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The map label for this gene is greA-1 [H]

Identifier: 153936284

GI number: 153936284

Start: 748949

End: 749413

Strand: Direct

Name: greA-1 [H]

Synonym: CLC_0730

Alternate gene names: 153936284

Gene position: 748949-749413 (Clockwise)

Preceding gene: 153935524

Following gene: 153937827

Centisome position: 19.92

GC content: 29.03

Gene sequence:

>465_bases
ATGAAAAACATATTGACAGAAGAAAATATGAATAAATTAAAAGAAGAATTAGAATATAGAATGACTAAAAAAAGAGCTGA
AATAGCAAAGGAAAAATTAGAAGCAGCTGCTCATGGTGATAGGTCAGAAAATGCAGAATATAAAGAAGCCTGTGCCAATT
ATAGAGAAAATGACAATAGAATTCAATATTTATTAACTATGATTTCAACTGCAAGTGTAATAGATGAGAAAAATCAGAAT
AAGTCAGTACTAGGAGTTAATAGTAAATGTAAAATTAAATTTATAGAAGATGAATTTGAAACAAATGTATCACTAGTAAC
TACCATGGATGCAGATCCAGAAAAGATGCTTATAAGTGTAGAATCAGATTTAGGAAAAGCATTAATGGGAAAAAAAGTTG
GAGATGTGGCTGAAGTGGATGCGCCAGGTGAAAAATATACAGTAGAAGTATTAGAAATTATATAA

Upstream 100 bases:

>100_bases
CTACCAGAGTTATGTCCTAGTGTAGCATAATATTTCATTAGTATTTTACTATATAGTGTATAATATTAACATAACATATC
TTGGGGAGGATTATTTGTAC

Downstream 100 bases:

>100_bases
TTATTTGTTAACTTATCAGTTTTGTATTCTTTCTGATTTAGATAGTAGATAACAAATAATGTGAAATAGAGGGACTGTTC
TTAGATTCAAATAAAATTAA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNRIQYLLTMISTASVIDEKNQN
KSVLGVNSKCKIKFIEDEFETNVSLVTTMDADPEKMLISVESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII

Sequences:

>Translated_154_residues
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNRIQYLLTMISTASVIDEKNQN
KSVLGVNSKCKIKFIEDEFETNVSLVTTMDADPEKMLISVESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII
>Mature_154_residues
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNRIQYLLTMISTASVIDEKNQN
KSVLGVNSKCKIKFIEDEFETNVSLVTTMDADPEKMLISVESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=32.0261437908497, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=30.9210526315789, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17412; Mature: 17412

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCH
IQYLLTMISTASVIDEKNQNKSVLGVNSKCKIKFIEDEFETNVSLVTTMDADPEKMLISV
HHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCHHCCCEEEEEECCCCHHHEEEEH
ESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII
HHHHHHHHHCCCCCCHHCCCCCCCHHHHHEEECC
>Mature Secondary Structure
MKNILTEENMNKLKEELEYRMTKKRAEIAKEKLEAAAHGDRSENAEYKEACANYRENDNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCH
IQYLLTMISTASVIDEKNQNKSVLGVNSKCKIKFIEDEFETNVSLVTTMDADPEKMLISV
HHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCHHCCCEEEEEECCCCHHHEEEEH
ESDLGKALMGKKVGDVAEVDAPGEKYTVEVLEII
HHHHHHHHHCCCCCCHHCCCCCCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA