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The map label for this gene is gcvT

Identifier: 153934598

GI number: 153934598

Start: 774198

End: 775310

Strand: Direct

Name: gcvT

Synonym: CLC_0750

Alternate gene names: 153934598

Gene position: 774198-775310 (Clockwise)

Preceding gene: 153935749

Following gene: 153936781

Centisome position: 20.59

GC content: 30.73

Gene sequence:

>1113_bases
ATGAAAGATTTAAAAGTTACGCCTTTAAGAGGAGTGTATGAAGAGTATGGTGGGAAAATTGTAGATTTTGCAGGATATGA
ACTTCCAACACAATTTAAAGGCTTTTTACATGAGCATCATACTGTAAGAGAAAAAGCAGGTCTTTTTGATGTTTCTCATA
TGGGAGAAGCTATGGTAACAGGAAAAGATGCTGGAAAATTCATTCAATACTTAATGACTAATGACATAAATGTTCTTAAA
GATAATGAGGTTTTGTACACATTCATGTGTAATGAAGATGGTGGAGTGATAGATGATTTATTGGTTTATAAATTTGCAGA
GGATGAGTTTTTTTTAGTTATTAATGCAAGTAATAAGGATAAAGATGTTAAGTGGATAATGGACCACAAAGGGGATTTTG
ATGTTGAAATTGTTGATGTATCAGATAGCATTGCACAACTTGCATTCCAAGGACCTTTAGCAGAAGAAATACTTCAAAAA
ATAGTAGATGTAGATTTACAAGAAATAAAATTCTTTAAACTTAAAAGAGATGTTTTAGTTAATGGGAAAAAGTGCTTAGT
ATCAAGAACCGGTTATACTGGAGAAGATGGTTTTGAAATTTATTGTAAACCAGAGGATGCAAAAGGACTATGGCATGCTA
TATTAAATGCAGGAAAAGAAGAAGGTGCACAGCCAATAGGATTAGGTGCTAGAGATACTTTAAGATTTGAAGCTAGTCTT
CTTTTATATGGAAATGAAATGGATGAAACAATTACACCATTAGAAGTAGGTATGGGATTCTTTGCAAAATTAAAAATTGA
AGAAGATTTTATAGGAAAAGATGCTTTAATAAAACAAAAAGCAGAAGGCGTAACTAGAAAATTGGTAGGATTTGAACTTT
TAGATAAGGGAATTCCGAGGCATGGTTATGAGGTTATTAAAGATGGAAAAGTTATAGGACATGTTACAACTGGATATAAA
TCTCCTACATTAAACAAAGCAATTGGATTAGCTTTAGTAGAAGAACAATATTCTAAAATAGGAACAGAGTTTAATATAAA
AGTTAGAAAAAAAGAATTAAAAGCTGTTGCTATAGATAAGAGATTCTATACTAAAAAAACAAAAACCAAATAA

Upstream 100 bases:

>100_bases
AACTTTACAACTTGGTTTTACACTAAATCATTTTCATAATTTTATTAAAACATATATATAAATATTTATGGGTAAATAAT
TTTAATTGGGGGTAAAACAC

Downstream 100 bases:

>100_bases
TATATTAACGGTTAATTTGTAGATTATTTTTTAATATATATAAATTCACACTAATGTTTAGTTTTCAGGCTAAAATGTCA
TTGGTAATCAATATATAATA

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKDLKVTPLRGVYEEYGGKIVDFAGYELPTQFKGFLHEHHTVREKAGLFDVSHMGEAMVTGKDAGKFIQYLMTNDINVLK
DNEVLYTFMCNEDGGVIDDLLVYKFAEDEFFLVINASNKDKDVKWIMDHKGDFDVEIVDVSDSIAQLAFQGPLAEEILQK
IVDVDLQEIKFFKLKRDVLVNGKKCLVSRTGYTGEDGFEIYCKPEDAKGLWHAILNAGKEEGAQPIGLGARDTLRFEASL
LLYGNEMDETITPLEVGMGFFAKLKIEEDFIGKDALIKQKAEGVTRKLVGFELLDKGIPRHGYEVIKDGKVIGHVTTGYK
SPTLNKAIGLALVEEQYSKIGTEFNIKVRKKELKAVAIDKRFYTKKTKTK

Sequences:

>Translated_370_residues
MKDLKVTPLRGVYEEYGGKIVDFAGYELPTQFKGFLHEHHTVREKAGLFDVSHMGEAMVTGKDAGKFIQYLMTNDINVLK
DNEVLYTFMCNEDGGVIDDLLVYKFAEDEFFLVINASNKDKDVKWIMDHKGDFDVEIVDVSDSIAQLAFQGPLAEEILQK
IVDVDLQEIKFFKLKRDVLVNGKKCLVSRTGYTGEDGFEIYCKPEDAKGLWHAILNAGKEEGAQPIGLGARDTLRFEASL
LLYGNEMDETITPLEVGMGFFAKLKIEEDFIGKDALIKQKAEGVTRKLVGFELLDKGIPRHGYEVIKDGKVIGHVTTGYK
SPTLNKAIGLALVEEQYSKIGTEFNIKVRKKELKAVAIDKRFYTKKTKTK
>Mature_370_residues
MKDLKVTPLRGVYEEYGGKIVDFAGYELPTQFKGFLHEHHTVREKAGLFDVSHMGEAMVTGKDAGKFIQYLMTNDINVLK
DNEVLYTFMCNEDGGVIDDLLVYKFAEDEFFLVINASNKDKDVKWIMDHKGDFDVEIVDVSDSIAQLAFQGPLAEEILQK
IVDVDLQEIKFFKLKRDVLVNGKKCLVSRTGYTGEDGFEIYCKPEDAKGLWHAILNAGKEEGAQPIGLGARDTLRFEASL
LLYGNEMDETITPLEVGMGFFAKLKIEEDFIGKDALIKQKAEGVTRKLVGFELLDKGIPRHGYEVIKDGKVIGHVTTGYK
SPTLNKAIGLALVEEQYSKIGTEFNIKVRKKELKAVAIDKRFYTKKTKTK

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=376, Percent_Identity=35.3723404255319, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI257796258, Length=353, Percent_Identity=35.4107648725212, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI24797151, Length=345, Percent_Identity=32.463768115942, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI257796254, Length=373, Percent_Identity=32.7077747989276, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI257796256, Length=320, Percent_Identity=34.375, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI197927446, Length=325, Percent_Identity=28, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI21361378, Length=325, Percent_Identity=28, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI194306651, Length=376, Percent_Identity=25.531914893617, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1789272, Length=368, Percent_Identity=39.945652173913, Blast_Score=242, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17560118, Length=354, Percent_Identity=38.135593220339, Blast_Score=220, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI32563613, Length=327, Percent_Identity=26.9113149847095, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71994045, Length=331, Percent_Identity=26.8882175226586, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71994052, Length=331, Percent_Identity=26.8882175226586, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320222, Length=371, Percent_Identity=35.0404312668464, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI20129441, Length=360, Percent_Identity=34.4444444444444, Blast_Score=201, Evalue=7e-52,
Organism=Drosophila melanogaster, GI28571104, Length=286, Percent_Identity=29.020979020979, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI20130091, Length=316, Percent_Identity=24.3670886075949, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_CLOB1 (A7FRV3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001383080.1
- ProteinModelPortal:   A7FRV3
- SMR:   A7FRV3
- STRING:   A7FRV3
- GeneID:   5397737
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0735
- eggNOG:   COG0404
- HOGENOM:   HBG299834
- OMA:   LMTNDIN
- ProtClustDB:   PRK00389
- BioCyc:   CBOT441770:CLB_0735-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 41629; Mature: 41629

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLKVTPLRGVYEEYGGKIVDFAGYELPTQFKGFLHEHHTVREKAGLFDVSHMGEAMVT
CCCCCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEE
GKDAGKFIQYLMTNDINVLKDNEVLYTFMCNEDGGVIDDLLVYKFAEDEFFLVINASNKD
CCCHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCHHHHHEEEECCCCEEEEEECCCCC
KDVKWIMDHKGDFDVEIVDVSDSIAQLAFQGPLAEEILQKIVDVDLQEIKFFKLKRDVLV
CCEEEEECCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHC
NGKKCLVSRTGYTGEDGFEIYCKPEDAKGLWHAILNAGKEEGAQPIGLGARDTLRFEASL
CCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEEEE
LLYGNEMDETITPLEVGMGFFAKLKIEEDFIGKDALIKQKAEGVTRKLVGFELLDKGIPR
EEECCCCCCCCCHHHHCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
HGYEVIKDGKVIGHVTTGYKSPTLNKAIGLALVEEQYSKIGTEFNIKVRKKELKAVAIDK
CCHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEECH
RFYTKKTKTK
HHHCCCCCCC
>Mature Secondary Structure
MKDLKVTPLRGVYEEYGGKIVDFAGYELPTQFKGFLHEHHTVREKAGLFDVSHMGEAMVT
CCCCCCCCCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEE
GKDAGKFIQYLMTNDINVLKDNEVLYTFMCNEDGGVIDDLLVYKFAEDEFFLVINASNKD
CCCHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCHHHHHEEEECCCCEEEEEECCCCC
KDVKWIMDHKGDFDVEIVDVSDSIAQLAFQGPLAEEILQKIVDVDLQEIKFFKLKRDVLV
CCEEEEECCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHC
NGKKCLVSRTGYTGEDGFEIYCKPEDAKGLWHAILNAGKEEGAQPIGLGARDTLRFEASL
CCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHCCHHHCCCCCCCCCCCEEEEEEEE
LLYGNEMDETITPLEVGMGFFAKLKIEEDFIGKDALIKQKAEGVTRKLVGFELLDKGIPR
EEECCCCCCCCCHHHHCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
HGYEVIKDGKVIGHVTTGYKSPTLNKAIGLALVEEQYSKIGTEFNIKVRKKELKAVAIDK
CCHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEECH
RFYTKKTKTK
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA