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The map label for this gene is rnfC [H]

Identifier: 153936091

GI number: 153936091

Start: 422087

End: 423406

Strand: Direct

Name: rnfC [H]

Synonym: CLC_0426

Alternate gene names: 153936091

Gene position: 422087-423406 (Clockwise)

Preceding gene: 153935356

Following gene: 153937347

Centisome position: 11.22

GC content: 34.39

Gene sequence:

>1320_bases
ATGGAACTATTAACTTTTAAAAAAGGTGTACATCCACCACATGGAAAATACCTTACAGAGAAAAAGCCTATAGAAGAATA
TTTACCAAAAGGCGATTTAGTTTTTCCTATGTCCCAACATATAGGGGCACCTTGTAATCCTGTAGTTAAAAAGGGTGACA
GAGTATTAGTAGGTCAAGTTATAGGAGAGGCTACAGGTTTTGTATCAGCGCCTATACACAGTAGTGTTTCAGGAACTGTA
AAAAATCTTGCTCCAGTATTAACTGCAGCAGGAACAAAGGTAATGGCAGTTATTGTAGAAAATGATGGACAATATGAAGA
AATTGAAATGCCAAAGCCAAAAGACTACAATGAAATGACAAAAGAAGAAATAATTAAATTAATTCAACAAGCCGGTATAG
TTGGTATGGGTGGAGCTTGTTTCCCAACTCATGTAAAACTTTCACCACCACCAGACAAAAAAATAGACTCAATCATAGTA
AATGCAGCGGAATGTGAGCCATATTTAACTTGTGACCACAGACTTATGCTAGAAGAATCAGATAAAATAGTAGAAGGTTT
AAAAATAATATTAAAAATATTCCCAGAAGCTAAAGGCTATATAGGAATAGAAAATAATAAACCAGATGCTATAGAAGCTA
TGAAAAAAGCATCAGAAGGAATAGCAAATATAGAAGTTAAAGCTCTTAAAACTAAATATCCACAAGGAGCAGAAAAACAA
TTAATATACGCTATAAAAGGAAGAGAAGTTCAATCAGGAAAACTTCCAGCAGATGCAGGATGTGTTGTTCAAAACACTGC
AACAGTTAGAGAAATATATAATGCAGTAGTTTTAGGCAGACCATTGATAGAAAGAATTATAACAGTTACAGGTGAAGCTG
TTAAAGAACCTAAAAATATAAGAATGAAACTTGGAACAAACCTTAGAGAATTAGTAGAATTCTGTGGAGGATACAAAGAA
GATCCAGTTAAAGTTATATCCGGTGGTCCAATGATGGGCATGACAATACCAACATTAGATATACCAACAGTAAAAGGTTC
TTCAGGCTTATTAAGCTTAGCTGCAGCTCAAGCGGTATTACCACAAGCATCAAGCTGTATTAGATGTGGAAGATGTGTAG
AGGCATGTCCAATGAACTTGATACCATCTACATTAGATTCTTTATCTAACAGAGGTGAATTAGCTAGCTTTGAAGAATTA
CACGGTATAGATTGTATAGAATGTGGATGCTGTACTTTTGTATGTCCAGCTAAACGTCATTTAATCCAATCCATAAGAAC
AGCTAAACGTACAGTTATAGCTTCAAAAAGAAAAAAATAA

Upstream 100 bases:

>100_bases
TAATAATAAATTTACTGTTTTTATTCAATCCATATTTTAAGTAATAACATTGTGTATTTCATGATTTTTACCATGTATGC
ATAAAGATAGGAGGATTAGT

Downstream 100 bases:

>100_bases
ATTAAGTAAGGAGTGACTTTGGATGTCTGAATCAATGTATACATTATCTTCATCTCCACATATTCGTTCAAAAGATTCTG
TTCAATCAATAATGAGAGAT

Product: electron transport complex, RnfABCDGE type, C subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MELLTFKKGVHPPHGKYLTEKKPIEEYLPKGDLVFPMSQHIGAPCNPVVKKGDRVLVGQVIGEATGFVSAPIHSSVSGTV
KNLAPVLTAAGTKVMAVIVENDGQYEEIEMPKPKDYNEMTKEEIIKLIQQAGIVGMGGACFPTHVKLSPPPDKKIDSIIV
NAAECEPYLTCDHRLMLEESDKIVEGLKIILKIFPEAKGYIGIENNKPDAIEAMKKASEGIANIEVKALKTKYPQGAEKQ
LIYAIKGREVQSGKLPADAGCVVQNTATVREIYNAVVLGRPLIERIITVTGEAVKEPKNIRMKLGTNLRELVEFCGGYKE
DPVKVISGGPMMGMTIPTLDIPTVKGSSGLLSLAAAQAVLPQASSCIRCGRCVEACPMNLIPSTLDSLSNRGELASFEEL
HGIDCIECGCCTFVCPAKRHLIQSIRTAKRTVIASKRKK

Sequences:

>Translated_439_residues
MELLTFKKGVHPPHGKYLTEKKPIEEYLPKGDLVFPMSQHIGAPCNPVVKKGDRVLVGQVIGEATGFVSAPIHSSVSGTV
KNLAPVLTAAGTKVMAVIVENDGQYEEIEMPKPKDYNEMTKEEIIKLIQQAGIVGMGGACFPTHVKLSPPPDKKIDSIIV
NAAECEPYLTCDHRLMLEESDKIVEGLKIILKIFPEAKGYIGIENNKPDAIEAMKKASEGIANIEVKALKTKYPQGAEKQ
LIYAIKGREVQSGKLPADAGCVVQNTATVREIYNAVVLGRPLIERIITVTGEAVKEPKNIRMKLGTNLRELVEFCGGYKE
DPVKVISGGPMMGMTIPTLDIPTVKGSSGLLSLAAAQAVLPQASSCIRCGRCVEACPMNLIPSTLDSLSNRGELASFEEL
HGIDCIECGCCTFVCPAKRHLIQSIRTAKRTVIASKRKK
>Mature_439_residues
MELLTFKKGVHPPHGKYLTEKKPIEEYLPKGDLVFPMSQHIGAPCNPVVKKGDRVLVGQVIGEATGFVSAPIHSSVSGTV
KNLAPVLTAAGTKVMAVIVENDGQYEEIEMPKPKDYNEMTKEEIIKLIQQAGIVGMGGACFPTHVKLSPPPDKKIDSIIV
NAAECEPYLTCDHRLMLEESDKIVEGLKIILKIFPEAKGYIGIENNKPDAIEAMKKASEGIANIEVKALKTKYPQGAEKQ
LIYAIKGREVQSGKLPADAGCVVQNTATVREIYNAVVLGRPLIERIITVTGEAVKEPKNIRMKLGTNLRELVEFCGGYKE
DPVKVISGGPMMGMTIPTLDIPTVKGSSGLLSLAAAQAVLPQASSCIRCGRCVEACPMNLIPSTLDSLSNRGELASFEEL
HGIDCIECGCCTFVCPAKRHLIQSIRTAKRTVIASKRKK

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=441, Percent_Identity=39.2290249433107, Blast_Score=311, Evalue=6e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 47481; Mature: 47481

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLTFKKGVHPPHGKYLTEKKPIEEYLPKGDLVFPMSQHIGAPCNPVVKKGDRVLVGQV
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECHHHHCCCCCCHHHHCCCEEEEHHH
IGEATGFVSAPIHSSVSGTVKNLAPVLTAAGTKVMAVIVENDGQYEEIEMPKPKDYNEMT
HHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCCHHHHH
KEEIIKLIQQAGIVGMGGACFPTHVKLSPPPDKKIDSIIVNAAECEPYLTCDHRLMLEES
HHHHHHHHHHCCCEECCCCCCCCEEECCCCCCCCHHHHEEEHHCCCCEEECCCEEEECCH
DKIVEGLKIILKIFPEAKGYIGIENNKPDAIEAMKKASEGIANIEVKALKTKYPQGAEKQ
HHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCE
LIYAIKGREVQSGKLPADAGCVVQNTATVREIYNAVVLGRPLIERIITVTGEAVKEPKNI
EEEEEECCEECCCCCCCCCCEEEECHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHCCCCE
RMKLGTNLRELVEFCGGYKEDPVKVISGGPMMGMTIPTLDIPTVKGSSGLLSLAAAQAVL
EEECCCCHHHHHHHHCCCCCCCEEEEECCCEECEEECCCCCCCCCCCCCHHHHHHHHHHH
PQASSCIRCGRCVEACPMNLIPSTLDSLSNRGELASFEELHGIDCIECGCCTFVCPAKRH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHCCCCEEECCCHHEECHHHHH
LIQSIRTAKRTVIASKRKK
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELLTFKKGVHPPHGKYLTEKKPIEEYLPKGDLVFPMSQHIGAPCNPVVKKGDRVLVGQV
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECHHHHCCCCCCHHHHCCCEEEEHHH
IGEATGFVSAPIHSSVSGTVKNLAPVLTAAGTKVMAVIVENDGQYEEIEMPKPKDYNEMT
HHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCCCCCHHHHH
KEEIIKLIQQAGIVGMGGACFPTHVKLSPPPDKKIDSIIVNAAECEPYLTCDHRLMLEES
HHHHHHHHHHCCCEECCCCCCCCEEECCCCCCCCHHHHEEEHHCCCCEEECCCEEEECCH
DKIVEGLKIILKIFPEAKGYIGIENNKPDAIEAMKKASEGIANIEVKALKTKYPQGAEKQ
HHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCE
LIYAIKGREVQSGKLPADAGCVVQNTATVREIYNAVVLGRPLIERIITVTGEAVKEPKNI
EEEEEECCEECCCCCCCCCCEEEECHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHCCCCE
RMKLGTNLRELVEFCGGYKEDPVKVISGGPMMGMTIPTLDIPTVKGSSGLLSLAAAQAVL
EEECCCCHHHHHHHHCCCCCCCEEEEECCCEECEEECCCCCCCCCCCCCHHHHHHHHHHH
PQASSCIRCGRCVEACPMNLIPSTLDSLSNRGELASFEELHGIDCIECGCCTFVCPAKRH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHCCCCEEECCCHHEECHHHHH
LIQSIRTAKRTVIASKRKK
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]