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The map label for this gene is citS [H]

Identifier: 153935941

GI number: 153935941

Start: 287746

End: 289368

Strand: Direct

Name: citS [H]

Synonym: CLC_0306

Alternate gene names: 153935941

Gene position: 287746-289368 (Clockwise)

Preceding gene: 153936944

Following gene: 153934977

Centisome position: 7.65

GC content: 29.33

Gene sequence:

>1623_bases
ATGAGAAAAGCAATGAAGCTACAAACGAAAATTACCTTATTAATTATTACAGTGGTTTTTCTTTCTATAGCTATAATTAT
TTCTTTTGTTGTTTCATGGATGACTGGAAACATTGAAAATAAAGCTAAAACTAATATTATGAATGTAGCGGAGATGGTAG
CGCATTCTACACAGATCGTAGATGCTTTAGAAGAAAAGGATCCTAACGGAAAGATTGGACCTTATATAAACATGCAGCTT
AAAAATTTACAACAGATTCAGTATATAATTGTAGCAGATAATGATGGAATTAGATATTCTCATCCAAATCCTAAAATGAT
AAAGAAGAAATTTGAAGGAGGGGATGAGTATAAGGTAGTCAAAGAAGGAGAAACGTATATATCTGAAGCTACAGGTACAT
TAGGAAAATCCATGAGGGCTTTTGCTCCAATATATGATAGAGAAAACAAAAAAGAGATAGGTTTTGTTTCCGTAGGCACA
CTTAATGAAAATATTGAAACGGCTAAGCATATAGCTATTTTATATATAATTTTAATTGGGTTTGGAGGGCTTATGGCAGG
AACAATAGGGGCAATATTACTTTCTAATAATATAAAAAATATTTTATTAGGACTAGAGCCAGAAGAAATAACAAATCTTT
ATAATGAGAAGATGGGAATTTTGGATACTATCCATGAAGGATTAGTAGCAGTTGATTATATGGGAAGAATTACCCTTATA
AATAATTCAGCTTTAAAAATATTAAATCTTCAACATAAAGTTGATAAGGATGAAGTCATAGGAAAAAATATAGAAAATAT
TATTGAAAATACAGGAATGATTAATATATTACAAACGGGAGAATCTGAATTTGAAAGAGAGCAGAAAATAAATAATACAA
CTATAATGACCAATAGAATTCCTATAATGAAAAGAGAAAAGATCGTGGGAGCTATTGCTAGTTTTAGGGATAAAACAGAA
GTGACTAGAATGGCAGAAGAACTTACAGGTGCGAAAAAAATGGCATGGTCTCTAAGAGCTCAAAATCATGAATTCATGAA
CAAACTTCATACTATTTCAGGACTTATACAGCTAGAAGAGTATGATGAAGCTCTACAGTTTATTTCAGATATGGCTAAGA
GTAGAAATAGTATAAGTAATATATTATCCCATAATATAAAAAATCCTTCTATATCTGCTTTATTGTTGTCAAAATATAAT
AAAGCTGAAGAGTGTAGAGTTAGTTTAAAAATAGATGAGAATTGTAAACTTACAAAATTACCACAGCATATGACCTCAGA
AGAAATTGTATCAATTATAGGTAATTTAATAGAGAATTCTTTAGATGAAGTTAAAAATGATGGTACTGGATTAATATATA
TAAAAATAGTAGAAAATAAAAATTATTTAAATATAATAGTTAAGGATAATGGTAGTGGAATTCCAGTAGAATATAGAGAA
AAAATATATCATCAAGGTTTTTCAACAAAGGAAGCACAGCGTGGATATGGAATGTATATAGTAAAAAAAATAATTGATGA
GACTAAAGGAACAATAAGACTTGATGTGGACAACGGTGTTATTTGGAATATAACTATACCGATGACAAGGGGTGATAAAA
TTGATTCAGGTAATGATAGTTGA

Upstream 100 bases:

>100_bases
ATTAATCTTTATATGTGTATCTATTGTCTTTTATGATTTCATGTTTTTTAAATTTGTAATTGTAAAATCTAAATTTATAT
ATTAAGGCTAAGGTGAGAAT

Downstream 100 bases:

>100_bases
AGATGATCCTATGGTTAGAGAAATAAATTCTAAATTTTTAAAAAGAGTAGAAGGATTTGTTTTGTATAAAGCAGTTTCCA
ATCTTTATGACGCTAAAAAA

Product: putative sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTQIVDALEEKDPNGKIGPYINMQL
KNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVVKEGETYISEATGTLGKSMRAFAPIYDRENKKEIGFVSVGT
LNENIETAKHIAILYIILIGFGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRIPIMKREKIVGAIASFRDKTE
VTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEEYDEALQFISDMAKSRNSISNILSHNIKNPSISALLLSKYN
KAEECRVSLKIDENCKLTKLPQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
KIYHQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS

Sequences:

>Translated_540_residues
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTQIVDALEEKDPNGKIGPYINMQL
KNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVVKEGETYISEATGTLGKSMRAFAPIYDRENKKEIGFVSVGT
LNENIETAKHIAILYIILIGFGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRIPIMKREKIVGAIASFRDKTE
VTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEEYDEALQFISDMAKSRNSISNILSHNIKNPSISALLLSKYN
KAEECRVSLKIDENCKLTKLPQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
KIYHQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS
>Mature_540_residues
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTQIVDALEEKDPNGKIGPYINMQL
KNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVVKEGETYISEATGTLGKSMRAFAPIYDRENKKEIGFVSVGT
LNENIETAKHIAILYIILIGFGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRIPIMKREKIVGAIASFRDKTE
VTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEEYDEALQFISDMAKSRNSISNILSHNIKNPSISALLLSKYN
KAEECRVSLKIDENCKLTKLPQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
KIYHQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=540, Percent_Identity=31.6666666666667, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1786837, Length=536, Percent_Identity=26.865671641791, Blast_Score=227, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60657; Mature: 60657

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTQIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DALEEKDPNGKIGPYINMQLKNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVV
HHHHCCCCCCCCCCEEEHHHHCCCEEEEEEEECCCCEEECCCCHHHHHHHCCCCCCEEEE
KEGETYISEATGTLGKSMRAFAPIYDRENKKEIGFVSVGTLNENIETAKHIAILYIILIG
ECCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
FGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
HHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEE
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRI
ECCCEEEEEHHHHCCHHHHHHCHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCEEEECCC
PIMKREKIVGAIASFRDKTEVTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YDEALQFISDMAKSRNSISNILSHNIKNPSISALLLSKYNKAEECRVSLKIDENCKLTKL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEECC
PQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
CHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHH
KIYHQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS
HHHHCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRKAMKLQTKITLLIITVVFLSIAIIISFVVSWMTGNIENKAKTNIMNVAEMVAHSTQIV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
DALEEKDPNGKIGPYINMQLKNLQQIQYIIVADNDGIRYSHPNPKMIKKKFEGGDEYKVV
HHHHCCCCCCCCCCEEEHHHHCCCEEEEEEEECCCCEEECCCCHHHHHHHCCCCCCEEEE
KEGETYISEATGTLGKSMRAFAPIYDRENKKEIGFVSVGTLNENIETAKHIAILYIILIG
ECCCHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
FGGLMAGTIGAILLSNNIKNILLGLEPEEITNLYNEKMGILDTIHEGLVAVDYMGRITLI
HHHHHHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEE
NNSALKILNLQHKVDKDEVIGKNIENIIENTGMINILQTGESEFEREQKINNTTIMTNRI
ECCCEEEEEHHHHCCHHHHHHCHHHHHHHCCCEEEEEECCCHHHHHHHHCCCCEEEECCC
PIMKREKIVGAIASFRDKTEVTRMAEELTGAKKMAWSLRAQNHEFMNKLHTISGLIQLEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YDEALQFISDMAKSRNSISNILSHNIKNPSISALLLSKYNKAEECRVSLKIDENCKLTKL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCEECC
PQHMTSEEIVSIIGNLIENSLDEVKNDGTGLIYIKIVENKNYLNIIVKDNGSGIPVEYRE
CHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHH
KIYHQGFSTKEAQRGYGMYIVKKIIDETKGTIRLDVDNGVIWNITIPMTRGDKIDSGNDS
HHHHCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]