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The map label for this gene is thiH [H]

Identifier: 153936420

GI number: 153936420

Start: 3699203

End: 3700579

Strand: Direct

Name: thiH [H]

Synonym: CLC_3561

Alternate gene names: 153936420

Gene position: 3699203-3700579 (Clockwise)

Preceding gene: 153935870

Following gene: 153934957

Centisome position: 98.37

GC content: 28.69

Gene sequence:

>1377_bases
ATGTTTATAAATCATGATTATATAGAAAATTTATTAAAAGAGGCTGAAAATGCTACAAATGAAGATATAGAAAAGGTTTT
AGATAAAGCCTGTAGTAATGAGAAGTTAAGTCATAAAGATATAGCTATACTTCTCCAAATAAAAGACAAAAAATTATTAT
CAAAAATGTTTAAGATCGCTGGAGACATAAAAAAATCTATATACGGCAATAGAATTGTTTTATTTGCTCCTTTATATATT
AGTGACTATTGCGTTAATAATTGTGTATATTGTGGTTATAAAAACTGTAATAGCTTTGAAAGAAGAAAGCTAACCCAAGA
AGAAATTAGAACTGAAGTTAAAATTCTTGAAAAGATGGGACATAAAAGATTAGCTTTAGAAGCTGGGGAAGATCCTGTAA
ACTGTGATATAGATTATATATTAGAAAGTATAAAAACTATATATGATACTCAAAATGAAAATGGAAATATAAGAAGAATA
AATGTTAATATTGCAGCAACCACTGTTGAAAATTATAAAAAATTAAAAGCTGCTAATATAGGAACTTATATATTATTTCA
AGAAACTTACCATAAACCTACCTATGAAAAAATGCATCCCAATTGCTTAAAAGATAGCTATGAATATCATCTAACTGCAT
TTGATAGAGCAATGGAAGGTGGGATAGATGATGTAGGTGCTGGAGTTCTTTTTGGCTTATCTAATTATAAATTTGAAGTT
TTAGGATTAATGCTTCATAATCAACATCTAGAAGAAAAACATGGCGTAGGGTTCCACACTATATCAGTTCCTAGGCTAAA
AAAAGCCGAAGGAATGGATCTTAAGATGTTTCCGCATTTAGTAGATGATGAAACTTTTAAACAAATAGTAGCTATACTAA
GAATAGCTGTACCTTTCACTGGTATAATATTATCCACAAGAGAAAGCGCAGAAATGAGAAAAGAAGTGATTGAATATGGT
GTTTCTCAAGTAAGTGCTGGTTCTTGTGCTGGAGTTGGTGGCTACAAAGAAAGAGAAGAGGGAAAAAATACTAACCAATT
TATCATTGCAGATCATAGAAGTCATCTAGATGTTTTAAAAGAATTAATTGAGGAAGGTCATATTCCAAGTTATTGTACGG
GTTGTTATAGAAAAGGTAGAACTGGTGATAGATTTATGAGTCTTGCTAAATCTGGCAATATTCAGTATGTATGTCAACCT
AATGCCATAATGACTTTAATGGAATTTTTATTAGATTATGGTGATGAAGAACTAAAAGAAAAAGGTGAAAAATTAATAAA
GGAAGAGATAAATAAAATAAAAAGAGAAGATATTAAAAATATAGTTATTCAAAACATAGAAAAAATAAAAAATGGTGAAA
GAGATTTATTTTTTTAG

Upstream 100 bases:

>100_bases
AGCTGCAGAGTGCAGGAAATGCATAGAAAATAGAATTAATAATGCTGGTTTTTCTCTAGACTTAACTAGAGGAGATAATA
TTTTATGGAGGCGAATATAA

Downstream 100 bases:

>100_bases
GAGGATTTATGAATACTACACCTAATGCCAATAGAATTCATATAACTTTTATAGGTAAAACTAATTCTGGAAAGTCTTCT
TTAATGAATGCAATAATAGG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MFINHDYIENLLKEAENATNEDIEKVLDKACSNEKLSHKDIAILLQIKDKKLLSKMFKIAGDIKKSIYGNRIVLFAPLYI
SDYCVNNCVYCGYKNCNSFERRKLTQEEIRTEVKILEKMGHKRLALEAGEDPVNCDIDYILESIKTIYDTQNENGNIRRI
NVNIAATTVENYKKLKAANIGTYILFQETYHKPTYEKMHPNCLKDSYEYHLTAFDRAMEGGIDDVGAGVLFGLSNYKFEV
LGLMLHNQHLEEKHGVGFHTISVPRLKKAEGMDLKMFPHLVDDETFKQIVAILRIAVPFTGIILSTRESAEMRKEVIEYG
VSQVSAGSCAGVGGYKEREEGKNTNQFIIADHRSHLDVLKELIEEGHIPSYCTGCYRKGRTGDRFMSLAKSGNIQYVCQP
NAIMTLMEFLLDYGDEELKEKGEKLIKEEINKIKREDIKNIVIQNIEKIKNGERDLFF

Sequences:

>Translated_458_residues
MFINHDYIENLLKEAENATNEDIEKVLDKACSNEKLSHKDIAILLQIKDKKLLSKMFKIAGDIKKSIYGNRIVLFAPLYI
SDYCVNNCVYCGYKNCNSFERRKLTQEEIRTEVKILEKMGHKRLALEAGEDPVNCDIDYILESIKTIYDTQNENGNIRRI
NVNIAATTVENYKKLKAANIGTYILFQETYHKPTYEKMHPNCLKDSYEYHLTAFDRAMEGGIDDVGAGVLFGLSNYKFEV
LGLMLHNQHLEEKHGVGFHTISVPRLKKAEGMDLKMFPHLVDDETFKQIVAILRIAVPFTGIILSTRESAEMRKEVIEYG
VSQVSAGSCAGVGGYKEREEGKNTNQFIIADHRSHLDVLKELIEEGHIPSYCTGCYRKGRTGDRFMSLAKSGNIQYVCQP
NAIMTLMEFLLDYGDEELKEKGEKLIKEEINKIKREDIKNIVIQNIEKIKNGERDLFF
>Mature_458_residues
MFINHDYIENLLKEAENATNEDIEKVLDKACSNEKLSHKDIAILLQIKDKKLLSKMFKIAGDIKKSIYGNRIVLFAPLYI
SDYCVNNCVYCGYKNCNSFERRKLTQEEIRTEVKILEKMGHKRLALEAGEDPVNCDIDYILESIKTIYDTQNENGNIRRI
NVNIAATTVENYKKLKAANIGTYILFQETYHKPTYEKMHPNCLKDSYEYHLTAFDRAMEGGIDDVGAGVLFGLSNYKFEV
LGLMLHNQHLEEKHGVGFHTISVPRLKKAEGMDLKMFPHLVDDETFKQIVAILRIAVPFTGIILSTRESAEMRKEVIEYG
VSQVSAGSCAGVGGYKEREEGKNTNQFIIADHRSHLDVLKELIEEGHIPSYCTGCYRKGRTGDRFMSLAKSGNIQYVCQP
NAIMTLMEFLLDYGDEELKEKGEKLIKEEINKIKREDIKNIVIQNIEKIKNGERDLFF

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=369, Percent_Identity=27.9132791327913, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 52427; Mature: 52427

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFINHDYIENLLKEAENATNEDIEKVLDKACSNEKLSHKDIAILLQIKDKKLLSKMFKIA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHH
GDIKKSIYGNRIVLFAPLYISDYCVNNCVYCGYKNCNSFERRKLTQEEIRTEVKILEKMG
HHHHHHHCCCEEEEEECHHHHHHHHHCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHHCC
HKRLALEAGEDPVNCDIDYILESIKTIYDTQNENGNIRRINVNIAATTVENYKKLKAANI
CCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEHHHHHHHHHCCC
GTYILFQETYHKPTYEKMHPNCLKDSYEYHLTAFDRAMEGGIDDVGAGVLFGLSNYKFEV
CEEEEEEHHCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHCCCCHHCCHHHHCCCCCCHHH
LGLMLHNQHLEEKHGVGFHTISVPRLKKAEGMDLKMFPHLVDDETFKQIVAILRIAVPFT
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
GIILSTRESAEMRKEVIEYGVSQVSAGSCAGVGGYKEREEGKNTNQFIIADHRSHLDVLK
HEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHHHH
ELIEEGHIPSYCTGCYRKGRTGDRFMSLAKSGNIQYVCQPNAIMTLMEFLLDYGDEELKE
HHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
KGEKLIKEEINKIKREDIKNIVIQNIEKIKNGERDLFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFINHDYIENLLKEAENATNEDIEKVLDKACSNEKLSHKDIAILLQIKDKKLLSKMFKIA
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHH
GDIKKSIYGNRIVLFAPLYISDYCVNNCVYCGYKNCNSFERRKLTQEEIRTEVKILEKMG
HHHHHHHCCCEEEEEECHHHHHHHHHCCEEECCCCCCCHHHHCCCHHHHHHHHHHHHHCC
HKRLALEAGEDPVNCDIDYILESIKTIYDTQNENGNIRRINVNIAATTVENYKKLKAANI
CCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEHHHHHHHHHCCC
GTYILFQETYHKPTYEKMHPNCLKDSYEYHLTAFDRAMEGGIDDVGAGVLFGLSNYKFEV
CEEEEEEHHCCCCCHHHCCCHHHHCCHHHHHHHHHHHHHCCCCHHCCHHHHCCCCCCHHH
LGLMLHNQHLEEKHGVGFHTISVPRLKKAEGMDLKMFPHLVDDETFKQIVAILRIAVPFT
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
GIILSTRESAEMRKEVIEYGVSQVSAGSCAGVGGYKEREEGKNTNQFIIADHRSHLDVLK
HEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEECCHHHHHHHH
ELIEEGHIPSYCTGCYRKGRTGDRFMSLAKSGNIQYVCQPNAIMTLMEFLLDYGDEELKE
HHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
KGEKLIKEEINKIKREDIKNIVIQNIEKIKNGERDLFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]