| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is amiA [C]
Identifier: 153936707
GI number: 153936707
Start: 3174138
End: 3177041
Strand: Reverse
Name: amiA [C]
Synonym: CLC_3013
Alternate gene names: 153936707
Gene position: 3177041-3174138 (Counterclockwise)
Preceding gene: 153937646
Following gene: 153936078
Centisome position: 84.48
GC content: 28.0
Gene sequence:
>2904_bases ATGAGGAATAAAAAACATTATTTAGTACCTAAGATTATTTCAATATTTTTTATGTTATTTTTATTTTTTATAAAAGTACA AGCTGTATCGAACTTAAGTATTAATTTGAAAACACCAACAGATCAAAGCATAATAAATAGTGATGAGTTAAAAATTGATG GAGAAATAGAATCTGCTTTAAAATTAAAATCAATATATATATATATTGATAATGAATTCATAGGGAAAGCTAACTTAAAT AAATTAGAATTTAAGGAAAATACATACAGAACTACATTAAAATATACTAAAGATATTTCTTCTCTGAAAAATGGAATACA TAATTTAAAAATTTTAGCTATAGATGAACAGAATAACAAAGAAGAAAAAGAAATTAGTATGAATATAAAAAGGAACCAAG AAAAATTAGAAACTGATAAAAATATTATAGAGAATAATTTGGTAGATGCTACTGTAAAGATGGATAGTGTAGAAAAAGCA TTAACCACTGTATCTAATGAAGCTAAGCTAGAAAAAGTAGAAGTTTCATACAATGGTAATGTGATAACCAATGGAGAAAT AGGAGTTGGAAAAAGTTACGTTATAAAAGGATATGGAAATTCTGAAAATGGGGTACTGTATCAATTTTGGGTAAAAGATC TATCCACAAATAGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAG TATCTGATAGGAATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAGAATTATACAGTAAC TGTAAGTAAAGCTAAATTAGAAAAAGTGGAAGTTTCATACGATGGTAATGTAATAACTAATGGAGAAATAGGAGTTGGAA AAAGCTACGTTATAAAAGGATATGGAAATTCTGAAAATGGAGTATTGTATCAATTTTGGGTAAAGGATTTATCTACAAAC AGTTGGACAATGATAAGAGATTATGGAGAAACAAATAGTTTTAATTACACACCAACAAAGGCTGGAAAGTATCTGATAGG AATACATGTAAAAGATAAGTATAGTAAAGAAAATTTAGATGATTTTATATATGAGAATTATACAGTAACTGTAAGTAAAG CTAAATTAGAAAAAGTGGAAGTTTCATACGATGGTAATGTAATAACTAATGGAGAAATAGGAGTTGGAAAAAGCTACGTT ATAAAAGGATATGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATCTATCCACAAATAGTTGGACAAT GATAAGAGATTATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGCTGGAAAGTATCTGATAGGAATACATGTAA AAGATAAGTATAGTAAAGAGAATTTAGATGATTTTATATATGAGAATTATGATGTGTCTATAAGTAAAGCTAAATTAGAA AAAGTGGAAGTTTCATACAATGGTAATGTAATAACTGATGGAGAAATAGGAGTTGGAAAAAGCTACGTTATAAAAGGATA TGGAAATTCTGAAAATGGAGTACTGTATCAATTTTGGGTAAAGGATCTATCTACAAATAGTTGGACAATGATAAGAGATT ATGGAGAAACAAATAGTTTTAATTATACACCAGCAAAGGCTGGAAAGTATCTGATAGGAATACATGTAAAAGATAAGTAT AGTAAAGAGAATTTAGATGATTTTATATATGAGAATTATGATGTGTCTATAAGTAAAGCTAAGTTGGAAAAAGTAGAAGT CTCATACAATGGGAATGCAATAACCAATAGTGAAATAGAGACTGGAAAAAATTATACTATAAAAGGATATGGAAATTCTA AAAATGAGATACTGTATCAATTCTGGGTAAAGGATTTATATACAAATAGTTGGACAATGATAAAAGATTATGGAGAAGAG AATAGTATAAATTGGAAACCTACTATTGGTGGTAAATATTTAATAGGAATACATGTAAAGGATAAATATAGTGCTGATAC ATTAGATGATCACATATATGAGAATTATACAATATTGAGTGATAATGCAAGGCTAGAAAAGGTAGAAGTTAAATTAGATG GAAACCTAATAAGTAATAATTTAAATGTAGGAAAGACATATACAATAGAAGGAAGTGCTACATCTAAAAATGGAGTGCTA TATCAATTTTGGGTAAAAGACTTGTCTATAAATAGCTGGGCAATGTTAAGGGATTATGGAGAATCAAATAATATAACATA TACACCAACAAAGAGTGGACAATATTTAATAGGGGTACATGTAAAAGATAAGAATAGCAAGGAAAGCTTAGATGATTTTA AATACAATCAATATAGCATTATAGGTTATAATAAAACAGTAATTGTTGATGCAGGACATGGAGGATATGATAGTGGCGCT GTAGGACCAACCGGTATTAAAGAAAAGGATGTCACTTTAAAAGTAGCTTTAAAGTTAGGAAAAATTTTAGAAAATAGTGG GGTAAATGTTATTTATACTAGAACTAGTGATAATGTATCTTGGCCTTCAAATGAATCACAAGATTTAGCAGCTAGAGTGG CCATTGCAAATAACAATAATACAGATTTATATGTAAGTATACATGCTAATAGTTTTAATGGATCAGCGAATGGAACAGAA ACATATTACTATGATGGGAGTGCAAAAGGAAAAGAAACAGCAGAAAAAGTTCAAAAGGAACTTGTAAATTCTATTGGCTT ATATGATAGAGGCGTTAAAACAGCAGGATATTATGTTTTAAAAAATACTATAAGCCCATCTATATTGGTTGAATTAGGAT TCATAGATAATAGAAATGAAGAAATTCTTTTAAATTCTGATTGGTTTCAACAAAAGTGTGCCGAAGCTATTGCTAAAGGA ATATTATCAACTAGCTTTATGTAA
Upstream 100 bases:
>100_bases TAATAAAAATAATAAATATTTTTTTACTTTAAGTATAAAAATATTTATAGGAATTACGATAAATAAACTTATAAATATAA TTATTGAAAGGATGAGGGAG
Downstream 100 bases:
>100_bases CCTAATGAATATAAAAAATAATAAATCAATAAAATAATTATAAAATTGCATATTAAGCTCTAATTATGCTAGTCTTAAAA TAAATAAAAAACCATTAATT
Product: putative N-acetylmuramoyl-L-alanine amidase
Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]
Alternate protein names: NA
Number of amino acids: Translated: 967; Mature: 967
Protein sequence:
>967_residues MRNKKHYLVPKIISIFFMLFLFFIKVQAVSNLSINLKTPTDQSIINSDELKIDGEIESALKLKSIYIYIDNEFIGKANLN KLEFKENTYRTTLKYTKDISSLKNGIHNLKILAIDEQNNKEEKEISMNIKRNQEKLETDKNIIENNLVDATVKMDSVEKA LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKY SKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIETGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEE NSINWKPTIGGKYLIGIHVKDKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILSTSFM
Sequences:
>Translated_967_residues MRNKKHYLVPKIISIFFMLFLFFIKVQAVSNLSINLKTPTDQSIINSDELKIDGEIESALKLKSIYIYIDNEFIGKANLN KLEFKENTYRTTLKYTKDISSLKNGIHNLKILAIDEQNNKEEKEISMNIKRNQEKLETDKNIIENNLVDATVKMDSVEKA LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKY SKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIETGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEE NSINWKPTIGGKYLIGIHVKDKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILSTSFM >Mature_967_residues MRNKKHYLVPKIISIFFMLFLFFIKVQAVSNLSINLKTPTDQSIINSDELKIDGEIESALKLKSIYIYIDNEFIGKANLN KLEFKENTYRTTLKYTKDISSLKNGIHNLKILAIDEQNNKEEKEISMNIKRNQEKLETDKNIIENNLVDATVKMDSVEKA LTTVSNEAKLEKVEVSYNGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTN SWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYV IKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKY SKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIETGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEE NSINWKPTIGGKYLIGIHVKDKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSIIGYNKTVIVDAGHGGYDSGA VGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVSWPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTE TYYYDGSAKGKETAEKVQKELVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG ILSTSFM
Specific function: Unknown
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Secreted, cell wall, S-layer (Potential) [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 3 SLH (S-layer homology) domains [H]
Homologues:
Organism=Escherichia coli, GI1788776, Length=228, Percent_Identity=32.0175438596491, Blast_Score=99, Evalue=9e-22, Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=31.8181818181818, Blast_Score=97, Evalue=3e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002508 - InterPro: IPR001119 [H]
Pfam domain/function: PF01520 Amidase_3; PF00395 SLH [H]
EC number: 3.5.1.28 [C]
Molecular weight: Translated: 109259; Mature: 109259
Theoretical pI: Translated: 5.95; Mature: 5.95
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 1.1 %Met (Translated Protein) 1.2 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 1.1 %Met (Mature Protein) 1.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRNKKHYLVPKIISIFFMLFLFFIKVQAVSNLSINLKTPTDQSIINSDELKIDGEIESAL CCCCCCCHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCHHCCCCCEEECCCHHCCE KLKSIYIYIDNEFIGKANLNKLEFKENTYRTTLKYTKDISSLKNGIHNLKILAIDEQNNK EEEEEEEEECCEEECCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCEEEEEEEECCCCC EEKEISMNIKRNQEKLETDKNIIENNLVDATVKMDSVEKALTTVSNEAKLEKVEVSYNGN CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCEEEEEEEEECCC VITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCC YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH DFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE CCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSF EEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCC NYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIE CCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEEEEEEEECCCEEECCCCC TGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEENSINWKPTIGGKYLIGIHVK CCCCEEEEECCCCCCCEEEEEEHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEEEEEE DKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL CCCCCCCHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCCEE YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSI EEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCEEE IGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVS EECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCEEEEEECCCCCC WPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKE CCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHH LVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG HHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHH ILSTSFM HHHHCCC >Mature Secondary Structure MRNKKHYLVPKIISIFFMLFLFFIKVQAVSNLSINLKTPTDQSIINSDELKIDGEIESAL CCCCCCCHHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCCCHHCCCCCEEECCCHHCCE KLKSIYIYIDNEFIGKANLNKLEFKENTYRTTLKYTKDISSLKNGIHNLKILAIDEQNNK EEEEEEEEECCEEECCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCEEEEEEEECCCCC EEKEISMNIKRNQEKLETDKNIIENNLVDATVKMDSVEKALTTVSNEAKLEKVEVSYNGN CCCEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHCCCCCEEEEEEEEECCC VITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGK EEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCC YLIGIHVKDKYSKENLDDFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKG EEEEEEEECCCCCCCCHHHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEE YGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSFNYTPTKAGKYLIGIHVKDKYSKENLD CCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCH DFIYENYTVTVSKAKLEKVEVSYDGNVITNGEIGVGKSYVIKGYGNSENGVLYQFWVKDL HHHHCCEEEEEEECEEEEEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECC STNSWTMIRDYGETNSFNYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLE CCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEE KVEVSYNGNVITDGEIGVGKSYVIKGYGNSENGVLYQFWVKDLSTNSWTMIRDYGETNSF EEEEEECCCEEECCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCC NYTPAKAGKYLIGIHVKDKYSKENLDDFIYENYDVSISKAKLEKVEVSYNGNAITNSEIE CCCHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCEEEEEEEEEEEEEEECCCEEECCCCC TGKNYTIKGYGNSKNEILYQFWVKDLYTNSWTMIKDYGEENSINWKPTIGGKYLIGIHVK CCCCEEEEECCCCCCCEEEEEEHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEEEEEE DKYSADTLDDHIYENYTILSDNARLEKVEVKLDGNLISNNLNVGKTYTIEGSATSKNGVL CCCCCCCHHHHHHCCEEEEECCCEEEEEEEEECCEEEECCCCCCEEEEEECCCCCCCCEE YQFWVKDLSINSWAMLRDYGESNNITYTPTKSGQYLIGVHVKDKNSKESLDDFKYNQYSI EEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHCCCCCEEE IGYNKTVIVDAGHGGYDSGAVGPTGIKEKDVTLKVALKLGKILENSGVNVIYTRTSDNVS EECCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCCEEEEEECCCCCC WPSNESQDLAARVAIANNNNTDLYVSIHANSFNGSANGTETYYYDGSAKGKETAEKVQKE CCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHH LVNSIGLYDRGVKTAGYYVLKNTISPSILVELGFIDNRNEEILLNSDWFQQKCAEAIAKG HHHHCCCCCCCCCCCCEEEEECCCCCEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHH ILSTSFM HHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]
Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]
General reaction: Carboxylic acid amide hydrolysis [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10475962; 12004073 [H]