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The map label for this gene is hemK [H]

Identifier: 153935975

GI number: 153935975

Start: 168303

End: 169154

Strand: Direct

Name: hemK [H]

Synonym: CLC_0186

Alternate gene names: 153935975

Gene position: 168303-169154 (Clockwise)

Preceding gene: 153937305

Following gene: 153936703

Centisome position: 4.48

GC content: 26.17

Gene sequence:

>852_bases
TTGATATTAAAAGATTTACTAATAGAAGGATATGGTGTTTTAAAAAAAGCATCTATAGGTAGCTATCAAATAGATGCTCA
ACTTTTATTAGGAAAAGTATTAAAAAAGGATAGATTGTTCATTTTGACTAATCCTGATTACCATATAAAAGAGGAAGAGA
AAGAAAAATATTTTCAATTAATAGACCTTAGAAAAAATAAAATGCCTATAAAATATATATTAGGTACAACTGAATTTATG
GGACTAAATTTTAACATAAAAAAGGGTGTATTAATACCAAGACCAGATACAGAAATATTAGTAGAAACGGTTTTAGAAGA
AATAAAGAATAAAAATTATAAGCAAATTTGTGATGTTTGTTGTGGTAGTGGTATTATAGGAATTACTATAGGATATACCT
TAAATAATACAGAAATAATTTGCTATGATATAGAAGATATTCCATACAATACTACTAAAGAAAATATACTCAAGCATAAT
CTTCAAGATAGAGTTAAAGCTTTAAAAAGTGATTTACTTACAGAGGCAATAAAAGAAAAAAGAAGGTTTGATCTTATAGT
ATCTAATCCACCATATATAAGAGAAGATGTTATTGAAACACTTATGGATGATGTTAAAAAATATGAACCCTTTGAAGCTT
TATGTGGTGGAAAAGATGGATTATTTTTTTATGAGGGTATAATAAAGCAAAGTTTAGAAGTGCTAAATGATGGGGGAACC
ATAGCTTTTGAAATAGGTTATGATCAAAAAATACAGGTTTCTCACATTTTAAATGAATATGGTTTTAAGGATGTATTATG
TATAAAAGACTTAGCTGGAAAAGACAGAGTTATAAAAGCTAGAAAGTATTAA

Upstream 100 bases:

>100_bases
AAACTAACAACTAAAGAACCTGACTTAAGGCAATTGGAAGTAGCTATAATGGCTTTAAAAGCAGCAGAAGGAATTGAAAG
TTCAATGGAGTGATGTTAAT

Downstream 100 bases:

>100_bases
AATTCACAGGTTAGTTGTAAATGCTTTATAAAGTATGATATAATATAAAATTGTGAAAATATATATACGGAGTGAGAAAT
AATGTTAGAAAGACTTAATT

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQLIDLRKNKMPIKYILGTTEFM
GLNFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVCCGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHN
LQDRVKALKSDLLTEAIKEKRRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY

Sequences:

>Translated_283_residues
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQLIDLRKNKMPIKYILGTTEFM
GLNFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVCCGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHN
LQDRVKALKSDLLTEAIKEKRRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY
>Mature_283_residues
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQLIDLRKNKMPIKYILGTTEFM
GLNFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVCCGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHN
LQDRVKALKSDLLTEAIKEKRRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=296, Percent_Identity=30.0675675675676, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1787463, Length=263, Percent_Identity=30.7984790874525, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082085, Length=213, Percent_Identity=28.169014084507, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324266, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582226, Length=253, Percent_Identity=27.6679841897233, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32517; Mature: 32517

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQL
CCHHHHHHHCCCHHEECCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
IDLRKNKMPIKYILGTTEFMGLNFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVC
HHHHCCCCCEEEEECCCEEEECCEEECCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
CGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHNLQDRVKALKSDLLTEAIKEK
HCCCEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY
EEEEECCCCEEHHHHHHHHHCCCCEEHHHHCCCCCCEEECCCC
>Mature Secondary Structure
MILKDLLIEGYGVLKKASIGSYQIDAQLLLGKVLKKDRLFILTNPDYHIKEEEKEKYFQL
CCHHHHHHHCCCHHEECCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
IDLRKNKMPIKYILGTTEFMGLNFNIKKGVLIPRPDTEILVETVLEEIKNKNYKQICDVC
HHHHCCCCCEEEEECCCEEEECCEEECCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
CGSGIIGITIGYTLNNTEIICYDIEDIPYNTTKENILKHNLQDRVKALKSDLLTEAIKEK
HCCCEEEEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRFDLIVSNPPYIREDVIETLMDDVKKYEPFEALCGGKDGLFFYEGIIKQSLEVLNDGGT
HHEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
IAFEIGYDQKIQVSHILNEYGFKDVLCIKDLAGKDRVIKARKY
EEEEECCCCEEHHHHHHHHHCCCCEEHHHHCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]