| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is amiC [H]
Identifier: 153936078
GI number: 153936078
Start: 3171052
End: 3173943
Strand: Reverse
Name: amiC [H]
Synonym: CLC_3012
Alternate gene names: 153936078
Gene position: 3173943-3171052 (Counterclockwise)
Preceding gene: 153936707
Following gene: 153935280
Centisome position: 84.4
GC content: 29.7
Gene sequence:
>2892_bases ATGAATGGAAAAAAATTTTTACTAAAAGAAAGGAGGGGAAATATGTATATGGGGGAAATAGAAAATATAGTTAAAAAGAT TCCATTAATACTTTTAATTAGTATTATTGTAACTTTTGTACCTTCATTTAAAGTACATGCTAGTAGCACTACACATATAA TGGGACAACCTCAGTTAACACAGGAACAAGCATTAAATTATTTTAAGACCAGAAACAGTGAAAAAAGTGACCAAGCTACA AAAGAATTTATTTCCATAGTTTGGCAGGAAGCTAATTTAGAAGGAATAAGAGCTGATGTAGTTTTTATTCAAATAATGAA GGAAACAAATTTTTTGAAATTTACAGGGGATGTAAAAGAATGTCAAAATAATTTTGCAGGTATTGGTGCTACAGGTGGTG GTGTACCAGGTGCTTATTTTAAAGATACAAAAACAGGAGTAAGAGCCGTTGTTCAACATCTAAAAGCTTATTGTAGTACT GAGGGATTAAAAAATCCTTGTGTAGATCCACGATTTACATATGTTCAAAGAGGAATAAGTCCCTATGTTGAGTGGTTAGG TATAGGGGAAAATCCTAATTATCCAGATAAAGGATGGGCTGCGGATAATAATTATGGTAAGAGTATAGTTGAAATGATGC ACAGTGCAAAGTATCTGGCAAATGAAGGTGATTCGCAAGGAAATATAACTACTTCAAAGGCTACTATTAATAATTTAGAA GTAAGTTTAGATGGAAATAGTGTAGTAACTAATGAATTAGAACCGGGAAAAGCATATAACATAAAAGCTTATGGGAATAG TTCTAATGGAGTACTATATGAATATTGGATAAAAGACTTATCAATTAATTCATGGATAAAGCTAAGAGATTATAGTACAA CCAATGAAGTTAAATGGACACCAAATAAATCAGGGAAATATCTAATAGGAGTACATGTTAAAGATAGATATAGTAAGGAA AGATTAGATAATTTTAAGTATGTAGAATATAATGTAGCATCCCTTAAAAAAGCTACTATTAGTAGTTTAGAAGTAAGCCT AGATGGAAATAATGTAGTGAATAATGAATTACAACTAGGCAAGATATATAATATAAAAGCTTATGGCAGTAGCTCTAATG GAGTATTGTATGAATATTGGATAAAAGACTTATCAAAGAATTCATGGATGAAGTTAAGAGATTATAGTACAAGTACTCAA GTCACATGGACACCAAATAAATCAGGAAAGTATCTACTAGGGGTACATGTTAAAGATAGGTATAGTGAAGAAAGATTAGA TAACTTTAAGTATGTAGAATACAATGTAGCATCACCTAAAAAGGCTACTGTTGATAGCTTAGAAGTAAGCTTAGATGGAA ATAAGGTAGAAACTAATGAATTAGATCCAGGAAAAGCATATAACATAAAAGCTTATGGGAATAGTTCTAATGGTGTATTA TATGAATATTGGATAAAAGACTTATCGATTAATTCATGGATAAAGTTAAAAGATTATAGTACAAGTACTCAAGTTACATG GACACCGAATAAGTCAGGAAAATATTTAATAGGAGTGCACGTAAAAGATAAATATAGTACACAAAAATTAGATAATTTTA AATATGTAGAATATAATGTAGCATCACCTAAAAAAGCTACTGTCAATAGTTTAGAAGTAAGTCTAAATGAAGGCAAAGTA GTAAATAATGAACTACAAGCAGGTGAAATATATAATATAAAAGCTTATGGTAGTAGTTCTAATGGAGTATTATATGAATA CTGGATAAAAGACCTATCAATTAATTCATGGATAAAGTTAAAAGATTATAGTACAAGTACTCAAGTTACATGGACACCAA ATAAGTCAGGAAAATATCTGATAGGGGTACACGTAAAAGATAAATATAGTGCACAGAAATTAGATAATTTTAAGTATGTA GAATATAATGTGAAACTATCTAAAAAAGCAGTTATTAGTAACTTGGAAGTAAGCCTAAATGGAAATATAGTAGCAAACAA TCAATTAAATTCAGGAAAAACATATAGTATAAAAGCTTATGCCGAAAGTCTTAATGGAGTATTGTATGAATATTGGATAA AAGATTTATCAAGTAATTCATGGATAAAGTTAAAAGATTATAGTACAAGTGCTCAAATCACATGGACACCAAATAAAGCA GGAAAATATCTAATAGGAGTACATGTAAAAGATAAATATAGTAATGAAAGATTAGACAATTATAAATATGTAGAATATAG TGTTCAAGGTGGTCTAATAAAAACTATAGTTCTAGATGCAGGTCATGGAGGCAAGGACTCAGGTGCAGTATCTTCTAGGG CAACTGGAAATATACACGAAGCAGATATAGTTCAAAAAATTACCATCAAGTTGGGAAACCTGCTAAAAAAAGCAGGATAT AATGTTATTTATACTAGAGATAAGGTAGATAATTATAATTATACCTCAGTAACACAAAATTTAGAAGATAGAATAAATGT GGCTAATAGTATAAATGCTGATTTGTTTATGAGTATACATGCTGATTCTTTTGATATACCATCAGCTAATGGATATAGCG CTCATTATAGTAGTTATAGACCTAAGCTAGATAATTCAGGTGTATATATAGAGGATGATGTGTATTATGATAGAACTCCT TGTGATGCAGCATTAAAGAGTAAAGTTTTATCACAACTTATAGTAAATGAGATGTCTTCTTTAGGAGGATCAAATAGAGG AATAAGTGATCATAATTTATATGTTACAAAAAATGCTCTAATGCCTTCTGTACTTGTAGAATGTGGATTTGTTTCTAATG ATGCAGAAGTTAGAAAGTTAAATGGTGACACTTATCAAAATCAAATAGCTCAAAAACTTTACAATGCAGTAACAAAATTG TTTAGTATGTAG
Upstream 100 bases:
>100_bases TTAATTACATATAATTGATGGTTTTTTATATTTTAGATATTATATAAATAAAATTATAAATAAAACATATGATATAATAA TTTACAAAGGAGGTGAATTT
Downstream 100 bases:
>100_bases TTTAGAAAACCGTTGATATATTTCTTATCAACGGTTTTATTAATTTATTTATAACTTATAAATGATATTTTCTATTTATT GTATATATGATAAAATAAAG
Product: putative N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 963; Mature: 963
Protein sequence:
>963_residues MNGKKFLLKERRGNMYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSEKSDQAT KEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCST EGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLE VSLDGNSVVTNELEPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSKE RLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQ VTWTPNKSGKYLLGVHVKDRYSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVL YEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVSLNEGKV VNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYV EYNVKLSKKAVISNLEVSLNGNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKA GKYLIGVHVKDKYSNERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNLLKKAGY NVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTP CDAALKSKVLSQLIVNEMSSLGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKL FSM
Sequences:
>Translated_963_residues MNGKKFLLKERRGNMYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSEKSDQAT KEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCST EGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLE VSLDGNSVVTNELEPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSKE RLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQ VTWTPNKSGKYLLGVHVKDRYSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVL YEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVSLNEGKV VNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYV EYNVKLSKKAVISNLEVSLNGNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKA GKYLIGVHVKDKYSNERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNLLKKAGY NVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTP CDAALKSKVLSQLIVNEMSSLGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKL FSM >Mature_963_residues MNGKKFLLKERRGNMYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLTQEQALNYFKTRNSEKSDQAT KEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKECQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCST EGLKNPCVDPRFTYVQRGISPYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLE VSLDGNSVVTNELEPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWTPNKSGKYLIGVHVKDRYSKE RLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLGKIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQ VTWTPNKSGKYLLGVHVKDRYSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVL YEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNVASPKKATVNSLEVSLNEGKV VNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYV EYNVKLSKKAVISNLEVSLNGNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKA GKYLIGVHVKDKYSNERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHEADIVQKITIKLGNLLKKAGY NVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIHADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTP CDAALKSKVLSQLIVNEMSSLGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKL FSM
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI87082163, Length=229, Percent_Identity=30.5676855895196, Blast_Score=85, Evalue=2e-17, Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=27.6595744680851, Blast_Score=75, Evalue=2e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR021731 - InterPro: IPR002508 [H]
Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 108256; Mature: 108256
Theoretical pI: Translated: 9.25; Mature: 9.25
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.2 %Met (Translated Protein) 1.8 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.2 %Met (Mature Protein) 1.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNGKKFLLKERRGNMYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLT CCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCC QEQALNYFKTRNSEKSDQATKEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKE HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEECCHHH CQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTYVQRGIS HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC PYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLE HHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEEEEE VSLDGNSVVTNELEPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWT EEECCCEEEECCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEEC PNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLG CCCCCCEEEEEEECCHHCHHHHCCCEEEEEEHHHHHHHEEEEEEEEECCCCEECCCEEEC KIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLGVHVKDR EEEEEEEECCCCCCEEEEEEHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEHHC YSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVL CCHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCEEEEEEECCCCCCEE YEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNV EEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEEECCCCHHHCCCEEEEEEEC ASPKKATVNSLEVSLNEGKVVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKL CCCCCCEEEEEEEEECCCEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEEE KDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISNLEVSLN EECCCCEEEEECCCCCCCEEEEEEECCCCCHHHCCCEEEEEEEEEECCHHHEEEEEEEEC GNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKA CCEEECCCCCCCCEEEEEEHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCC GKYLIGVHVKDKYSNERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHE CCEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCH ADIVQKITIKLGNLLKKAGYNVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIH HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHCHHHHHHHHCCCCCEEEEEEE ADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLIVNEMSS CCCCCCCCCCCCCCHHCCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHH LGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKL CCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH FSM HCC >Mature Secondary Structure MNGKKFLLKERRGNMYMGEIENIVKKIPLILLISIIVTFVPSFKVHASSTTHIMGQPQLT CCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCC QEQALNYFKTRNSEKSDQATKEFISIVWQEANLEGIRADVVFIQIMKETNFLKFTGDVKE HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEEECCHHH CQNNFAGIGATGGGVPGAYFKDTKTGVRAVVQHLKAYCSTEGLKNPCVDPRFTYVQRGIS HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC PYVEWLGIGENPNYPDKGWAADNNYGKSIVEMMHSAKYLANEGDSQGNITTSKATINNLE HHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECEEEEEEEE VSLDGNSVVTNELEPGKAYNIKAYGNSSNGVLYEYWIKDLSINSWIKLRDYSTTNEVKWT EEECCCEEEECCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCEEEEEECCCCCCEEEC PNKSGKYLIGVHVKDRYSKERLDNFKYVEYNVASLKKATISSLEVSLDGNNVVNNELQLG CCCCCCEEEEEEECCHHCHHHHCCCEEEEEEHHHHHHHEEEEEEEEECCCCEECCCEEEC KIYNIKAYGSSSNGVLYEYWIKDLSKNSWMKLRDYSTSTQVTWTPNKSGKYLLGVHVKDR EEEEEEEECCCCCCEEEEEEHHHCCCCCCEEEEECCCCCEEEECCCCCCCEEEEEEEHHC YSEERLDNFKYVEYNVASPKKATVDSLEVSLDGNKVETNELDPGKAYNIKAYGNSSNGVL CCHHHHCCEEEEEEECCCCCCCCCCEEEEEECCCEEECCCCCCCCEEEEEEECCCCCCEE YEYWIKDLSINSWIKLKDYSTSTQVTWTPNKSGKYLIGVHVKDKYSTQKLDNFKYVEYNV EEEEEECCCCCCEEEEEECCCCEEEEECCCCCCCEEEEEEEECCCCHHHCCCEEEEEEEC ASPKKATVNSLEVSLNEGKVVNNELQAGEIYNIKAYGSSSNGVLYEYWIKDLSINSWIKL CCCCCCEEEEEEEEECCCEEECCCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCEEEE KDYSTSTQVTWTPNKSGKYLIGVHVKDKYSAQKLDNFKYVEYNVKLSKKAVISNLEVSLN EECCCCEEEEECCCCCCCEEEEEEECCCCCHHHCCCEEEEEEEEEECCHHHEEEEEEEEC GNIVANNQLNSGKTYSIKAYAESLNGVLYEYWIKDLSSNSWIKLKDYSTSAQITWTPNKA CCEEECCCCCCCCEEEEEEHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCC GKYLIGVHVKDKYSNERLDNYKYVEYSVQGGLIKTIVLDAGHGGKDSGAVSSRATGNIHE CCEEEEEEEECCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCH ADIVQKITIKLGNLLKKAGYNVIYTRDKVDNYNYTSVTQNLEDRINVANSINADLFMSIH HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHCHHHHHHHHCCCCCEEEEEEE ADSFDIPSANGYSAHYSSYRPKLDNSGVYIEDDVYYDRTPCDAALKSKVLSQLIVNEMSS CCCCCCCCCCCCCCHHCCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHH LGGSNRGISDHNLYVTKNALMPSVLVECGFVSNDAEVRKLNGDTYQNQIAQKLYNAVTKL CCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH FSM HCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10710307 [H]