTGTTGAATTACCAGAGGTTGATGATGAAATAGCAAAGGGTG
AAACTTTTGCAACTATAGAATCTGTTAAAGCAGCATCAGACAGTTACATGCCTGTTAGTGGAACAATTGTAGAAATTAAT
GAAGAACTAGAAGATAATCCAGCAGCATTAAACGAAGACCCATATGGAAGCTGGATTGCTGCAATAGAGATGTCAGATAA
AAGTGAATTAGAAGAATTAATAAAACCAGAAGTTTATGAGAAAATATGTGAAGAGTTAGATAAGGAGGCATAA

Upstream 100 bases:

>100_bases
TATTTTTTAATATATATAAATTCACACTAATGTTTAGTTTTCAGGCTAAAATGTCATTGGTAATCAATATATAATAATTT
AAAAATTGGGGGTTAGTAGA

Downstream 100 bases:

>100_bases
TTATGTTTCCATATATTCCAAACACAGAAAAAGAAACAAAAGAAATTTTAGATTTTCTTTCAATAAAATCTGTATATGAA
TTATTTAGTGATATACCAGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIAAIEMSDKSELEELIKPEVYEKICEELDKEA

Sequences:

>Translated_130_residues
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIAAIEMSDKSELEELIKPEVYEKICEELDKEA
>Mature_130_residues
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIAAIEMSDKSELEELIKPEVYEKICEELDKEA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=44.7368421052632, Blast_Score=102, Evalue=7e-23,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=43.859649122807, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=48.7603305785124, Blast_Score=113, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=45.0819672131148, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.1034482758621, Blast_Score=104, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=44.1666666666667, Blast_Score=104, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=42.1052631578947, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_CLOB1 (A7FRV4)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383081.1
- ProteinModelPortal:   A7FRV4
- SMR:   A7FRV4
- STRING:   A7FRV4
- GeneID:   5397522
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0736
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- BioCyc:   CBOT441770:CLB_0736-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14605; Mature: 14605

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIE
CCHHHCCEECCCCCEEEEECCCEEEEHHHHHHHHHHHHEEEECCCCCHHHHCCCHHHHHH
SVKAASDSYMPVSGTIVEINEELEDNPAALNEDPYGSWIAAIEMSDKSELEELIKPEVYE
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCHHHHH
KICEELDKEA
HHHHHHCCCC
>Mature Secondary Structure
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIE
CCHHHCCEECCCCCEEEEECCCEEEEHHHHHHHHHHHHEEEECCCCCHHHHCCCHHHHHH
SVKAASDSYMPVSGTIVEINEELEDNPAALNEDPYGSWIAAIEMSDKSELEELIKPEVYE
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCHHHHH
KICEELDKEA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA