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The map label for this gene is dusC [H]

Identifier: 153935524

GI number: 153935524

Start: 747854

End: 748813

Strand: Direct

Name: dusC [H]

Synonym: CLC_0729

Alternate gene names: 153935524

Gene position: 747854-748813 (Clockwise)

Preceding gene: 153937350

Following gene: 153936284

Centisome position: 19.89

GC content: 25.73

Gene sequence:

>960_bases
ATGAAATATTATTTAGCGCCAATGGAAGGGATTACGGGGTACATATATAGAAGTTCTTATGAAAAGTTTTTTAATAATAT
TGATAAGTACTTTACACCTTTTATTGTTCCAAATAAAAGTACAAGCCTTAAAACTAAAGAATTAAGAGATATTTTGCCGG
AAAATAATAAAGGAATGAATATAGTACCTCAAATACTTACGAACGATTCAGAAGGTTTTATTATCACTGCTAGAAAGTTA
CAGCAATTAGGTTATAATGAGATTAACTTAAATTTAGGTTGTCCTGCAGGAACCGTTGTTTCAAAAAATAGAGGCTCAGG
ATTTTTAGCTAAAAGAGAAGAACTTGATATATTTTTAGATGAAATATTTAAAATAGATGATATGAAAATTTCTATAAAAA
CCAGGATAGGAAAAGATAGTCCAGAAGAATTTTATGAGTTAATAAAAATTTATAATAAATATCCTATAGAAGAATTAATT
ATTCATCCTAGAACGCAAAAAGATTTTTATGGGCATAAACCTGATTTAGATGTATTTAAAGATGCATTATCTTTAAGTAG
CAATCCTATATGTTATAATGGTGATATCTTTACTGTTAGTGATTATAACAGATTAATAAAAACTTTTCCTAAAGTAAAAA
CAATAATGCTAGGAAGAGGAATACTCGCAAATCCAGGGCTAATAAATGAGATAAAAAATAATACTACTATAGACAAAGAA
GTATTAAAAGAATTTCATGATGAAATTTTAAATAAATATATAGAATTATATAATGAAGATAGAAATGCACTATTTAGAAT
GAAAGAACTCTGGGGATATATGATTCACATATTTTCAAATAATAAAAAATATGCTAAAAAAATAAAGAAGGCACAAAAGT
TAAATTGTTATAATGCAGCTGTCTTAAGTTTATTTAGAGAGCAAGAAATTATAAAAGAGGTAGAATTATTTGGGAACTAA

Upstream 100 bases:

>100_bases
AGTTAGAAGTTTTTATAGCACATAAATTAATATTGAGTAGATAATAAAAAAGAAGATTGATATAATATACTTTTTAGAAT
ATTTATAAAGGAGTTATTAA

Downstream 100 bases:

>100_bases
TATTAAAATTTAAGTAAGCTAAACTTAAGGTTTCGCTACCAGAGTTATGTCCTAGTGTAGCATAATATTTCATTAGTATT
TTACTATATAGTGTATAATA

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKYYLAPMEGITGYIYRSSYEKFFNNIDKYFTPFIVPNKSTSLKTKELRDILPENNKGMNIVPQILTNDSEGFIITARKL
QQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLDEIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELI
IHPRTQKDFYGHKPDLDVFKDALSLSSNPICYNGDIFTVSDYNRLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIHIFSNNKKYAKKIKKAQKLNCYNAAVLSLFREQEIIKEVELFGN

Sequences:

>Translated_319_residues
MKYYLAPMEGITGYIYRSSYEKFFNNIDKYFTPFIVPNKSTSLKTKELRDILPENNKGMNIVPQILTNDSEGFIITARKL
QQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLDEIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELI
IHPRTQKDFYGHKPDLDVFKDALSLSSNPICYNGDIFTVSDYNRLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIHIFSNNKKYAKKIKKAQKLNCYNAAVLSLFREQEIIKEVELFGN
>Mature_319_residues
MKYYLAPMEGITGYIYRSSYEKFFNNIDKYFTPFIVPNKSTSLKTKELRDILPENNKGMNIVPQILTNDSEGFIITARKL
QQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLDEIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELI
IHPRTQKDFYGHKPDLDVFKDALSLSSNPICYNGDIFTVSDYNRLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIHIFSNNKKYAKKIKKAQKLNCYNAAVLSLFREQEIIKEVELFGN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788462, Length=297, Percent_Identity=25.5892255892256, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37157; Mature: 37157

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYLAPMEGITGYIYRSSYEKFFNNIDKYFTPFIVPNKSTSLKTKELRDILPENNKGMN
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCCE
IVPQILTNDSEGFIITARKLQQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLD
EHHHHHCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCEECHHHHHHHHH
EIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELIIHPRTQKDFYGHKPDLDVFK
HHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHH
DALSLSSNPICYNGDIFTVSDYNRLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
HHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHH
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIHIFSNNKKYAKKIKKAQKLNCYNAA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCHHHHH
VLSLFREQEIIKEVELFGN
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYYLAPMEGITGYIYRSSYEKFFNNIDKYFTPFIVPNKSTSLKTKELRDILPENNKGMN
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCCE
IVPQILTNDSEGFIITARKLQQLGYNEINLNLGCPAGTVVSKNRGSGFLAKREELDIFLD
EHHHHHCCCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCEECHHHHHHHHH
EIFKIDDMKISIKTRIGKDSPEEFYELIKIYNKYPIEELIIHPRTQKDFYGHKPDLDVFK
HHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCHHHHH
DALSLSSNPICYNGDIFTVSDYNRLIKTFPKVKTIMLGRGILANPGLINEIKNNTTIDKE
HHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHH
VLKEFHDEILNKYIELYNEDRNALFRMKELWGYMIHIFSNNKKYAKKIKKAQKLNCYNAA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCHHHHH
VLSLFREQEIIKEVELFGN
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]